&lt;i&gt;In vitro &lt;/i&gt;DNA Synthesis in Lymphocytes from DNCB Contact-Sensitive Guinea Pigs

Polák, L.

doi:10.1159/000163000

Cited by 1 publication

(1 citation statement)

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“…In patients with chronic berylliosis, we have found positive lymphocyte responses to BeS04 and BeF [19], but not to BeO (unpublished observa tions), indicating perhaps that the molecular configu ration of the initial sensitizing hapten as well as the nature of the carrier [20] may be critical for secondary recognition in vitro. Some investigators, however, have considered the absence of in vitro lymphocyte responsiveness to beryllium salts to be related to chemical toxicity [21].…”

Section: Discussionmentioning

confidence: 77%

Experimental Beryllium-Induced Lung Disease

Barna

Deodhar

Chiang

et al. 1984

Int Arch Allergy Immunol

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Granulomatous lung disease was produced in strain 2 but not strain 13 guinea pigs by endotracheal injection of beryllium oxide (BeO), although isolated multinucleated giant cells containing crystalline material were found in lungs of both strains. Disease was also produced in (2 × 13) F₁ hybrids, but of a milder form than in strain 2. In BeO-treated strain 2 and F₁ animals, challenge with beryllium salts produced delayed hypersensitivity skin reactivity, and in vitro, enhanced tritiated thymidine uptake and lymphokine production by lymph node cells. No evidence was found for involvement of humoral immunity in the disease. Responses were not seen in BeO-treated strain 13 guinea pigs, and immunization of animals with BeO in complete Freund’s adjuvant produced similar immunologic findings. These data emphasize the involvement of genetically determined cellular immune mechanisms in beryllium lung disease.

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Section: Discussionmentioning

confidence: 77%