<I>In vitro</I> effect of hesperidin on root dentin collagen and de/re-mineralization

Abstract

The aims of this study were to investigate the effects of hesperidin, a citrus flavonoid, on human root dentin demineralization and collagen preservation, and compare it with chlorhexidine and grape seed extract. Specimens were assigned to different treatment groups: hesperidin, chlorhexidine and grape seed extract. Specimens were subjected to pH cycling by demineralization for 14 h, incubation in testing solutions for 2 h and remineralization in presence of bacterial-derived collagenase for 8 h, for 8 days. Calcium release was measured by means of an atomic absorption spectrophotometer, and degraded collagen matrix was investigated by hydroxyproline assay. Specimens were assessed longitudinally with transverse micro-radiography to investigate lesion depth and mineral loss. In hesperidin and grape seed extract groups, demineralization was reduced when the collagen matrix was preserved. The hesperidin group showed the lowest value in lesion depth and mineral loss, indicating that hesperidin inhibited demineralization and probably enhanced remineralization even under fluoride-free conditions.

State of the Art Enamel Remineralization Systems: The Next Frontier in Caries Management

“…G. chinensis remineralization is believed to be mediated through different polyphenol compounds that act as Ca 2+ ion carriers into the lesion body [Cheng et al, 2015]. Hesperidin, a citrus flavonoid, and gum arabic, an Acacia exudate, are other natural products that have been found to suppress aciddependent demineralization and boost remineralization even under fluoride-free conditions [Islam et al, 2012;Onishi et al, 2008].…”

Natural Products and Caries Prevention

“…It has also been reported that hesperidin reduced the susceptibility of dentine lesions to acid-dependent demineralization with the potential to promote the remineralization process [Hiraishi et al, 2011]. In another paper, hes- peridin was found to reduce lesion depth and mineral loss, indicating that hesperidin inhibited demineralization and probably enhanced remineralization even under fluoride-free conditions [Islam et al, 2012]. The mechanism of hesperidin may be related to its interaction with collagen and/or noncollagenous proteins, resulting in stabilizing the collagen matrix and induction of remineralization [Liu et al, 2004].…”

Pull-out bond strength of a self-adhesive resin cement to NaOCl-treated root dentin: effect of antioxidizing agents

“…A number of natural flavonoids, such as GSE and hesperidin (HPN), have been used on root caries to study their cross-linking effects on demineralized lesions in order to render collagen matrix more stable 18. Flavonoids are polyphenolic compounds in plant-derived food products.…”

“…In addition, HPN affects bone formation 18,20. Islam et al applied HPN to tooth structures in an in vitro caries model and concluded that its cross-linking properties might inhibit degradation of collagen and demineralization of bovine root dentin 18…”

“…A recent study on HPN showed that it protects bovine dentin collagen against proteolytic activities 18. Moreover, HPN prevents demineralization of dentin by acid and has the capacity to help remineralize dentin 21,22.…”