&lt;i&gt;In vitro&lt;/i&gt; evaluation of crude extracts of &lt;i&gt; Catharanthus roseus&lt;/i&gt; for potential antibacterial activity

Goyal, Pankaj; Khanna, Arjun; Chauhan, Abhishek; Chauhan, Garima; Kaushik, Purshotam

doi:10.4103/0973-8258.42739

Cited by 46 publications

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“…17, 18 The effectiveness of the preparation might be influenced by the extraction method. Antimicrobial activity of medicinal plants has been demonstrated to be higher in organic extracts than aqueous extracts 20,21 .…”

Section: Discussionmentioning

confidence: 99%

In vitro study to determine antimicrobial activity of selected Ayurvedic preparations against bacteria and fungi causing superficial skin infections

Gomes

Hewageegana

Kottahachchi

et al. 2013

Sri Lankan J Infec Dis

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The increasing antimicrobial resistance exhibited by microorganisms causing superficial skin infections has led to extensive research on the therapeutic potential of Ayurvedic preparations. Medicinal plants contain many types of naturally occurring and side effectsfree anti microbial compounds that can be effectively used against microbial infections. We tested the antimicrobial activity of twenty-eight Ayurvedic preparations used to treat superficial infections in a local Ayurvedic healthcare institution. They were tested against Trichophyton rubrum, Microsporum gypseum, Candida albicans, Malassezia furfur, Staphylococcus aureus and Streptococcus pyogenes. Twelve preparations showed significant antimicrobial activity and gave inhibition zones >10 mm. Two Ayurvedic preparations (Mixture containing Terminella chebula, Terminella bellerica and Emblica officinalis and one of Terminella chebula only ) showed antimicrobial activity against all the microorganisms tested. We suggest that these two decoctions could be effectively used against all the above tested strains.

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Section: Discussionmentioning

confidence: 99%

In vitro study to determine antimicrobial activity of selected Ayurvedic preparations against bacteria and fungi causing superficial skin infections

Gomes

Hewageegana

Kottahachchi

et al. 2013

Sri Lankan J Infec Dis

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“…Gram negative bacteria are reported to be resistant against most antibacterial agents as a result of the more complicated nature of their cell wall compared to Gram positive bacteria [9,15,16]. However, Spondias mombin was found to be active against the two groups of Clinical and Environmental Isolates underlining their ethnomedicinal use for treatment of various infectious diseases [17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Antifungal Efficacy of Partially Partitioned Fractions of Spondias mombin (Linn) Extracts (Root, Leaf and Stem Bark) against Clinical and Environmental Isolates

Osuntokun¹,

Idowu²,

Cristina³

2018

Med chem

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The purpose this research work is to determine the antibacterial and antifungal assay of partitioned fractions of S. mombin (Linn) extracts against clinical and environmental isolates. The root, leaf and stem-bark of S. mombin were harvested and air-dried. The dried S. mombin was milled into powdered form using manual grinder. Powdered S. mombin (1 kg) each of the different S. mombin parts was extracted with 3 L of 70% (v/v) ethanol, ethyl acetate and distilled water for 72 h at room temperature. The SMRE and SMREA were used to code for root part; SMLE and SMLEA for the leaf part; and SMSBE and SMSBEA for the stem-bark part, each was fractionated on column chromatography with silica as the stationary phase using n-hexane, ethyl acetate and ethanol as the eluting solvent to obtain n-hexane, ethyl acetate and ethanol fractions. Antimicrobial and antifungal screened was observed using agar well diffusion test. The result obtained showed that in partially purified ethyl acetate leaf extracts, Fraction (F 1 SMLEAH) showed significant inhibitory effect (p ≤ 0.05) on all the test bacteria, except Klebsiella pneumoniae and Salmonella typhi at concentrations of 20.0-2.5 mg/ml, Fraction (F 2 SMLEAEA) was not effective against Salmonella cholleraesuis, B. substilis, Citrobacter koseri and Salmonella typhi. Fraction (F 3 SMLEAE) showed little or no inhibitory effect on most of the bacteria at all the concentration used. It can be deduced that in partially purified ethanolic leaf extracts, Fraction (F 1 SMLEH) showed inhibitory effect on Burkholderia cepacia. All the organisms were not susceptible to all the fractions except F 1 which had diameter of zones of growth inhibition ranging between 4.0-1.0 mm at 5 mg/ml-0.625 mg/ml on Mycobacterium abscessus. Partially purified ethanolic stem bark extracts, antifungal activity of the partially purified ethanolic extracts of S. mombin, Fractions (F 1 SMLEH, F 2 SMLEEA) and F 3 SMLEE), significant antifungal activity (p ≤ 0.05) was observed at 20.0 mg/ml with most test fungi. Trichoderma horizionum was not susceptible to all the three fractions, while Aspergillus niger and Syncephala strumracemosum were susceptible to only fraction (F 2 SMLEEA). Fractions (F 1 SMSBEH, F 2 SMSBEEA), F 3 SMSBEE) on the test as the eluting solvent. Significant inhibitory effect (p ≤ 0.05) was observed in all the fractions at 20 mg/ml against most of the test bacteria. While, zones of growth inhibition of the various fractions varied with the test bacteria with the highest diameter zone of 8.0 mm recorded in fraction F 1 against Salmonella typhi. Fractions (F 2 SMSBEEA and F 3 SMSBEE) possessed significant inhibitory effect (p ≤ 0.05) at 20.0-5.0 mg/ml on the test fungi, except Candida kruise and Rhizopus stonifer. The plant part by solvent interactive effect was significant (p<0.05), suggesting that the MICs and MBCs of test bacteria were observed at 0.3125 and 0.1562 mg/ml and MICs and MFCs test fungal were observed at 0.3125 and 0.1562 mg/ml respectively, The various plants differ significant...

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“…Medicinal plants, which have been used in virtually all cultures for centuries, may be regarded as a reservoir of biologically active compounds, including those possessing antimicrobial activity [1][2][3][4][5][6][7]. According to our results, the crude ethanol extracts obtained from the roots of Rheum palmatum, Rheum undulatum and Rheum rhaponticum growing in Poland inhibited the growth of staphylococcal reference strains at concentrations ranging from 125 to >500 mg/mL; the R. undulatum extract exerted the strongest inhibitory effect against staphylococci with MICs ranging from 125 to 250 μg/mL, suggesting its moderate in vitro bioactivity.…”

Section: Discussionmentioning

confidence: 99%

“…Plants have been shown to be a potential source for multiple antimicrobial agents, as they produce a wide variety of secondary compounds as natural protection against microbial attack [1][2][3][4][5][6]. The use of plants or plant preparations to treat infections has been a common practice in virtually all cultures for a long time [6,7].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity and total content of polyphenols of Rheum L. species growing in Poland

2010

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Abstract:In the crude ethanol extracts obtained from the rhizome and roots of Rheum palmatum L., Rheum undulatum L. ) than against those of Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis).The strongest inhibitory effect against Staphylococcus spp. was exerted by R. undulatum extract with MIC = 125-250 μg/mL. The moderate in vitro antibacterial activity of R. undulatum suggests that this plant, often used in the European cuisine to improve flavour, may be also important and useful as an alternative or auxiliary medicine remedy in the treatment of uncomplicated superficial infections caused especially by clinically important staphylococci, potentially pathogenic S. aureus or opportunistic S. epidermidis.

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<i>In vitro</i> evaluation of crude extracts of <i> Catharanthus roseus</i> for potential antibacterial activity

Cited by 46 publications

References 0 publications

In vitro study to determine antimicrobial activity of selected Ayurvedic preparations against bacteria and fungi causing superficial skin infections

In vitro study to determine antimicrobial activity of selected Ayurvedic preparations against bacteria and fungi causing superficial skin infections

Antibacterial and Antifungal Efficacy of Partially Partitioned Fractions of Spondias mombin (Linn) Extracts (Root, Leaf and Stem Bark) against Clinical and Environmental Isolates

Antimicrobial activity and total content of polyphenols of Rheum L. species growing in Poland

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