&lt;i&gt;In Vitro&lt;/i&gt; Mass Propagation of &lt;i&gt;Mimosa pudica&lt;/i&gt; L., Using Shoot Tip and Nodal Explants

Hassan, Akm Sayeed; Sultana, Rebeka; Jahan, Miskat Ara Akhter; Khatun, Rahima

doi:10.3329/bjsir.v45i2.5704

Cited by 6 publications

(8 citation statements)

References 19 publications

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“…The results found in our study show that the adventitious rooting developed at in vitro phase under stimulus of auxins was irrelevant, since both rooted and non-rooted micro-cuttings of M. pudica presented high survival rate to acclimatization. Moreover, the success in the acclimatization phase found in the present study was better than those found in other studies with M. pudica (Hassan et al, 2010;Ramesh et al, 2013). The plantlets mortality observed during the in vitro rooting stage was higher than in acclimatization phase.…”

Section: The Procedures Of Acclimatizationcontrasting

confidence: 89%

“…Differences were observed for the Acacia mangium Willd for which the supplementation of Bianchetti et al 425 0.5 µM NAA to the culture medium for multiple shoots induction (4 µM BAP) was harmful, leading to the reduction of shoots proliferation (Shahinozzaman et al, 2012). For M. pudica cultures, Hassan et al (2010) found high multiplication rates when 6.6 µM BAP and 2.6 µM NAA were combined, although the response percentages have been smaller. Also, studying M. pudica micropropagation, Ramesh et al (2013) achieved an increase in number of shoots/explant using simultaneously IAA and NAA in culture media supplemented with BAP.…”

Section: Cultured In Vitromentioning

confidence: 83%

“…M. pudica is used in folk medicine, mainly in Asian countries, whereas its extracts are attributed diuretic, astringent and antispasmodic activities. Its roots and leaves are used in hemorrhoids and fistula treatments and against convulsions (Hassan et al, 2010). In vitro and in vivo analysis of flavonoids and phenolic compounds of leaves, stems, and seeds of the M. pudica have found high antioxidant, antimicrobial, anticancer and antidiabetic activities, justifying its use in different diseases treatments (Doss et al, 2011;Kaur et al, 2011;Zhang et al, 2011;Joseph et al, 2013;Gunawardhana et al, 2015;Tunna et al, 2015;Jose et al, 2016;Muhammad et al, 2016;Patro et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Considering its pharmacological and medicinal properties and its ecological relevance, there are, relatively, few studies involving large-scale micropropagation of M. pudica (Ramakrishna et al, 2009;Hassan et al, 2010;Ramesh et al, 2013). Micropropagation allows the production of genetically uniform plants in reduced space and in controlled conditions of temperature, photoperiod and luminosity (Parveen and Shahzad, 2011;Varshney and Anis, 2012).…”

Section: Introductionmentioning

confidence: 99%

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An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

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This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

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Section: The Procedures Of Acclimatizationcontrasting

confidence: 89%

Section: Cultured In Vitromentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The highest percentage (96%) of root formation, maximum number of roots (8.70±0.42) and highest length (4.64±0.23) of roots were recorded in this medium within three weeks of inoculation (Plate 5). Several earlier researchers (Khalekuzzaman et al, 2008, Biwas et al, 2009, Hassan et al, 2010, Das et al, 2008, Karthikeyan et al, 2009 reported root induction in different medicinal plants by using IBA in MS and modified MS. In the present study it was observed that IAA and NAA were less effective for root induction in E. alba.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

Yesmin¹,

Hashem²,

Islam³

2016

Jahangirnagar Univ. J. Biol. Sci.

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Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.

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An efficient high-frequency direct organogenesis protocol of Mimosa pudica, suitable for mass multiplication and transformation experiments

Kavane,

Raval,

Joshi

et al. 2023

Braz. J. Bot

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Mimosa pudica is a creeping perennial, owering medicinal plant belonging to the family Mimosaceae. The plants have been well documented for their various valuable properties such as antiseptic, antimicrobial, antiviral, antimalarial, hyperglycemic, diuretic, anti-in ammatory, antihepatotoxic, anticancer and immune boosters. Due to the occurrence of various phytochemicals, the plant has been extensively used in AYUSH for the treatment of various ailments. Due to the immense medicinal properties of the plant and high exploitation, may lead to a high risk of drastic decreases in its population from natural habitats. Hence conservation of the plant is the utmost requirement by using plant tissue cultures protocols. In this investigation, we have developed a direct organogenesis protocol for the plant using apical meristem as the source of explant. From the tested 12 hormonal combinations; MPO4 (Kn 2.0 + BAP 1.0 + 2,4 D-0.2 mg/lit) was able to generate a signi cantly higher number of multiple shoots (44.33/explant) and the lowest multiple shooting (0.67/explant) was noticed in MPO12 media. Out of 15 media combinations, the MPR10 (NAA 1.0 mg/lit) combination was the best for in-vitro rooting and was able to induce a signi cantly higher percentage (72.67% explants show rooting induction) of adventitious rootings, followed by MPR3 (IBA 1.5 mg/lit) media (62.67% 72.67% explants shows rooting). In this investigation, the rooted plants were successfully hardened to the eld. The developed direct organogenesis protocol using apical meristem as an explant is the rst time report for the mentioned plant and may be used for mass multiplication as well as plantlet production after genetic transformations.

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<i>In Vitro</i> Mass Propagation of <i>Mimosa pudica</i> L., Using Shoot Tip and Nodal Explants

Cited by 6 publications

References 19 publications

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

An efficient high-frequency direct organogenesis protocol of Mimosa pudica, suitable for mass multiplication and transformation experiments

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