<i>IN VITRO</i> TESTING OF MODIFIED COLLAGEN/ HYALURONAN/BETA-GLUCAN SCAFFOLD

Abstract

Cells and scaffolds belong to main components of tissue engineering. In this study we have performed in vitro cytotoxicity and biocompatibility testing of modified collagen/hyaluronan/beta-glucan scaffold. We used direct contact assay as well as MTT test. Human adipose tissue-derived stem cells were used as biological model. According to obtained results, we can summarize, that modified collagen/hyaluronan/beta-glucan scaffold is nontoxic and biocompatible and after further testing it should be used as carrier of various types of cells.

Tissue Engineering of Articular Cartilage: A Mini-Review

“…They are responsible for production of ECM components. Moreover, they are involved in the maintaining and remodelling of the articular cartilage (Cucchiarini et al, 2012;Danisovic et al, 2013).…”

“…Somatic Stem Cells (SSCs) because of their biological characteristics represent another promising tool for cartilage tissue engineering. SSCs are undifferentiated cells with unique potential of selfrenewing and plasticity (Danisovic et al, 2011(Danisovic et al, ). 2013).…”

“…The most commonly used natural material is collagen which belongs to basic constituents of cartilage in vivo. The mechanical properties of collagen-based scaffolds may be easily controlled by chemical modifications (Danisovic et al, 2013). It was shown that chondrocytes cultured within collagen scaffolds maintain their original phenotype and production of Glycosaminoglycans (GAGs) under in vitro conditions.…”

Enhanced differentiation of osteoblastic cells on novel chitosan/ β -1,3-glucan/bioceramic scaffolds for bone tissue regeneration

“…However, according to the available literature, there are few papers describing biomaterials comprising other glucans, e.g. bio-artificial skin composed of gelatin and (1,3)-(1,6)-β-glucan [17], collagen/hyaluronan/βglucan scaffold for regenerative medicine applications [18] or PGLA scaffold coated with (1,3)-(1,6)-β-glucan for skin wound healing that was unexpectedly proven to retard fibroblast proliferation [19].…”