<i>L</i>-Histidine Inhibits the Growth of Ehrlich Ascites Tumour

Abstract

The in vitro studies on the growth of cultured Ehrlich ascites tumour cells showed similar results as the in vivo studies reported previously. The growth of tumour cells was inhibited when cultured in 0.02 or 0.03 M L-histidine. At these concentrations, L-glycine shows no significant effect.

Histamine and serotonin inhibit induction of ornithine decarboxylase by ornithine in perifused Ehrlich ascites tumour cells

“…The inhibitory effect of the amino acid histidine is probably caused via histamine, because cancer cells show an inducible histidine decarboxylase activity [28]. This effect of histidine on the ornithine decarboxylase induction might explain the inhibition of cell growth caused in cultured Ehrlich ascites cells by histidine [29].…”

Effect of <i>L-</i>and D-Lysine on the Growth of Ehrlich Ascites Tumour

“…Recently, L-histidine has been shown to suppress Ehrlich ascites tumour cell growth both in vivo [Choy et al, 1983] and in vitro [Choy et al, 1986], L-lysine shows the same effect as L-histidine on tu mour growth in vivo and in vitro, while Dlysine has no effect ( fig. 1), indicating that the /.-configuration is essential.…”

“…The anti-proliferative effect of the amino acid was determined by the method of Beksei [1969] with modification [Choy et al, 1986]. The viabilities of the treated and untreated tumour cells were measured by the dye exclusion technique using 0.1% methylene blue as viable stain.…”

“…For example, arginine was found to have growth-inhibiting effects on UCLA fibrosarcoma [Levy et al, 1954], mam mary tumour in rats [Takeda et al, 1975] and also on sarcoma-180 [Kojina et al, 1973], myeloma tumour [Pryme, 1976] and Ehrlich ascites tumour in mice [Pryme, 1978;Milner and Stepanovich, 1978]. Re cently, L-histidine was found to inhibit the growth of Ehrlich ascites tumour in mice either in vivo [Choy et al, 1983] or in vitro [Choy et al, 1986]. The mechanism re 1 Supported in part by World Health Foundation (H.K.).…”