&lt;i&gt;Morchella nipponensis &lt;/i&gt;sp. nov. (&lt;i&gt;Ascomycota&lt;/i&gt;, &lt;i&gt;Pezizales&lt;/i&gt;): a paleoendemic species of section &lt;i&gt;Morchella&lt;/i&gt; discovered in Japan

Clowez, Philippe; Izumi, Teruo; Lamiable, Paul-Bill; Shibakusa, Koichi; Minculeasa, Camelia; Alvarado, Pablo

doi:10.47371/mycosci.2022.08.005

Cited by 4 publications

(2 citation statements)

References 48 publications

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“…Species of Morchella have previously been shown to harbor eight ascospores per ascus (Clowez et al, 2014(Clowez et al, , 2015Taşkin et al, 2015;Loizides et al, 2016;Du et al, 2019), but we found that the number of ascospores produced in asci of M. galilaea varied from one to 16 (Figure 5). The number of ascospores produced in asci studied are shown in Table 1.…”

Section: Variation In Ascospore Number In Morchella Galilaeamentioning

confidence: 52%

“…In Morchella species, mature ascospores are typically elliptical (Clowez et al, 2014(Clowez et al, , 2015Taşkin et al, 2015;Loizides et al, 2016;Du et al, 2019), however, various ascospore shapes were observed among the specimens of M. galilaea (Figure 3). Elliptical ascospores dominated and accounted for 96.1% (total 845 spores investigated from the five samples, detailed information shown in Table 1), but irregular shapes, including spherical, heart-shaped, crescent, and other forms were also observed in all investigated fruiting bodies (Table 1, Figure 3).…”

Section: Ascospore Shape and Size Are Variable In Morchella Galilaeamentioning

confidence: 99%

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Cytological studies reveal high variation in ascospore number and shape and conidia produced directly from ascospores in Morchella galilaea

Du,

Wang,

Ryberg

et al. 2023

Front. Microbiol.

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Spores are important as dispersal and survival propagules in fungi. In this study we investigated the variation in number, shape, size and germination mode of ascospores in Morchella galilaea, the only species of the genus Morchella known to fruit in the autumn. Based on the observation of five samples, we first discovered significant variation in the shape and size of ascospores in Morchella. One to sixteen ascospores were found in the asci. Ascospore size correlated negatively with ascospore number, but positively with ascus size, and ascus size was positively correlated with ascospore number. We noted that ascospores, both from fresh collections and dried specimens, germinated terminally or laterally either by extended germ tubes, or via the production of conidia that were formed directly from ascospores at one, two or multiple sites. The direct formation of conidia from ascospores takes place within asci or after ascospores are discharged. Using laser confocal microscopy, we recorded the number of nuclei in ascospores and in conidia produced from ascospores. In most ascospores of M. galilaea, several nuclei were observed, as is typical of species of Morchella. However, nuclear number varied from zero to around 20 in this species, and larger ascospores harbored more nuclei. One to six nuclei were present in the conidia. Nuclear migration from ascospores to conidia was observed. Conidia forming directly from ascospores has been observed in few species of Pezizomycetes; this is the first report of the phenomenon in Morchella species. Morphological and molecular data show that conidial formation from ascospores is not found in all the specimens of this species and, hence, is not an informative taxonomic character in M. galilaea. Our data suggest that conidia produced from ascospores and successive mitosis within the ascus may contribute to asci with more than eight spores. The absence of mitosis and/or nuclear degeneration, as well as cytokinesis defect, likely results in asci with fewer than eight ascospores. This study provides new insights into the poorly understood life cycle of Morchella species and more broadly improves knowledge of conidia formation and reproductive strategies in Pezizomycetes.

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Section: Variation In Ascospore Number In Morchella Galilaeamentioning

confidence: 52%

Section: Ascospore Shape and Size Are Variable In Morchella Galilaeamentioning

confidence: 99%

Cytological studies reveal high variation in ascospore number and shape and conidia produced directly from ascospores in Morchella galilaea

Du,

Wang,

Ryberg

et al. 2023

Front. Microbiol.

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First report of Morchella galilaea, an autumn species from Pakistan, based on multilocus DNA sequences, light microscopy, and scanning electron microscopy analyses

Nisa,

Badshah,

Shah

et al. 2024

Microscopy Res & Technique

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Morchella is highly prized for its distinct and delicious taste, making it one of the most sought‐after and valuable macrofungi. Despite Pakistan exporting morels to various European countries, Morchella's diversity in this South Asian country has not been cataloged using multilocus DNA sequence data. Realizing the need for their scientific identity, various collections were commissioned, including those from the low and high altitudinal zones of the Charsadda, Swat, and Poonch districts of Pakistan during autumn of 2015 through 2019. Specimens were identified by sequencing the internal transcribed spacer (ITS) region and portions of the RNA polymerase II largest subunit (RPB1), second largest subunit (RPB2), and translation elongation factor 1‐α (TEF1) loci. Multigene molecular phylogenetic analysis revealed the autumnal collections were Morchella galilaea Masaphy & Clowez. The species was previously documented in the United States, China, Israel, and Turkey. A detailed taxonomic description, light and scanning electron microscopy, and multigene molecular phylogenetic analyses of the species are reported here for the first time from Pakistan. The study underscores the necessity for further surveys of unexplored areas of Pakistan and systematics of Morchella to augment species diversity and their geographic distribution.Research Highlights Specimens of Morchella were collected from low and high altitudinal zones in Pakistan during the autumn. Specimens were assessed using multigene (ITS, RPB1, RPB2, and TEF1) molecular phylogenetic analyses for the first time in Pakistan. The specimens were identified as Morchella galilaea based on multigene molecular phylogenetic analysis. Updated taxonomic descriptions of macro‐ and microanatomical characters were provided for clarity and precision. Hitherto‐unreported shapes of paraphyses and acroparaphyses are observed and thoroughly reported. Scanning electron microscopy (SEM) of an ascospore is provided.

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A new species of true morel from Switzerland: Morchella helvetica , sp. nov

Cravero,

Bonito,

Chain

et al. 2024

Mycologia

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<i>Morchella nipponensis </i>sp. nov. (<i>Ascomycota</i>, <i>Pezizales</i>): a paleoendemic species of section <i>Morchella</i> discovered in Japan

Abstract: The systematics of genus Morchella Dill. ex Pers. have been much clari ed with the aid of molecular biology (Masaphy, Zabari,

Cited by 4 publications

References 48 publications

Cytological studies reveal high variation in ascospore number and shape and conidia produced directly from ascospores in Morchella galilaea

Cytological studies reveal high variation in ascospore number and shape and conidia produced directly from ascospores in Morchella galilaea

First report of Morchella galilaea, an autumn species from Pakistan, based on multilocus DNA sequences, light microscopy, and scanning electron microscopy analyses

A new species of true morel from Switzerland: Morchella helvetica , sp. nov

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