Tayassuidae (peccaries) is a family of artiodactyls exclusively of America, that was widely distributed in North America during the Pleistocene. Nevertheless, records of this family are scarce in Mexico. The only valid species reported from the Late Pleistocene in Mexico were Platygonus compressus and Dicotyles tajacu, based on a few specimens. In this study, we report a new peccary specimen from the Gliptodonte locality (Late Pleistocene, Rancholabrean NALMA), Villaflores municipality, in the southern State of Chiapas. The new specimen consists of a distal part of the left humerus, which shows morphological differences from other North American Pleistocene and recent peccaries (Mylohyus, Platygonus, and Tayassu), allowing positive identification of the collared peccary D. tajacu. Thus, a new record of the species for the State of Chiapas and the first record of the Gliptodonte locality in the municipality of Villaflores is added, expanding the distribution range of the species during the Late Pleistocene in Mexico.