<i>Providencia</i> in retail meats from Guangzhou, China and Osaka, Japan: prevalence, antimicrobial resistance and characterization of classes 1, 2 and 3 integrons

Abstract

Bacteria of the genus Providencia are opportunistic pathogens of clinical significance due to their association with diarrhea and urinary tract infections. The present study was conducted to examine the prevalence and antimicrobial resistance of Providencia spp. in retail meats sold in Guangzhou, China and Osaka, Japan. Out of 158 meat samples including beef, pork and chicken, 67 Providencia (42%) belonging to four species viz., P. alcalifaciens, P. rustigianii, P. stuartii and P. rettgeri were isolated, and most of them were resistant to tetracycline (91%) followed by ampicillin (69%) and streptomycin (49%). Of 67 isolates, 29 (43%) were MDR, which is defined to be resistant to more than three classes of antimicrobials. No statistically significant differences were observed between Chinese and Japanese retail meat samples regarding contamination rate of Providencia spp. as well as frequency of the antimicrobial resistance of the isolates including MDR. Class 1 and/or class 2 integrons were detected in six of the eight isolates that were resistant to more than 4 antimicrobials, however none of the isolates harbored class 3 integron. A P. rustigianii harboring the blaOXA-10 gene was isolated, which is the first report of Providencia with blaOXA-10 gene of food origin. These data suggest that retail meats in China and Japan are substantially contaminated with Providencia spp., which displayed a high frequency of antimicrobial resistance, and establishing the surveillance of Providencia spp., especially antimicrobial resistant one, in retail meats is imperative.

Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq

“…Providencia spp. including P. rettgeri and P. stuartii were isolated from food [ 9 , 15 – 17 , 53 , 54 ]. In Iraq, out of bacterial content of fish gut, P. rettgeri reported 1/50 [ 55 ].…”

“…MDR (43%) showed in Providencia spp. including P. stuartii and P. rettgeri isolated from retail meats, and most of the isolates were resistant (91%) against tetracycline, ampicillin (69%), and streptomycin (49%) [ 54 ].…”

Antibiotic-Resistant Bacteria across a Wastewater Treatment Plant

“…E148 also showed resistance to tetracycline. The resistance of these strains to tetracycline has been previously reported [49][50][51][52]. The PCR screening revealed the presence of tet(M) in 15 isolates, of which 11 were confirmed by sequencing (Table 3).…”

Bronchopneumonia in sheep associated with Providencia stuartii

“…In addition, Providencia spp. were found in retail meats in China and Japan (Di et al, 2018) and Barbour et al (2012) isolated of liver from broiler chicken in Lebanon, which could cause a serious public health problem Therefore, this report describes the pathological findings, microbiological and molecular analyses of P. stuartii present in the lung of sheep with bronchopneumonia, highlighting that animals may be important sources of transmission to humans, in addition to enabling the spread of Providencia spp. in food.…”