&lt;i&gt;S&lt;/i&gt;-genotype identification based on allele-specific PCR in Japanese pear

Nashima, Kenji; Terakami, Shingo; Nishio, Sogo; Kunihisa, Miyuki; Nishitani, Chikako; Saito, Toshihiro; Yamamoto, Takatsugu

doi:10.1270/jsbbs.65.208

Cited by 21 publications

(11 citation statements)

References 39 publications

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“…1 ). Several methods using DNA markers have been developed that are capable of specifically discriminating S 1 – S 9 , S k , and S 4 sm ( Ishimizu et al 1999 , Kim et al 2007 , Nashima et al 2014 , 2015 , Okada et al 2008 , Takasaki et al 2004 ).…”

Section: Self-compatibilitymentioning

confidence: 99%

Advances in Japanese pear breeding in Japan

Saito

2016

Breed. Sci.

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The Japanese pear (Pyrus pyrifolia Nakai) is one of the most widely grown fruit trees in Japan, and it has been used throughout Japan’s history. The commercial production of pears increased rapidly with the successive discoveries of the chance seedling cultivars ‘Chojuro’ and ‘Nijisseiki’ around 1890, and the development of new cultivars has continued since 1915. The late-maturing, leading cultivars ‘Niitaka’ and ‘Shinko’ were released during the initial breeding stage. Furthermore, systematic breeding by the Horticultural Research Station (currently, NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NIFTS)) began in 1935, which mainly aimed to improve fruit quality by focusing on flesh texture and black spot disease resistance. To date, 22 cultivars have been released, including ‘Kosui’, ‘Hosui’, and ‘Akizuki’, which are current leading cultivars from the breeding program. Four induced mutant cultivars induced by gamma irradiation, which exhibit some resistance to black spot disease, were released from the Institute of Radiation Breeding. Among these cultivars, ‘Gold Nijisseiki’ has become a leading cultivar. Moreover, ‘Nansui’ from the Nagano prefectural institute breeding program was released, and it has also become a leading cultivar. Current breeding objectives at NIFTS mainly combine superior fruit quality with traits related to labor and cost reduction, multiple disease resistance, or self-compatibility. Regarding future breeding, marker-assisted selection for each trait, QTL analyses, genome-wide association studies, and genomic selection analyses are currently in progress.

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Section: Self-compatibilitymentioning

confidence: 99%

Advances in Japanese pear breeding in Japan

Saito

2016

Breed. Sci.

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“…Identification of the S-genotype is important for commercial fruit production and cross breeding. Several identification systems for rapid and reliable S-genotyping have been established, such as PCR-restriction fragment length polymorphism (PCR-RFLP) analysis (Ishimizu et al, 1999) and allele-specific PCR amplification (Nashima et al, 2015). A self-compatible mutant 'Osa-Nijisseiki' derived from a self-incompatible cultivar 'Nijisseiki' possesses the S 4 sm haplotype controlling selfcompatibility.…”

Section: Mas In Japanese Pear Breeding Programsmentioning

confidence: 99%

DNA Markers and Molecular Breeding in Pear and Other Rosaceae Fruit Trees

Yamamoto

2021

Hortic J

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Pear (Pyrus spp.) is one of the most important edible fruits belonging to the family Rosaceae. DNA markers, molecular genetics and genomics, and molecular breeding of pear have greatly progressed over the last few decades. The development of reliable DNA markers, such as simple sequence repeats and single nucleotide polymorphisms, has allowed DNA profiling of pear accessions, assessment of the genetic diversity within pear species, and analyses of phylogenetic relationships among pear species. Reference genetic linkage maps and genome-wide molecular markers have enabled practical marker-assisted selection for resistance to black spot and/or pear scab diseases, self-compatibility, harvest time, and fruit skin color in Japanese pear breeding programs. Molecular breeding has been shown to more than triple the selection efficiency of practical breeding compared with conventional breeding. Furthermore, breeding programs using two novel genomics-based approaches-genome-wide association studies and genomic selection-focusing on fruit quality and texture, and quantitative traits for breeding, are in progress. Co-linearity and functional synteny have been identified between pear and apple (Malus × domestica Borkh), and have been used to efficiently predict the function of a gene of interest and develop selection markers in related species.

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“…Allele-specific primers were applied for identifying the single alleles (S1, S6, S7, S8). Nucleotide sequences of PyrusS1, PyrusS6, PyrusS7, and PyrusS8 primers were obtained using Ishimizu et al (1999) and Nashima et al (2015), respectively (Table 1). Optimization of PCR (BioRad T100 TM ) was conducted for used primers as follows: 5X PCR reaction buffer, 25 mM MgCl2, 100 µM dNTP, 5 pmol primer, 5U Taq polymerase and 50-250 ng of genomic DNA was performed at a total volume of 15 µL.…”

Section: Polymerase Chain Reactions (Pcr)mentioning

confidence: 99%