A new species of the genus Typhlocharis Dieck, 1869 (Coleoptera: Carabidae: Anillini) is described (T. deferreri nov. sp.) from the province of Cadiz in the south of the Iberian Peninsula. Its affinities with other species are discussed, mainly those with males having a median tubercle in sternum II: T. bivari Serrano and Aguiar, 2006, T. passosi Serrano and Aguiar, 2005, T. navarica Zaballos and Wrase, 1998 (outereloi group), T. monastica Zaballos and Wrase, 1998 and T. peregrina Zaballos and Wrase, 1998 (monastica group). The value of sexual dimorphism as a phylogenetic and taxonomic tool is revised; seemingly there is no relationship between this kind of sexual dimorphism and the traits that differentiate the established species groups (umbilicate series, type of gonocoxite, dimorphism in tibiae I and II, etc.). The presence and significance of stridulatory organ (pars stridens) is also commented upon, updating and increasing the scarce data available about this structure, poorly considered in the literature, with notes about distribution and relationships of the species which have it.