&lt;p&gt;A network pharmacology approach to explore active compounds and pharmacological mechanisms of epimedium for treatment of premature ovarian insufficiency&lt;/p&gt;

Zhao, Huishan; Shan, Yinghua; Ma, Zhi; Yu, Mingwei; Gong, Benjiao

doi:10.2147/dddt.s207823

Cited by 43 publications

(31 citation statements)

References 44 publications

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“…Research conducted with modern technology has confirmed that Chinese medicine plays an important role in delaying aging and treating testicular dysfunction and ovarian insufficiency [30][31][32]. Tonifying kidney recipes (such as Cujing Decoction, Shenqi pill, Yijing Recipe, etc.)…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Heshouwuyin inhibiting the Cyt c/Apaf-1/Caspase-9/Caspase-3 pathway in spermatogenic cell apoptosis

Wang

Zhu

Jiang

et al. 2020

BMC Complement Med Ther

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Background: The Chinese herbal compound Heshouwuyin has been shown to downregulate the apoptotic rate of testicular tissue cells in Wistar naturally aging rats, and this effect might be related to the mitochondrial pathway [15]. Apoptotic protease activating factor-1 (Apaf-1) is a major component of the apoptotic complex, which is a key element of the mitochondrial endogenous apoptotic pathway [13]. To further clarify the mechanism of Heshouwuyin in the mitochondrial apoptotic pathway, this study used Apaf-1 as a target to explore the mechanism by which Heshouwuyin inhibits the Apaf-1 pathway of spermatogenic cell apoptosis. Methods: In this study, an aging model of rat spermatogenic cells was established using free radical oxidative damage. Flow cytometry was used to detect the apoptosis rate of germ cells and the inhibitory effect of Heshouwuyin. Apaf-1 was specifically knocked down by siRNA interference technology, and mitochondrial membrane potential was measured. qRT-PCR, Western blotting and immunofluorescence analyses were used to detect the expression of the key genes Cyt c, Caspase-9 and Caspase-3 in the mitochondrial apoptotic pathway of spermatogenic cells.Results: Heshouwuyin reduced the mRNA and protein expression levels of Cyt c, Caspase-9 and Caspase-3 in senescent spermatogenic cells. In these cells, the mRNA and protein expression levels of Cyt c did not change significantly after specific knockdown of Apaf-1, and the mRNA and protein expression levels of Caspase-9 and Caspase-3 decreased significantly. This finding indicated that knockdown of Apaf-1 could decrease the mRNA and protein expression levels of the downstream pro-apoptotic genes Caspase-9 and Caspase-3. Although Cyt c was an upstream gene of Apaf-1, knockdown of Apaf-1 had no significant effect on Cyt c expression. Conclusion:The inhibition of spermatogenic cell apoptosis by Heshouwuyin was closely related to the Cyt c/Apaf-1/Caspase-9/Caspase-3 pathway. The inhibition of apoptosis by Heshouwuyin not only involved the Apaf-1 pathway, but other signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Mechanism of Heshouwuyin inhibiting the Cyt c/Apaf-1/Caspase-9/Caspase-3 pathway in spermatogenic cell apoptosis

Wang

Zhu

Jiang

et al. 2020

BMC Complement Med Ther

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“…To explore the functional annotation and related gene pathways, (Zhao et al, 2019). Apigenin and luteolin inhibited the AR and AKT signalling network together and increased the therapeutic efficiency of androgen suppression in prostate cancer (Tsai et al, 2017).…”

Section: Gene Ontology and Pathway Analysismentioning

confidence: 99%

Analysis of pharmacological mechanisms of Yinyanghuo as treatment of erectile dysfunction with network pharmacology‐based strategy

et al. 2020

Self Cite

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The definition of erectile dysfunction (ED) encompasses the failure to develop or sustain an erection sufficient enough for sexual activity (Zhou, Chen, et al., 2019). It affects about 50% of men between the ages, 40 and 70, and thus regarded as a health problem of significance (Mitidieri et al., 2020). There are various causes related to ED, for example decrease of testosterone formation in the testis, decline of the amount and receptiveness of neurotransmitters and vasoactive amines, less vascular functionality, diseases (such as chronic prostatitis, diabetes, cardiovascular, depression and hypertension) and certain drugs. ED is considered a important health problem, which not

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“…The target genes of luteolin against in ammation were imported into FunRich software to carry out GO analysis. P value 0.05 were considered to be statistically signi cant for GO enrichment 24 .…”

Section: Gene Ontology (Go) Enrichment Analysismentioning

confidence: 99%

Molecular mechanism of luteolin against inflammation based on integration of network pharmacology, transcriptomics and proteomics

Huang¹,

Zhang²,

Huang³

et al. 2020

Preprint

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Background: Luteolin (3', 4', 5,7-tetrahydroxyflavone), a natural flavonoid exists in various medicinal plants, has strong anti-inflammatory effect. However, anti-inflammatory mechanism of luteolin has not been fully explored. Hence, we aimed to systematically investigate druggability and anti-inflammatory mechanism of luteolin based on network pharmacology. Methods: The absorption, distribution, metabolism, excretion, and toxicity of luteolin were evaluated by TCMSP server. Targets associated with luteolin and inflammation were collected from public databases, and the overlapping targets between luteolin and inflammation were analyzed by Draw Venn diagram. Then the protein-protein interaction network of luteolin against inflammation was constructed to get core genes. Further, gene function and pathway enrichment analysis were performed. Finally, in vitro experiment was carried out to estimate the accuracy of predicted target genes. Results: ADME results indicated that luteolin has great potential to be developed into a drug. 226 overlapping targets (targets of luteolin against inflammation) were screened by matching 280 targets of luteolin with 9015 targets of inflammation. 9 core targets of luteolin against inflammation were identified, including MMP9, MAPK1, HSP90AA1, CASP3, ALB, EGFR, SRC, HRAS and ESR1. Gene function were mainly involved in metabolism, energy pathways and signal transduction. Pathway enrichment results suggested that metabolic pathways, pathways in cancer, PI3K-AKT signaling pathway, Ras signaling pathway and so on might be the critical pathways of luteolin against inflammation. RT-qPCR and ELISA results indicated that luteolin decreased the expression of most of core genes at protein and mRNA levels (MMP9, MAPK1, HSP90AA1, EGFR, SRC and HRAS). Conclusions: The anti-inflammatory mechanism of luteolin were systematically investigated based on network pharmacology, RT-qPCR and ELISA. Luteolin is expounded to have great potential to be developed into a drug and target various genes and pathways to perform systematic anti-inflammatory effect.

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<p>A network pharmacology approach to explore active compounds and pharmacological mechanisms of epimedium for treatment of premature ovarian insufficiency</p>

Cited by 43 publications

References 44 publications

Mechanism of Heshouwuyin inhibiting the Cyt c/Apaf-1/Caspase-9/Caspase-3 pathway in spermatogenic cell apoptosis

Mechanism of Heshouwuyin inhibiting the Cyt c/Apaf-1/Caspase-9/Caspase-3 pathway in spermatogenic cell apoptosis

Analysis of pharmacological mechanisms of Yinyanghuo as treatment of erectile dysfunction with network pharmacology‐based strategy

Molecular mechanism of luteolin against inflammation based on integration of network pharmacology, transcriptomics and proteomics

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