<p>A novel CXCL8 analog is effective in inhibiting the growth via cell cycle arrest and attenuating invasion of Lewis lung carcinoma</p>

Abstract

PurposeLung cancer and other solid tumors contain not only tumor cells but various types of stromal cells, such as fibroblasts and endothelial cells. In addition, tumors are infiltrated by inflammatory cells (neutrophils, macrophages, and lymphocytes). Tumor cells, stromal cells, and the tumor-associated leukocytes are responsible for the production of chemokines inside the tumor and the maintenance of systemic circulating chemokine levels. CXCL8 and its receptors, CXCR1 and CXCR2, were found to play important roles in tumor proliferation, migration, survival, and growth. We have developed a novel ELR-CXC chemokine antagonist CXCL8-IP10 based on the structure of CXCL8 and IP10.Patients and methodsWe assessed the anticancer efficacies of the blockade of CXCL8-CXCR1/2 axis in the Lewis lung carcinoma (LL/2) model using CXCL8-IP10.ResultsWe found that CXCL8-IP10 markedly reduced LL/2 cell anchorage-independent growth and invasion. Moreover, we demonstrated that CXCL8-IP10 could significantly suppress tumor growth and improve survival rate as well as lifespan of C57BL/6 mice inoculated with LL/2 cells.ConclusionOur results suggest that ELR-CXC chemokine antagonism would potentially be a useful therapeutic approach in patients with lung cancer.

Comparison of Cost and Potency of Human Mesenchymal Stromal Cell Conditioned Medium Derived from 2- and 3-Dimensional Cultures

“…IL-8 is implicated in various inflammatory conditions, with increased expression of IL-8 and/or its receptors observed in many chronic inflammatory diseases, often correlating with diseases’ activity [ 62 ]. IL-8 has been suggested as a target for anti-cancer drugs [ 63 , 64 ] and has been associated with adverse outcomes in COVID-19 patients, along with TNF-α, IL-6, IL-1β, and IL-33 [ 65 ]. In the context of cystic fibrosis clinical trials, the IL-8 delta CT post-LPS effect of MSC-CM was assessed as a potency predictor for selecting MSC lots [ 66 ].…”

Implications of ZNF334 gene in lymph node metastasis of lung SCC: potential bypassing of cellular senescence

“…All animal studies were conducted in accordance with local guidelines for the care of laboratory animals at the Isfahan University of Medical Sciences. As many researchers have used the Lewis lung carcinoma cell line (LL/2) with high tumorigenic ability in C57BL and a higher rate of metastases than the original tumor line (LLC1) as a model for NSCLC [ 16 – 18 ], we also obtained this cell line from the national cell bank of the Pasteur Institute (Iran) and plated it as described above (i.e., DMEM/10% FBS at 37 °C in a 5% CO2/95% air atmosphere). LL/2 cells were harvested and resuspended in sterile PBS at a density of 1 × 10 6 cells/100 µL as single‐cell suspensions.…”