&lt;p&gt;A Novel &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;CTX-M-65&lt;/sub&gt;-Harboring IncHI2 Plasmid pE648CTX-M-65 Isolated from a Clinical Extensively-Drug-Resistant &lt;em&gt;Escherichia coli&lt;/em&gt; ST648&lt;/p&gt;

Lü, Yang; Kang, Haiquan; Fan, Jianming

doi:10.2147/idr.s269766

“…We spot a strong association between Enterobacterieae and the enrichment of extended-spectrum β-lactamases and other ARGs in water from hospital surrounds, which differed from waters in wetlands where only 1-2 ARGs were carried by each species. This finding is consistent with previous studies that have reported frequent isolations of ESBL-carrying E. coli from riverine and surface water environments around hospitals (Dirar et al, 2020;Lü et al, 2020). The global dissemination of the bla CTX-M gene has been reported in hospital settings, communities, livestock, and companion animals (Jamborova et al, 2018).…”

Section: Discussionsupporting

confidence: 92%

Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China

Ye

¹

,

Li

²

,

Su

³

et al. 2023

Front. Microbiol.

0

View full text Add to dashboard Cite

The present study investigated the water quality index, microbial composition and antimicrobial resistance genes in urban water habitats. Combined chemicals testing, metagenomic analyses and qualitative PCR (qPCR) were conducted on 20 locations, including rivers from hospital surrounds (n = 7), community surrounds (n = 7), and natural wetlands (n = 6). Results showed that the indexes of total nitrogen, phosphorus, and ammonia nitrogen of hospital waters were 2–3 folds high than that of water from wetlands. Bioinformatics analysis revealed a total of 1,594 bacterial species from 479 genera from the three groups of water samples. The hospital-related samples had the greatest number of unique genera, followed by those from wetlands and communities. The hospital-related samples contained a large number of bacteria associated with the gut microbiome, including Alistipes, Prevotella, Klebsiella, Escherichia, Bacteroides, and Faecalibacterium, which were all significantly enriched compared to samples from the wetlands. Nevertheless, the wetland waters enriched bacteria from Nanopelagicus, Mycolicibacterium and Gemmatimonas, which are typically associated with aquatic environments. The presence of antimicrobial resistance genes (ARGs) that were associated with different species origins in each water sample was observed. The majority of ARGs from hospital-related samples were carried by bacteria from Acinetobacter, Aeromonas and various genera from Enterobacteriaceae, which each was associated with multiple ARGs. In contrast, the ARGs that were exclusively in samples from communities and wetlands were carried by species that encoded only 1 to 2 ARGs each and were not normally associated with human infections. The qPCR showed that water samples of hospital surrounds had higher concentrations of intI1 and antimicrobial resistance genes such as tetA, ermA, ermB, qnrB, sul1, sul2 and other beta-lactam genes. Further genes of functional metabolism reported that the enrichment of genes associated with the degradation/utilization of nitrate and organic phosphodiester were detected in water samples around hospitals and communities compared to those from wetlands. Finally, correlations between the water quality indicators and the number of ARGs were evaluated. The presence of total nitrogen, phosphorus, and ammonia nitrogen were significantly correlated with the presence of ermA and sul1. Furthermore, intI1 exhibited a significant correlation with ermB, sul1, and blaSHV, indicating a prevalence of ARGs in urban water environments might be due to the integron intI1’s diffusion-promoting effect. However, the high abundance of ARGs was limited to the waters around the hospital, and we did not observe the geographical transfer of ARGs along with the river flow. This may be related to water purifying capacity of natural riverine wetlands. Taken together, continued surveillance is required to assess the risk of bacterial horizontal transmission and its potential impact on public health in the current region.

show abstract

“…The similar IncX3 plasmids found in this study further highlight the importance of the epidemic IncX3 plasmid in the spread of the bla NDM-5 gene within the entire ecosystem. IncHI2/ST3 plasmids have been reported to mediate the transfer of various antibiotic resistance genes (ARGs), such as fosA3 ( Wang et al, 2020 ), floR ( Cao et al, 2020 ), bla CTX-M ( Lü et al, 2020 ), and mcr ( Long et al, 2019 ; Zhi et al, 2016 ), as well as various NDM-type carbapenemase genes, such as bla NDM-1 , bla NDM-9 , and bla NDM-4 ( Liu et al, 2017 ; Oueslati et al, 2021 ). However, there are very few reports of bla NDM-5 -carrying IncHI2 ( Ma et al, 2021 ; Zhao et al, 2021b ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of NDM-5-producing Enterobacteriaceae isolates from retail grass carp (Ctenopharyngodon idella) and evidence of blaNDM-5-bearing IncHI2 plasmid transfer between ducks and fish

Lv¹,

Lu²,

Gao³

et al. 2022

Zoological Research

View full text Add to dashboard Cite

We aimed to characterize NDM-5-producing Enterobacteriaceae from aquatic products in Guangzhou, China. A total of 196 intestinal samples of grass carp collected in 2019 were screened for carbapenemase genes. Characterization of bla NDM-5 positive isolates and plasmids was determined by antimicrobial susceptibility testing, conjugation experiments, Illumina HiSeq, and Nanopore sequencing. One Citrobacter freundii and six Escherichia coli strains recovered from seven intestinal samples were verified as bla NDM-5 carriers (3.57%, 7/196). The bla NDM-5 genes were located on the IncX3 ( n =5), IncHI2 ( n =1), or IncHI2-IncF ( n =1) plasmids. All bla NDM-5 -bearing plasmids were transferred by conjugation at frequencies of ~10 −4 –10 −6 . Based on sequence analysis, the IncHI2 plasmid pHNBYF33-1 was similar to other bla NDM-5 -carrying IncHI2 plasmids deposited in GenBank from Guangdong ducks. In all IncHI2 plasmids, bla NDM-5 was embedded in a novel transposon, Tn 7051 (IS 3000 -ΔIS Aba125 -IS 5 -ΔIS Aba125 - bla NDM-5 - ble MBL - trpF - tat -∆ dct -IS 26 -∆ umuD -∆IS Kox3 -IS 3000 ), which was identical to the genetic structure surrounding bla NDM-5 found in some IncX3 plasmids. The IncHI2-IncF hybrid plasmid pHNTH9F11-1 was formed by homologous recombination of the bla NDM-5 -carrying IncHI2 plasmid and a heavy-metal-resistant IncF plasmid through ∆Tn 1721 . To the best of our knowledge, this is the first report on the characterization of bla NDM-5 -bearing plasmids in fish in China. The IncHI2 plasmid pHNBYF33-1 may be transmitted from ducks, considering the common duck-fish freshwater aquaculture system in Guangdong. Tn 7051 is likely responsible for the transfer of bla NDM-5 from IncX3 to IncHI2 plasmids in Enterobacteriaceae , resulting in the expansion of transmission vectors of bla NDM-5 .

show abstract

“…The IncHI2 plasmid is one of the most prevalent mcr-1 -bearing plasmid types, and IncHI2 plasmids are usually MDR plasmids carrying various resistance genes and insertion sequences (ISs) ( 6 , 7 ), especially IS 26 , which is related to cointegration of plasmids ( 8 , 9 ). Importantly, the IncHI2 plasmids were also important vectors for bla NDM-5 , bla CTX-M-65 , and bla OXA-1 , which were great threats to public health ( 10 , 11 ). Plasmid-mediated tigecycline resistance genes tet (X3) and tet (X4) were discovered in China in 2019 ( 12 , 13 ).…”

Section: Introductionmentioning

confidence: 99%

Emerging Opportunity and Destiny of mcr-1 - and tet (X4)-Coharboring Plasmids in Escherichia coli

Lu

¹

,

Xiao

²

,

Lv

³

et al. 2021

View full text Add to dashboard Cite

show abstract

<p>A Novel <em>bla</em><sub>CTX-M-65</sub>-Harboring IncHI2 Plasmid pE648CTX-M-65 Isolated from a Clinical Extensively-Drug-Resistant <em>Escherichia coli</em> ST648</p>

Cited by 9 publications

References 38 publications

Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China

Characterization of microbial community and antibiotic resistome in intra urban water, Wenzhou China

Characterization of NDM-5-producing <i>Enterobacteriaceae</i> isolates from retail grass carp (<i>Ctenopharyngodon idella</i>) and evidence of <i>bla</i><sub>NDM-5</sub>-bearing IncHI2 plasmid transfer between ducks and fish

Emerging Opportunity and Destiny of mcr-1 - and tet (X4)-Coharboring Plasmids in Escherichia coli

Contact Info

Product

Resources

About