<p>A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process</p>

Abstract

Introduction: Chronic skin lesions represent a problem of increasing occurrence, mostly due to the global ageing of the world population. Research in skin care and dermatology is constantly looking for new non-invasive solutions, preferably those based on the use of natural certified products, able to accelerate the spontaneous skin repair mechanisms and without altering the skin normal appearance and functionality. The wound healing process in the skin is finely regulated by several factors and orchestrated mechanisms, which modulate the progression and the fitting of different consequent phases, including haemostasis, inflammation, cell proliferation and tissue remodelling. It was previously shown that a patented Triticum vulgare aqueous extract was able to trigger the skin repair process by stimulating new tissue growth and reducing the expression levels of inflammatory mediators, such as IL-6, TNFα, prostaglandin E2, and nitric oxide. Methods: Scratch assay was performed in Human Dermal Fibroblasts (HDF). The production of fibronectin was measured by gene expression, protein quantification and localization using specific antibodies in HDF. The polymerization of actin was measured using rhodaminphalloidin in HDF. The epidermal lipid content was estimated in HaCaT (human spontaneously immortalized keratinocytes) using Nile Red staining and the increasing GBA gene expression and activity was demonstrated by RT-PCR and enzymatic activity assay. Results: In the present study, it was demonstrated that the T. vulgare extract enhanced cell migration inducing the synthesis of fibronectin, new actin polymerization and stimulating the expression of the Hyaluronan Synthase 2. Furthermore it improved the restoration of the epidermal barrier stimulating lipid synthesis. Conclusion: In conclusion, we demonstrated that the T. vulgare extract possessed promising potential to be developed as a wound healing promoting agent in skin care and dermatology.

Keratin Promotes Differentiation of Keratinocytes Seeded on Collagen/Keratin Hydrogels

“…We believe this is due to the Ker-CT cells possibly undergoing lysosomal biogenesis due to constant proliferation [ 43 , 44 ]. However, a recent study by Tito et al demonstrated increased GBA activity with HaCaT cells when treated with Triticum vulgare, a plant-derived treatment known to accelerate wound healing [ 56 ]. This suggests that an appropriate inducer for increasing GBA activity due to differentiation may be needed to overcome the GBA activity reading from constant proliferation.…”

Comparative study to evaluate the wound healing efficacy of topical formulations containing Triticum aestivum L. (Triticum vulgare) in a native human skin model

“…É um processo dinâmico, desencadeado em resposta à lesão tecidual, que visa reparar a matriz e os danos celulares e restaurar a integridade da barreira cutânea, passando por quatro fases que se sobrepõem: hemostasia, inflamação, proliferação e remodelamento. 9 O processo de cicatrização começa com a fase de hemostasia, que consiste na formação de um coágulo sanguíneo que preenche a lesão, no intuito de estancar o sangramento e preservar as estruturas do tecido. Essa etapa está ligada à formação de uma matriz temporária, à secreção de citocinas e a outros fatores de crescimento que, ao interagirem com os componentes da ma-triz extracelular (MEC), desencadeiam todo o processo de reparo.…”

“…26 Tito e colaboradores também mostraram uma ação do extrato de T. aestivum L. no estímulo da síntese de fibronectina, componente-chave na formação e organização da matriz extra-celular e também da enzima hialuronan sintase 2, precursora do ácido hialurônico. 9 Esses mesmos autores atribuíram ao extrato a propriedade de restauração da barreira cutânea por meio do aumento da síntese de ceramidas. 9 Diversos extratos e frações isoladas de T. aestivum L. foram avaliados e confirmaram a habilidade dessa espécie em diferentes mecanismos envolvidos no processo de regeneração tecidual.…”

In Vitro Antimicrobial Activity Evaluation of a Novel Fitostimoline® Plus Spray Formulation

“…Rigenase ® , a specific patented Triticum vulgare extract, exhibits antioxidant capacity by tissue-repairing activity and moisturizing action [ 18 ]; thus, it is used in the formulation of different medical devices—under the brand name Fitostimoline ® —with properties of accelerating tissue repair, improving the restoration of the epidermal barrier, and stimulating lipid synthesis [ 19 ].…”