&lt;p&gt;An efficient method that combines the ThinPrep cytologic test with E6/E7 mRNA testing for cervical cancer screening&lt;/p&gt;

Dan, Pan; Zhang, Chan-Qiong; Liang, Qiong; Hong, Xiao-Cui

doi:10.2147/cmar.s197749

Cited by 11 publications

(13 citation statements)

References 26 publications

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“…In this study, the positive rate of HPV DNA assay was 85.3%, which was much higher than that of the HPV E6/E7 mRNA test (61.6%).This relatively high percentage of HPV DNA infection in cases with positive cervical cytology is consistent with some previously published studies [22,23,27].There was an association between E6/E7 mRNA expression and histology (OR = 106.12) [28]. Data analysis from our study showed an association between the results of HPV E6/E7 mRNA, histological diagnosis, and the TCT grade.The positive rate of HPV E6/E7 mRNA and histological diagnosis increased with the upgrade of TCT.…”

Section: Discussionsupporting

confidence: 91%

“…There are many studies on the performance of HPV E6/E7 mRNA test in diagnosing cervical diseases. Some reports have suggested that HPV E6 / E7 mRNA test is more sensitive and speci c than the HPV NDA test [22][23][24]. It has also been suggested that the HPV E6/E7 mRNA test is only more speci c than the HPV DNA test,having no difference in sensitivity [25].…”

Section: Discussionmentioning

confidence: 99%

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The diagnostic value of HPV E6/E7 mRNA test in young women with cervical squamous intraepithelial lesion: A Retrospective Analysis

Wang

2021

Preprint

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Background At present, the HPV DNA test is used to triage young female patients with abnormal cytology. Still, it is not suitable to precisely identify the population with persistent HPV infection. The purpose of this study was to evaluate the diagnostic value of HPV E6/E7 mRNA test in young women with abnormal cytology by comparing HPV DNA test.Methods A total of 258 young women aged 20 to 29 years,with squamous cell abnormalities on the cervical cytology, were enrolled in this study between January 2015 and December 2019.All patients were subject to HPV DNA test, HPV E6/E7mRNA test,colposcopy biopsy, and histopathological examination.A comparative analysis of the diagnostic performance of the HPV DNA test and HPV E6/E7mRNA test was conducted according to the histological diagnosis(CIN II and CIN II+were defined as high-grade squamous intraepithelial lesion+(HSIL+)).Results The results showed that HPV E6/E7 mRNA test had a higher specificity of 47.3%(40.0%-55.1%) for HSIL+ compared to HPV DNA test that had specificity of 16.0% (11.0%-22.6%)in young women(P<0.01). The HPV E6/E7 mRNA test presented high rates of specificity,positive predictive value (PPV), and negative predictive value(NPV), which were 92.1%(86.0%-96.0%), 62.1%(42.4%-78.7%), 92.1%(85.9%-95.8%), respectively, compared to that of HPV DNA,which were 15.8%(10.4%-23.2%),14.6%(9.40%-21.9%), and 71.0%(51.8%-85.1%),respectively(P<0.01)in young women with mildly abnormal cytology(ASC-US and LSIL). Yet, with severe abnormal cytology(ASC-H and HSIL), HR-HPV test was similar to HPV E6/E7 mRNA test in sensitivity(χ2=0.98,P=0.322),specificity(χ2=0.938,P=0.333),PPV(χ2=0.074,P=0.786) and NPV(χ2=0.00,P=1.000).Conclusions Compared to the HPV DNA test,the HPV E6 /E7mRNA test has better clinical value in screening cervical cancer and predicting the risk of HSIL+ in young women, especially those with mild abnormal cytology.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

The diagnostic value of HPV E6/E7 mRNA test in young women with cervical squamous intraepithelial lesion: A Retrospective Analysis

Wang

2021

Preprint

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“…Next, the full-text of the 20 articles were reviewed, and different reports of the same clinical study and articles inconsistent with the purpose of this study were excluded. Ultimately, a total of 5 studies were included in the study (11)(12)(13)(14)(15). All the retrieval and screening processes were completed independently by 2 evaluators, and any differences in opinions were resolved through internal discussion (Table 1).…”

Section: Literature Retrieval Results and Included Research Character...mentioning

confidence: 99%

The diagnostic accuracy of TCT + HPV-DNA for cervical cancer: systematic review and meta-analysis

Li¹,

Lai²,

Yuan³

2022

Ann Transl Med

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Background: This study sought to systematically analyze the clinical diagnostic value of tumor markers combined with ThinPrep cytologic test (TCT) and human papillomavirus (HPV) deoxyribonucleic acid (DNA) detection for cervical cancer and pre-cancerous lesions. However, there is a lot of controversy in the field of TCT+HPV-DNA. Many people have mixed opinions on the accuracy of TCT+HPV-DNA, and there is no unified opinion. Therefore, it is necessary to further confirm the significance of this combined detection method in the early diagnosis of cervical cancer by applying meta method.

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“…In this study, we analyzed 1,616 patients who were positive for HPV16/18/52/58 alone with TCT normal, with pathological findings suggestive of normal and inflammatory conditions accounting for 64.89%–78.51%, in line with a TCT specificity of 58%–76% ( 13 ). HPV screening can compensate for the lack of liquid-based cytology and can be combined with liquid-based cytology to improve sensitivity ( 14 ).The pathological findings suggest that HPV58-positive infection is more prevalent in patients with high-grade and above lesions than HPV52 positive and also higher than HPV18 positive. This is consistent with the Kaidar–Person study, which found that HPV58 has a 1.8-fold higher association with invasive cervical cancer than HPV52 ( 15 ).…”

Section: Discussionmentioning

confidence: 99%

Implications of Persistent HPV52 and HPV58 Positivity for the Management of Cervical Lesions

Xu²,

Zhang³

et al. 2022

Front. Oncol.

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ObjectiveThis study aimed to compare the variability of HPV16/18/52/58 subtype infections in patients with different cervical lesions, to explore the guiding significance of persistent positive HPV subtypes 52 and 58 in the stratified management of cervical lesions, and to determine the appropriate management model.MethodThis study was conducted through a retrospective analysis of 244,218 patients who underwent HPV testing at the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University from September 2014 to December 2020 to examine the distribution of different types of HPV infection. From March 2015 to September 2017, 3,014 patients with known HPV underwent colposcopy to analyze high-risk HPV infection for different cervical lesions. Meanwhile, from September 2014 to December 2020, 1,616 patients positive for HPV16/18/52/58 alone with normal TCT who underwent colposcopy in our hospital were retrospectively analyzed for the occurrence of cervical and vulvovaginal lesions, with colposcopic biopsy pathology results serving as the gold standard for statistical analysis.ResultAnalysis of 244,218 patients who had HPV tested revealed that the top 3 high-risk HPV types were HPV52, HPV58, and HPV16. Further analysis of 3,014 patients showed that 78.04% of patients referred for colposcopy had HPV16/18/52/58 alone. Among high-grade squamous intraepithelial lesions (HSIL) and cervical cancer, the most common is HPV16, followed by HPV58 and then HPV52 (p < 0.05). A total of 1,616 patients with normal TCT who were referred for colposcopy due to HPV16/18/52/58 infection were further analyzed. Based on pathological findings in lesions of HSIL and CC, HPV16 is the most common, followed by HPV58 and then HPV18 (p < 0.05). In the 1,616 patients analyzed, high-grade vulvovaginal lesions were detected, with HPV58 being the most common, followed by HPV16 and then HPV52 (p < 0.05).Conclusion1. In patients with positive HPV58 alone and normal TCT, the indications for colposcopy may be relaxed, with particular attention paid to the possibility of vulvar and vaginal lesions.2. Patients with a positive HPV type 52 alone and normal TCT may be considered for a follow-up review and, if necessary, a colposcopy.3. The development of a more suitable HPV vaccine for the Asian population, such as HPV16/18/52/58, may better protect women’s health.

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<p>An efficient method that combines the ThinPrep cytologic test with E6/E7 mRNA testing for cervical cancer screening</p>

Cited by 11 publications

References 26 publications

The diagnostic value of HPV E6/E7 mRNA test in young women with cervical squamous intraepithelial lesion: A Retrospective Analysis

The diagnostic value of HPV E6/E7 mRNA test in young women with cervical squamous intraepithelial lesion: A Retrospective Analysis

The diagnostic accuracy of TCT + HPV-DNA for cervical cancer: systematic review and meta-analysis

Implications of Persistent HPV52 and HPV58 Positivity for the Management of Cervical Lesions

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