&lt;p&gt;Bioinformatic analysis of miR-4792 regulates &lt;em&gt;Radix Tetrastigma hemsleyani&lt;/em&gt; flavone to inhibit proliferation, invasion, and induce apoptosis of A549 cells&lt;/p&gt;

Liu, Peigang; Pu, Jinbao; Zhang, Junhui; Chen, Zhilu; Wei, Kemin; Shi, Lixin

doi:10.2147/ott.s182525

Cited by 13 publications

(12 citation statements)

References 49 publications

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“…Lung cancer is the only malignant tumor for which morbidity and mortality rates have been increasing in recent years (1). In addition, lung cancer has become the leading cause of cancer-associated mortality worldwide (1).…”

Section: Discussionmentioning

confidence: 99%

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Effect of miR‑140‑5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism

et al. 2019

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Non-small cell lung cancer (NSCLC) is the most common type of lung cancer accounting for ~80% of lung cancer cases. According to novel research, numerous microRNAs (miRs) have been suggested to function as important regulators of cancer. In addition, the expression of miR-140-5p is decreased in patients with NSCLC. Therefore, it is important to further elucidate the role of miR-140-5p in NSCLC. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used in order to investigate the expression of miR-140-5p in NSCLC tissues and matched normal tissues and to determine miR-140-5p levels following transfection with mimics into A549 lung cancer cells. Targetscan software was used to predict the oncogene target of miR-140-5p. This analysis revealed that YES proto-oncogene 1 (YES1) includes a target site for miR-140-5p binding. The results revealed that YES1 is a potential target gene of miR-140-5p, and this was further confirmed by the results of luciferase reporter assays, which demonstrated that miR-140-5p directly targeted the predicted binding site in the 3'-untranslated region of YES1. Cell Counting Kit-8 (CCK-8) and flow cytometry assays were performed to determine the levels of cell viability and apoptosis. Western blot assays was performed to investigate the expression levels of YES1 and proteins associated with apoptosis in A549 cells following transfection. The results revealed that miR-140-5p expression was significantly downregulated in NSCLC tissues compared with matched normal tissues. The expression of miR-140-5p was significantly increased following transfection with miR-140-5p mimics. The results of CCK-8 and flow cytometry assays indicated that miR-140-5p inhibited proliferation and induced apoptosis of tumor cells. Western blot analysis and RT-qPCR revealed that YES1 and B-cell lymphoma 2 (Bcl-2) mRNA and protein expression levels were markedly decreased in A549 cells, while Bcl-2 associated X (Bax) and caspase-3 expression levels increased significantly following transfection with miR-140-5p mimics compared with the negative control group. In conclusion, miR-140-5p may induce apoptosis in A549 cells by targeting YES1 and regulating the expression of apoptosis-associated proteins Bcl-2, Bax and caspase-3.

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Section: Discussionmentioning

confidence: 99%

“…Lung cancer is the leading cause of cancer-associated mortality, with ~1.35 million novel cases being diagnosed each year worldwide (1). A total of ~75-80% of lung cancer cases are non-small cell lung cancer (NSCLC), with the overall 5-year survival rate of 10% (2).…”

Section: Introductionmentioning

confidence: 99%

Effect of miR‑140‑5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism

et al. 2019

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“…Sporothrix schenckii yeast induces robust activation of JNK, ERK1/2 and p38 MAPK in dendritic cells, which is related to IL-6 and TNF-α secretion. Recent study demonstrated that miR-4792 participates in the apoptotic induction of A549 cells by RTHF via MAPK pathway and determines the potential apoptotic mechanism [56]. In this study, we found that miR-4792 mimics/inhibitors did not signi cantly in uence EGFR expression at the transcription level, suggestive of translational regulation.…”

Section: Discussionmentioning

confidence: 55%

MiR-4792 regulates inflammatory responses in Cryptococcus neoformans infected microglia

Chen

Yao

Wang

et al. 2020

Preprint

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Background: Investigating the factors that influence inflammatory response of microglial cells is important to understand the pathogenesis of cryptococcal meningitis (CM). MicroRNA (miRNA) have been shown to play an important role in inducing host defenses and activating immune response in the process of microbial infection; however, the regulatory mechanisms of miRNAs in cryptococcal meningitis are poorly defined. In our previous analysis, we assessed the miRNA profiles of BV2 cells following Cryptococcus neoformans (C. neoformans) infection. In this study, we characterized the expression of miR-4792 in CM patients to further our understanding of the host response to pathogen infections. Results: miR-4792 was downregulated in BV2 cells infected with C. neoformans while its target gene EGFR was upregulated. Infected cells with up-regulated miR-4792 exhibited a trend towards decreased EGFR transcript expression, reduced MAPK signaling and a decreased secretion of inflammatory cytokines. Afterantifungal treatment in cryptococcal meningitis patients, the levels of miR-4792 in the CSF significantly increased, while the expression of EGFR significantly decreased. Conclusion: This study identified that miR-4792 and its target gene EGFR regulate the secretion of inflammatory cytokines in BV2 cells infected with C. neoformans. This furthers our knowledge of the inflammatoryresponses to fungal infections in the CNS.

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“…As a result, 162 miRNAs have been identified to display expression changes >1.2fold in RTHF-treated A549 cells. On the basis of the above research, Liu P. et al (2019) also find that miR-4792 may be involved in the inhibition of A549 cell proliferation and metastasis mediated by total flavones of TDG. Meanwhile, total flavones of TDG is to down-regulate the overexpression of miRNA in cancer, and also upregulate the expression of tumor suppressor miRNAs.…”

Section: Pharmacological Activities Anti-tumor Activitymentioning

confidence: 79%

“…As a result, 162 miRNAs have been identified to display expression changes >1.2-fold in RTHF-treated A549 cells. On the basis of the above research, Liu P. et al. (2019) also find that miR-4792 may be involved in the inhibition of A549 cell proliferation and metastasis mediated by total flavones of TDG.…”

Section: Pharmacological Activitiesmentioning

confidence: 79%

The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

Zhu

Ying

et al. 2020

Front. Pharmacol.

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Tetrastigma hemsleyanum Diels & Gilg (TDG), the family member of Vitaceae, is a traditional herbal medicine in China. The root of TDG can be immediately used after cleaning the muddy soil, and can be dehydrated for dry use. TDG is able to be collected all year round, which is commonly used in the treatment of hepatitis, infantile high fever, snake bite, etc. Based on phytochemistry, the chemical components of TDG are divided into flavonoids, phenolic acids, terpenes, steroids, polysaccharide, and other compounds, showing many pharmacological effects which include anti-tumor, antioxidation, anti-inflammatory, antipyretic, analgesic, and immunomodulatory activity, as well as other activities. Currently, TDG involves some problems of the reduction of wild resources, the backward processing methods, and storage difficulties as well as the imperfection of detection methods. Therefore, this review summarizes the literature of the past 20 years, and the purpose of this review is to summarize the recent researches on the phytochemistry, pharmacology, quality control, and clinical application of TDG. The above discussions provide new insights for the future research on TDG.

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<p>Bioinformatic analysis of miR-4792 regulates <em>Radix Tetrastigma hemsleyani</em> flavone to inhibit proliferation, invasion, and induce apoptosis of A549 cells</p>

Cited by 13 publications

References 49 publications

Effect of miR‑140‑5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism

Effect of miR‑140‑5p on the regulation of proliferation and apoptosis in NSCLC and its underlying mechanism

MiR-4792 regulates inflammatory responses in Cryptococcus neoformans infected microglia

The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

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