<p>Catalpol Enhances Neurogenesis And Inhibits Apoptosis Of New Neurons Via BDNF, But Not The BDNF/Trkb Pathway</p>

Abstract

BackgroundThe role of catalpol in brain neurogenesis and newborn neuron survival has not been previously determined in permanent middle cerebral artery occlusion (pMCAO).MethodsFifty-four rats were divided into 6 groups: pMCAO (model, n=9); sham operation (NS, n=9); catalpol treatment (5 mg/kg and 10 mg/kg subgroups, n=9 each); K252a (n=9); and K252a+catalpol 5 mg/kg (n=9) with stroke. The effects of catalpol on behavior, neurogenesis surrounding the infarction ipsilateral to pMCAO, and the expression of brain-derived neurotrophic factor (BDNF) and its receptor (TrkB) were evaluated. Vehicle or, K252a (i.p.), an inhibitor of TrkB phosphorylase.ResultsRepeated administration of catalpol reduced neurological deficits and significantly improved neurogenesis. Catalpol increased the number of newborn immature neurons, as determined by BrdU+-Nestin+ and BrdU+-Tuj-1+ staining, and downregulated cleaved caspase 3 in Tuj-1+ cells at day 7 following stroke. Moreover, catalpol increased the protein expression of Tuj-1, MAP2, and the Bcl-2/Bax ratio, as determined using Western blot. Catalpol also significantly increased brain levels of BDNF, but not TrkB, resulting in enhanced survival of newborn neurons via inhibition of apoptosis.ConclusionCatalpol may contribute to neurogenesis in infarcted brain regions and help promote the survival of newborn neurons by activating BDNF, but not BDNF/TrkB signaling.

Novel controlled and targeted releasing hydrogen sulfide system exerts combinational cerebral and myocardial protection after cardiac arrest

“…Sprague-Dawley rats were anesthetized and heparinized (0.2 mL, I.P .). Then the CA/CPR rat model was established by the previous method [ 30 ] (described in Additional file 1 ). Rats were randomized into three groups: (1) sham (n = 6), which were subjected to the same procedure as the other groups except for CA/CPR; (2) Control group (n = 6), which underwent 5 min of CA and received injection of same volume of saline; (3) DATS@MION-PEG-LF group (n = 6), which underwent 5 min of CA and received injection of DATS@MION-PEG-LF (10 mg/kg).…”

Resveratrol improved hippocampal neurogenesis following lead exposure in rats through activation of SIRT1 signaling

“…42 In addition, it has been reported that Catalpol increases neurogenesis and inhibited apoptosis of new neurons by promoting BDNF expression. 43 Interestingly, BDNF also plays an important role in adult neurogenesis. 44 Ki-67 and NeuN, important markers of neurogenesis, are essential for specific functions in the mammalian hippocampus, presumably related to cognitive function.…”

“…This is likely due to the activation of SIRT1 expression by resveratrol, which enhances neurogenesis and inhibits apoptosis of nascent neurons by promoting BDNF. 43,49 However, the hypothesis that CREB/BDNF enhances neurogenesis should be interpreted with caution. This presumption will be further verified by cell culture experiments.…”

<p>Traditional Chinese Medicine Jiuwei Zhenxin Granules in Treating Depression: An Overview</p>

“…Catalpol is one of the main chemical extracts of RG. It protects dopaminergic neurons from damage in PD, 116 enhances neurogenesis and inhibits apoptosis of new neurons by activating BDNF, 117 ameliorates hyperactive and impulsive behavior, improves spatial learning and memory, 118 improves axonal growth by regulating miR-124 after ischemia, 119 relieves neuralgia, 120 and has antidepressant effects. 121 Cinnamomum cassia (L.) J. Presl CS is in the family of camphor, it is a medium-sized tree of the camphor family, and its bark is gray-brown.…”