<p>Characterization of <em>SCCmec</em>, <em>Spa</em> Types and Multidrug Resistant of Methicillin-Resistant <em>Staphylococcus aureus</em> Isolates in Ahvaz, Iran</p>

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most pathogens associated with health care. Molecular typing methods are vital for outbreak investigations of MRSA. The aim of this study was characterization of SCCmec, spa types and multidrug resistant of methicillin-resistant Staphylococcus aureus isolates in Ahvaz, Iran. Methods: A total of 50 MRSA isolates were determined by using the phenotypic method and mecA gene. Antibiotic resistance profile and SCCmec types were screened using disc diffusion method and PCR, respectively. For spa typing of MRSA isolates, two molecular typing methods including the PCR-sequencing and high-resolution melting (HRM) analysis were used. Results: In the present study, the highest sensitivity of MRSA was to vancomycin and linezolid and the lowest to clindamycin. In the MRSA isolates, 22% were XDR and 78% were MDR. SCCmec type III was found commonly among MRSA. Based on PCR-sequencing and HRM results, 10 different spa types were identified. The spa types t037 and t030 were the most common in this study. Conclusion: This study emphasizes the spa variation among MRSA isolates, which may be considered as an important criterion when treating staphylococcal infections. Accurate and early detection of MDR, XDR, or even PDR MRSA isolates strains must be commenced by all clinical microbiology laboratories to reduce the menace of antimicrobial resistance.

Prevalence of PVL and LucED Genes in Methicillin-Resistant and Susceptible Isolates of Staphylococcus aureus Isolated from Burn Patients in Kermanshah, Iran

“…Concerning studies performed in Iran, the prevalence of MRSA ranged from 29% to 40% in non-burn patients and 60% to 90% in burn patients, which indicates a higher prevalence of MRSA strains in burn patients than in non-burn ones. Indeed, these results are in line with our findings (22)(23)(24)(25)(26).…”

“…Other studies in Iran and abroad have reported high levels of resistance in MRSA isolates to different antibiotics, including penicillin, gentamicin, and ciprofloxacin (30)(31)(32). In a study carried out by Moosavian et al (25), all MRSA isolates were sensitive to linezolid and vancomycin (25).…”

Study of biofilm formation, structure and antibiotic resistance in Staphylococcus saprophyticus strains causing urinary tract infection in women in Ahvaz, Iran

“…Existence of the mecA gene was investigated using the PCR assay, as earlier defined by Moosavian et al. [ 19 ], The S. aureus ATCC 33591 strain was used as a positive control and S. aureus ATCC 25923 strain was the negative control.…”

Antibacterial activity and synergy of antibiotics with sanguisorbigenin isolated from Sanguisorba officinalis L. against methicillin‐resistant Staphylococcus aureus

“…The resistance mechanisms to β-lactam antibiotics are associated with the presence of a penicillin-binding protein (PBP2) encoded by the mecA gene in MRSA (Moosavian et al 2020). The affinity of most β-lactam reduced due to the overexpression of mecA encoded PBP2a (Hui et al 2020).…”