&lt;p&gt;Circular RNA circ_0006282 Contributes to the Progression of Gastric Cancer by Sponging miR-155 to Upregulate the Expression of FBXO22&lt;/p&gt;

He, Yi‐Ren; Wang, Yinfeng; Liu, Liu; Liu, Shaojun; Liang, Linbo; Chen, Yinan; Zhu, Zhiqiang

doi:10.2147/ott.s228216

Cited by 32 publications

(26 citation statements)

References 35 publications

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“… 34 He et al reported that circ_0006282 level was closely linked to TNM stage, tumor size and lymph nodes motility, and promoted GC cell growth, migration and invasion by competitively binding to miR-155 and reducing FBXO22. 13 Corresponding to these previous reports, circ_0006282 was strikingly elevated in GC tissues and cells. Moreover, interference of circ_0006282 restrained the proliferation, colony formation, migration and invasion capacities of GC cells in vitro and blocked the tumorigenicity of GC in vivo.…”

Section: Discussionsupporting

confidence: 58%

“…12 Circ_0006282 originated from TCEB1 gene and played an oncogenic role in GC by modulation of miR-155/FBXO22 axis. 13 Even so, the molecular mechanism by which circ_0006282 mediating GC progression remains largely unclear. In this study, the functional roles and regulatory mechanisms of circ_0006282 in GC cell growth and metastasis were investigated.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA Circ_0006282 Promotes Cell Proliferation and Metastasis in Gastric Cancer by Regulating MicroRNA-144-5p/Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein β Axis

Ye¹,

Wang²,

Wang³

et al. 2021

CMAR

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Background Circular RNAs (circRNAs) are a class of non-coding RNAs which function as novel regulators in human cancers. In this study, we aimed to investigate the functional roles and related molecular mechanisms of circ_0006282 in gastric cancer (GC) progression. Methods Fifty-five GC patients were enrolled in this study. GC cells (AGS and HGC-27) and normal cells (GES-1) were cultured in RPMI1640 added with 10% FBS and 1% penicillin-streptomycin. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to determine the expression levels of circ_0006282, transcription elongation factor B subunit 1 (TCEB1) mRNA, miR-144-5p and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein β (YWHAB; also known as 14-3-3β). RNase R assay was used to determine the characteristic of circ_0006282. Cell Counting Kit-8 (CCK-8) assay and colony formation assay were employed for cell proliferation. Transwell assay was conducted for cell migration and invasion. Western blot assay was carried out to measure the protein levels of Cyclin D1, matrix metalloprotein 9 (MMP9) and YWHAB. Dual-luciferase reporter assay, RNA pull-down assay and RIP assay were adopted to analyze the interaction between miR-144-5p and circ_0006282 or YWHAB. Murine xenograft model assay was performed to explore the function of circ_0006282 in vivo. Results Circ_0006282 level was increased in GC tissues and cells compared to normal tissues and cells. Silencing of circ_0006282 restrained GC cell proliferation, migration and invasion. For mechanism analysis, circ_0006282 was identified to function as the sponge for miR-144-5p to positively regulate YWHAB expression in GC cells. Moreover, miR-144-5p inhibition or YWHAB overexpression effectively reversed the impacts of circ_0006282 knockdown on GC cell growth and motility. Additionally, circ_0006282 knockdown blocked tumor growth of GC in vivo. Conclusion Circ_0006282 facilitated the malignant behaviors of GC cells through circ_0006282/miR-144-5p/YWHAB axis.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Circular RNA Circ_0006282 Promotes Cell Proliferation and Metastasis in Gastric Cancer by Regulating MicroRNA-144-5p/Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein β Axis

Ye¹,

Wang²,

Wang³

et al. 2021

CMAR

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“…As circRNA has the characteristics of stability and tissue specificity, thus it may become a tumor marker and be involved in progression of gastric cancer. Present study shows that Circ_0032821 promotes gastric cancer development by activating the MEK1/ ERK1/ 2; CircMRPS35 inhibits the progression of gastric cancer; Silencing RACRACGAP1 can enhance the sensitivity to Apatinib by targeting miR‐3657; CircRNA_0005075 inhibits gastric cancer growth via miR‐431/ p53/ epithelial stromal transformation axis; circ_0006282 promotes gastric cancer growth through miR‐155/ FBXO22 axis 11‐15 …”

Section: Discussionmentioning

confidence: 72%

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Wang

et al. 2020

Clinical Laboratory Analysis

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Background To investigate whether hsa_circ_0000520 affects Herceptin resistance in gastric cancer by regulating the PI3K‐AKT signaling. Methods The expression of hsa_circ_0000520 was detected by qRT‐PCR in gastric cancer tissues and cell lines. A Herceptin‐resistant gastric cancer cell was established. PcDNA and pcDNA‐hsa_circ_0000520 were transfected into NCI‐N87R cells and treated with Herceptin at a concentration of 10 μg/mL for 24 hours. MTT tested cell proliferation, and apoptosis was measured by flow cytometry. IGF‐1 treatment was used to activate PI3K‐Akt signaling. The expression levels of related proteins were detected. Results The expression of hsa_circ_0000520 was reduced in gastric cancer tissues and cell lines, and hsa_circ_0000520 in NCI‐N87R cells was significantly lower than that of NCI‐N87 cells. Compared with the CON group, the cell viability of the Herceptin group was significantly reduced, the apoptosis rate was significantly increased, the level of Bax protein was significantly increased, and the levels of Bcl‐2, p‐PI3K, and p‐Akt protein were significantly reduced. Compared with the Herceptin + pcDNA group, the cell viability of the Herceptin + hsa_circ_0000520 group was significantly reduced, the apoptosis rate was significantly increased, the level of Bax protein was significantly increased, and the levels of p‐PI3K and p‐Akt proteins were significantly reduced. After IGF‐1 treatment, the cell viability was significantly increased, the apoptosis rate was significantly reduced, the level of Bax protein was significantly reduced, and the level of Bcl‐2 protein was significantly increased. Conclusion Hsa_circ_0000520 overexpression may reverse the Herceptin resistance of gastric cancer cells by inhibiting the PI3K‐Akt signaling pathway.

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“…Token together, SNHG14 enhanced osteosarcoma progression through modulation of miR-433-3p/FBXO22 pathway. Recently, circular RNA (circRNA) circ_0006282 was revealed to facilitate tumor progression via sponging miR-155 to increase FBXO22 expression in gastric cancer 30 . Specifically, circ_0006282 functions as a ceRNA to spong miR-155 and cause the upregulation of its target, FBXO22, resulting in enhancement of proliferation and metastasis of gastric cancer cells 30 (Fig.…”

Section: Upstream Regulators Of Fbxo22mentioning

confidence: 99%

Emerging role of FBXO22 in carcinogenesis

et al. 2020

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The F-box protein 22 (FBXO22), one of F-box proteins, has been identified to be critically involved in carcinogenesis. FBXO22 promotes proliferation in breast cancer and lung cancer, but suppresses migration and metastasis. FBXO22 exerts oncogenetic functions via promoting the ubiquitination and degradation of its substrates, including KDM4A, KDM4B, methylated p53, p21, KLF4, LKB1, Snail, CD147, Bach1, PTEN, and HDM2. FBXO22 is also regulated by several regulatory factors such as p53, miR-155, SNHG14, and circ_0006282. In this review, we summarize the regulatory factors and downstream targets of FBXO22 in cancers, discuss its functions in tumorigenesis, and further highlight the alteration of FBXO22 expression in a variety of human malignancies. Finally, we provide novel insights for future perspectives on targeting FBXO22 as a promising strategy for cancer therapy. Facts FBXO22 targets multiple substrates for ubiquitination and degradation. FBXO22 is critically involved in tumorigenesis and tumor progression. FBXO22 might be a therapeutic target for cancer treatment. Open questions Which targets of FBXO22 are pivotal for cancer development and malignant progression? Do E3 liagses regulate the protein levels of FBXO22? How the inhibitors of FBXO22 could be developed and discovered for cancer therapy?

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<p>Circular RNA circ_0006282 Contributes to the Progression of Gastric Cancer by Sponging miR-155 to Upregulate the Expression of FBXO22</p>

Cited by 32 publications

References 35 publications

Circular RNA Circ_0006282 Promotes Cell Proliferation and Metastasis in Gastric Cancer by Regulating MicroRNA-144-5p/Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein β Axis

Circular RNA Circ_0006282 Promotes Cell Proliferation and Metastasis in Gastric Cancer by Regulating MicroRNA-144-5p/Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein β Axis

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Emerging role of FBXO22 in carcinogenesis

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