&lt;p&gt;Circular RNA circEXOC6B Inhibits the Progression of Ovarian Cancer by Sponging miR-421 and Regulating RUS1 Expression&lt;/p&gt;

Wang, Zhonghai; Zhang, Wenmin; Fang, Jinchuan; Xie, Ping; Miao, Miao; Yang, Hongwen

doi:10.2147/ott.s243040

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…Consistent with our findings, circEXOC6B is also downregulated in ovarian cancer [ 31 ] and endometrial cancer [ 32 ]. Furthermore, Wang et al [ 33 ] and Zheng et al [ 34 ] revealed that circEXOC6B suppresses the proliferation, invasion, and migration of ovarian cancer cells by sponging miR-421 and miR-376c-3p, respectively. However, as a distinct mechanism from serving as a “sponge” of miRNAs, we explored a novel role of circEXOC6B in which it could bind to protein in CRC.…”

Section: Discussionmentioning

confidence: 99%

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

Wang

Lin

et al. 2022

Mol Cancer

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Background In recent years, an increasing number of studies have indicated that circular RNA plays crucial roles in regulating tumor development and chemoresistance. Using two high-throughput RNA sequence datasets, we previously found that circEXOC6B was downregulated in colon cancer. However, its role and mechanism in colorectal cancer (CRC) remained unknown. Methods Real-time quantitative PCR was used to examine the expression of circEXOC6B in CRC tissues. In vivo and in vitro functional experiments were performed to determine the suppressor role of circEXOC6B in CRC progression. RNA pull-down, mass spectrometry, RNA-binding protein immunoprecipitation, co-immunoprecipitation, fluorescence in situ hybridization, and immunofluorescence were applied to investigate the possible mechanisms connecting circEXOC6B to CRC growth and 5-fluorouracil-induced apoptosis. Chromatin immunoprecipitation, dual-luciferase assay, western blot, and immunohistochemistry were used to explore the mechanisms underlying the HIF1A regulation of RRAGB transcription. Results circEXOC6B was downregulated in CRC tissues, and its lower expression was associated with poor prognosis of patients. Functional experiments showed that circEXOC6B inhibited growth and increased the 5-fluorouracil-induced apoptosis of CRC cells in vitro and in vivo. Mechanistically, circEXOC6B inhibited the heterodimer formation of RRAGB by binding to it, thereby suppressing the mTORC1 pathway and HIF1A level. In addition, HIF1A upregulated the transcription of RRAGB by binding to its promoter region. Altogether, the results demonstrated that a HIF1A-RRAGB-mTORC1 positive feedback loop drives tumor progression in CRC, which could be interrupted by circEXOC6B. Conclusions circEXOC6B inhibits the progression of CRC and enhances the chemosensitivity of CRC cells to 5-fluorouracil by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. circEXOC6B is a possible therapeutic target for CRC treatment.

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Section: Discussionmentioning

confidence: 99%

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

Wang

Lin

et al. 2022

Mol Cancer

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“…CircRNF20 targeted the miR-487a/HIF-1α axis to motivate glycolysis and tumorigenesis in breast cancer [37]. CircEXOC6B reduced ovarian cancer cell growth by RSU1 upregulation via absorbing miR-421 [38]. CircMBOAT2 affected cell migration of colorectal cancer via sponging miR-519d-3p to promote trophinin-associated protein [39].…”

Section: Discussionmentioning

confidence: 99%

CircRNA High Mobility Group At-hook 2 regulates cell proliferation, metastasis and glycolytic metabolism of nonsmall cell lung cancer by targeting miR-331-3p to upregulate High Mobility Group At-hook 2

Zhao

Song

et al. 2022

Anti-Cancer Drugs

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Increasing circular RNAs (circRNAs) have been identified as pivotal players in nonsmall cell lung cancer (NSCLC). The study will explore the function and mechanism of circRNA High Mobility Group AT-hook 2 (circHMGA2) in NSCLC. The circHMGA2, microRNA-331-3p (miR-331-3p) and HMGA2 expression analyses were performed via quantitative real-time PCR. Cell proliferation was assessed via Cell Counting Kit-8 and colony formation assays. Transwell migration/invasion assays were used for measuring cell metastasis. Glucose consumption and lactate production were determined for glycolytic evaluation. Western blot was used to detect the protein expression of HMGA2 and glycolytic markers. Target analysis was performed by dual-luciferase reporter, RNA immunoprecipitation and RNA pull-down assays. Xenograft tumor assay in mice was conducted for the investigation of circHMGA2 in vivo. CircHMGA2 was overexpressed in NSCLC, and high circHMGA2 level might be related to NSCLC metastasis and poor prognosis. In-vitro assays suggested that NSCLC cell growth, metastasis and glycolysis were retarded by downregulation of circHMGA2. Upregulation of HMGA2 was shown to return the anticancer response of circHMGA2 knockdown in NSCLC cells. Through interacting with miR-331-3p, circHMGA2 could regulate the expression of HMGA2. In addition, circHMGA2/miR-331-3p and miR-331-3p/HMGA2 axes were affirmed in NSCLC regulation. In-vivo analysis indicated that circHMGA2 inhibition also reduced tumorigenesis and glycolysis of NSCLC via the miR-331-3p/HMGA2 axis. This study disclosed the oncogenic role of circHMGA2 and the regulatory circHMGA2/miR-331-3p/HMGA2 axis in NSCLC.

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“…In past few years, more and more works have discovered the functions of circRNA in cancer, including OC. For example, circRNA circEXOC6B suppresses OC proliferation and invasion through inhibiting miR-421 [ 13 ]. Circular RNA S-7 is upregulated in OC and promotes EMT by restraining miR-641 [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Circ_0007841 accelerates ovarian cancer development through facilitating MEX3C expression by restraining miR-151-3p activity

Huang

Liu

2021

Aging

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The critical importance of circular RNAs (circRNAs) in human cancers, including ovarian cancer, has been discovered in the recent years. However, the roles of circ_0007841 in ovarian cancer remain unknown. In the current study, it was found that circ_0007841 expression was upregulated in ovarian cancer tissues and cell lines. Upregulation of circ_0007841 in patients with ovarian cancer predicts poor prognosis. Loss-of-function experiments discovered that circ_0007841 knockdown suppressed the proliferation, migration and invasion of ovarian cancer cells in vitro and in vivo . In terms of mechanism, circ_0007841 worked as a competing endogenous RNA (ceRNA) for miR-151-3p to facilitate MEX3C expression. Restoration of MEX3C level recovered the proliferation, migration and invasion of ovarian cancer cells. In conclusion, this study demonstrated that circ_0007841/miR-151-3p/MEX3C axis exerted important oncogenic functions in ovarian cancer.

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<p>Circular RNA circEXOC6B Inhibits the Progression of Ovarian Cancer by Sponging miR-421 and Regulating RUS1 Expression</p>

Cited by 15 publications

References 31 publications

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

CircRNA High Mobility Group At-hook 2 regulates cell proliferation, metastasis and glycolytic metabolism of nonsmall cell lung cancer by targeting miR-331-3p to upregulate High Mobility Group At-hook 2

Circ_0007841 accelerates ovarian cancer development through facilitating MEX3C expression by restraining miR-151-3p activity

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