<p>Comparative Study Of Genetic Diversity, Virulence Genotype, Biofilm Formation And Antimicrobial Resistance Of Uropathogenic <em>Escherichia coli</em> (UPEC) Isolated From Nosocomial And Community Acquired Urinary Tract Infections</p>

Abstract

IntroductionEscherichia coli is a Gram-negative opportunistic human pathogen, which has aroused considerable medical interest for being involved in cases of urinary tract infection.AimCharacterize the E. coli isolated both in the hospital and in the community.MethodologyA total of 200 E. coli isolated in urine samples from hospital and community were evaluated in biofilm formation assay and hydrophobicity MATS method. Antimicrobial susceptibility was performed through agar-diffusion technique. Virulence and ESBL production genes were observed through the polymerase chain reaction amplification of papC, fimH, fliC, kpsMTII, blaTEM, blaCTX-M, blaSHV, and blaOXA.The phylogenetic classification was based on the pattern chuA and yjaA and the region TspE4.C2 by PCR Multiplex.ResultsA higher frequency of non-adherent or poorly adherent isolates was observed in the community group. Approximately 85% of the community isolates were distributed in the highest hydrophilicity group (p<0.05). The level of resistant microorganisms was present at the same level in both source (p>0.05). About 14% of the hospital isolates were positive in the ESBL phenotypic detection test (p>0.05). Among the samples, 95% presented ESBL-encoding genes. The predominant phylogenetic group was B2 (78%). Community isolates showed a higher prevalence of virulence genes fimH, papC, and kpsMTII when compared to hospital samples.ConclusionThese data confirm the worldwide trend that isolates in the community present sometimes higher levels of virulence and antimicrobial resistance.

Antimicrobial Resistance, Virulence Factor-Encoding Genes, and Biofilm-Forming Ability of Community-Associated Uropathogenic Escherichia coli in Western Saudi Arabia

“…In contrast, the substantial number of the 50 community-associated UPEC isolates examined in this study exhibited either no (84%) or a weak ability (56%) to form biofilms. Similar results were reported by De Souza et al (2019), who found that community-associated UPEC strains are less able to form biofilms than hospital-acquired UPEC strains, which reportedly show stronger biofilm-forming ability. De Souza et al (2019) hypothesized that the poor ability of community-associated UPEC to form biofilms is due to low cell hydrophobicity observed in community-associated UPEC.…”

<p>Eight-Year Surveillance of Uropathogenic <em>Escherichia coli</em> in Southwest China</p>

“…The ESBL plasmid-bearing strains are often multi-drug resistant due to the combined carrying of resistance genes to aminoglycosides, quinolones, sulfonamides, and other antibiotics, which can reproduce and proliferate among the homologous and heterologous bacteria. 22,23 For severe infections caused by ESBL-producing isolates, carbapenems are often the preferred empirical therapeutic choice. Unfortunately, carbapenem-resistant Enterobacteriaceae (CRE) is globally increasing year by year and poses an urgent public health threat.…”

Antibiotic resistance, virulence, and phylogenetic analysis of Escherichia coli strains isolated from free-living birds in human habitats

“…A third gene– kspMTII , belongs to a group of genes responsible for the synthesis of capsules. Gene coding capsules are most frequently found in isolates from community environments compared to hospital samples [ 83 ]. The relationship between resistance and virulence traits is mainly observed among clinical strains in the hospital environment [ 81 , 84 , 85 ].…”