<p>CUB Domain-Containing Protein-1 Promotes Proliferation, Migration and Invasion in Cervical Cancer Cells</p>

Abstract

Emerging evidence have revealed significant contributions of CUB domain-containing protein-1 (CDCP1) in tumorigenesis, including colon, renal, ovarian, pancreatic, prostate and breast cancers. However, the roles of CDCP1 in cervical cancer (CC) still remain elusive. Materials and Methods: Quantitative reverse transcription polymerase chain reaction, immunohistochemistry and Western blotting were used to confirm the expression of CDCP1 in CC tissues compared with matched non-tumor tissues. In vitro, gain-of-function and loss-of-function studies were used to investigate the biological function and underlying mechanism of CDCP1 in cervical carcinogenesis. Furthermore, tumor growth was evaluated using a xenogenous subcutaneously implant model of CC cells in vivo. Results: Here, we confirmed that CDCP1 was significantly increased in human CC both in mRNA and in protein levels compared to normal cervical tissues. Furthermore, we demonstrated that increased CDCP1 expression promotes proliferation, migration, invasion and mediates the epithelial-to-mesenchymal transition phenotype in HeLa and C33A cells. Also, CDCP1 knockdown reverses all the effects of enhanced CDCP1 on cell behavior in SiHa and Caski cells. Importantly, the suppressive expression of CDCP1 repressed tumor growth in a mouse xenograft model of CC. Conclusion: In summary, our current study results provide novel insights into the role of CDCP1 in CC progression. Potentially, CDCP1 might serve as a diagnostic biomarker and a novel therapeutic target for CC.

hsa_circ_0005358 suppresses cervical cancer metastasis by interacting with PTBP1 protein to destabilize CDCP1 mRNA

“…Previous studies revealed the association of CDCP1 upregulation with metastasis in various cancers, 38 , 39 including cervical cancer. 40 Consistently, in this study, we confirmed that CDCP1 functions as an oncogene to promote the metastatic behavior of cervical cancer cells. The cell phenotypes acquired through hsa_circ_0005358 overexpression or knockdown can be reversed by enforced overexpression or inhibition of CDCP1, respectively, suggesting that CDCP1 functions via hsa_circ_0005358.…”

The effect of inflammatory cytokines on the risk of hypertrophic scar: A Mendelian Randomization Study

“…Elevated levels of signal peptide-CUB-EGF-like domain-containing protein 1 (SCUBE1) were observed in adults diagnosed with acute appendicitis, indicating its potential as a novel and promising biomarker for predicting in ammation (Altuntas et al, 2023). In another study (Tokime et al, 2008), neurotrophic factors such as nerve growth factor and GDNF were suggested to be involved in the mechanism underlying the overgrowth and hypersensitivity of sensory nerves in atopic dermatitis (Huang et al, 2020). The study indicated that, along with nerve growth factor, the increased production and release of GDNF in the skin, together with an excess of IL-18, may contribute signi cantly to itching in atopic dermatitis patients (Noskovicova et al, 2018).…”

Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma

“…HJ Wright et al indicated the therapeutic potential of targeting CDCP1 cleavage subtypes, as doing so inhibits triple-negative breast cancer metastasis [ 58 ]. Lijun et al reported that the increased expression of CDCP1 promotes proliferation, migration, invasion, and EMT in cervical cancer [ 59 ]. However, the function and potential molecular mechanism of CDCP1 in glioma remain unclear.…”