&lt;p&gt;Current Understanding of Circular RNAs in Gastric Cancer&lt;/p&gt;

Tang, Xiaohuan; Zhu, Jiaming; Liu, Yuanda; Chen, Chao; Liu, Tianzhou; Liu, Jingjing

doi:10.2147/cmar.s223204

Cited by 14 publications

(17 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More studies on alternative splicing during the transcription of hsa_circ_0085576 or its coding gene ASAP1, might be carried out to illustrate the regulatory mechanism. In addition, the diagnostic performance of circRNA has been reported in gastric cancer [33], cervical cancer [34], and even acute kidney injury [35]. Our results showed hsa_circ_0085576 may serve as a prognosis marker.…”

Section: Discussionsupporting

confidence: 54%

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Liu

Zhou

Piao

et al. 2020

Aging

View full text Add to dashboard Cite

There is emerging evidence that circular RNAs (circRNAs) act as important regulators in various cancers. It is less clear, however, what role circRNA plays in the tumorigenesis and metastasis of clear cell renal cell carcinoma (ccRCC). In this study, using bioinformatics analysis and a series of experimental analysis, we characterized a novel circRNA, hsa_circ_0085576 was up-regulated in ccRCC tissues and cell lines. High hsa_circ_0085576 expression was significantly correlated with tumor size, clinical stage, and metastasis status and poorer survival. Knockdown of hsa_circ_0085576 notably inhibited cell proliferation, migration, invasion, whereas enhanced cell apoptosis of ccRCC cells, in vitro. In contrast, overexpression of hsa_circ_0085576 had the opposite effects. Moreover, hsa_circ_0085576 silencing significantly suppressed tumor growth and metastasis, whereas overexpression of hsa_circ_0085576 had the opposite effects, in vivo, Our results further showed that hsa_circ_0085576 acted as a competitive endogenous RNAs to interact with microRNA-498, to attenuate its repressive effect on target gene Yes-associated protein 1 (YAP1). Finally, functional studies revealed that inhibition of hsa_circ_0085576 suppressed cell growth and metastasis by regulating miR-498/YAP1 signaling, in ccRCC cells. Based on these findings, hsa_circ_0085576 may represent a valuable prognostic biomarker and a potential therapeutic target to curb the tumorigenesis and metastasis of ccRCC.

show abstract

Section: Discussionsupporting

confidence: 54%

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Liu

Zhou

Piao

et al. 2020

Aging

View full text Add to dashboard Cite

show abstract

“…Recently, accumulating evidence has indicated that circular RNAs (circRNAs) with covalent closed-loop structures exert a vital regulatory effect on the occurrence and development of various cancers. 4 Abnormally expressed circRNAs in GC have been found to mediate tumor progression by regulating microRNAs: 5 for example, circ-RanGAP1 accelerated tumor metastasis in GC through a miR-877-3p/VEGFA regulatory axis. 6 Circ-PVT1 enhanced the resistance of GC cells to paclitaxel via down-regulating miR-124-3p and elevating ZEB1.…”

Section: Introductionmentioning

confidence: 99%

Exosomal CircPRRX1 Enhances Doxorubicin Resistance in Gastric Cancer by Regulating MiR-3064-5p/PTPN14 Signaling

et al. 2020

View full text Add to dashboard Cite

Purpose Gastric cancer (GC) is a malignant tumor with a high mortality rate. Drug resistance is a major obstacle to GC therapy. This study aimed to investigate the role and mechanism of exosomal circPRRX1 in doxorubicin resistance in GC. Materials and Methods HGC-27 and AGS cells were exposed to different doses of doxorubicin to construct doxorubicin-resistant cell lines. Levels of circPRRX1, miR-3064-5p, and nonreceptor tyrosine phosphatase 14 (PTPN14) were detected by quantitative real-time PCR or Western blot assay. Then, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, transwell, and Western blot assays were used to explore the function of circPRRX1 in GC cells. Interactions among circPRRX1, miR-3064-5p, and PTPN14 were confirmed by dual-luciferase reporter assay. The in vivo function of circPRRX1 was analyzed in a xenograft tumor model. Results CircPRRX1 was highly expressed in doxorubicin-resistant GC cell lines. Knockdown of circPRRX1 reversed doxorubicin resistance in doxorubicin-resistant GC cells. Additionally, extracellular circPRRX1 was carried by exosomes to spread doxorubicin resistance. CircPRRX1 silencing reduced doxorubicin resistance by targeting miR-3064-5p or regulating PTPN14. In GC patients, high levels of circPRRX1 in serum exosomes were associated with poor responses to doxorubicin treatment. Moreover, depletion of circPRRX1 reduced doxorubicin resistance in vivo. Conclusion CircPRRX1 strengthened doxorubicin resistance by modulating miR-3064-5p/PTPN14 signaling and might be a therapeutic target for GC patients.

show abstract

“…With the development of high-throughput sequencing, the roles of circular RNA (circRNA) have been adequately identified in GC. 7 , 8 circRNAs are generated from precursor mRNAs (pre-mRNAs) through back-splicing. Evidence has revealed the biogenesis of circRNAs in eukaryocytes, including splicing factor, eukaryotic initiation factor, and N 6 -methyladenosine (m 6 A).…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circCUL3 Accelerates the Warburg Effect Progression of Gastric Cancer through Regulating the STAT3/HK2 Axis

Yuan

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

The Warburg effect is a significant hallmark of gastric cancer (GC), and increasing evidence emphasizes the crucial role of circular RNAs (circRNAs) in GC tumorigenesis. However, the precise molecular mechanisms by which circRNAs drive the GC Warburg effect are still elusive. The present study was designed to unveil the roles of circRNAs and the corresponding potential mechanism. High-regulated expression of circCUL3 was observed in both GC tissues and cell lines. Clinically, the high expression of circCUL3 was closely correlated with advanced clinical stage and overall survival in GC patients. Functionally, cellular experimental investigations demonstrated that circCUL3 promoted the proliferation, glucose consumption, lactate production, ATP quantity, and extracellular acidification rate (ECAR) of GC cells. In vivo , circCUL3 knockdown repressed tumor growth. Mechanistic analysis demonstrated that circCUL3 promoted signal transducer and activator of transcription (STAT)3 expression through sponging miR-515-5p; moreover, transcription factor STAT3 accelerated the transcriptional level of hexokinase 2 (HK2). In summary, the present findings provide mechanistic insights into circCUL3/miR-515-5p/STAT3/HK2 axis regulation on the GC Warburg effect, providing a novel possibility for an understanding of GC pathogenesis.

show abstract

<p>Current Understanding of Circular RNAs in Gastric Cancer</p>

Cited by 14 publications

References 106 publications

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis

Exosomal CircPRRX1 Enhances Doxorubicin Resistance in Gastric Cancer by Regulating MiR-3064-5p/PTPN14 Signaling

Circular RNA circCUL3 Accelerates the Warburg Effect Progression of Gastric Cancer through Regulating the STAT3/HK2 Axis

Contact Info

Product

Resources

About