&lt;p&gt;CytokineExplore: An Online Tool for Statistical Analysis of Cytokine Concentration Datasets&lt;/p&gt;

Al-Saleh, Abdulwahab; Azizieh, Fawaz; Dingle, Kamaludin

doi:10.2147/jir.s253255

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…In physiological state, the control of cytokines and chemokines production include an intricate of regulatory mechanism, with an inhibitory feedback and synergic actions to guarantee the balance of mediators levels, non-inflammatory status , and tissue homeostasis ( 25 , 26 ).…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Soluble Mediator Signatures of Patients With Philadelphia Chromosome-Negative Myeloproliferative Neoplasms

et al. 2021

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BackgroundEssential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF) are clonal hematological diseases classified as Philadelphia chromosome-negative myeloproliferative neoplasms (MPN). MPN pathogenesis is associated with the presence of somatic driver mutations, bone marrow (BM) niche alterations, and tumor inflammatory status. The relevance of soluble mediators in the pathogenesis of MPN led us to analyze the levels of cytokines, chemokines, and growth factors related to inflammation, angiogenesis and hematopoiesis regulation in the BM niche of MPN patients.MethodsSoluble mediator levels in BM plasma samples from 17 healthy subjects, 28 ET, 19 PV, and 16 PMF patients were determined using a multiplex assay. Soluble mediator signatures were created from categorical analyses of high mediator producers. Soluble mediator connections and the correlation between plasma levels and clinic-laboratory parameters were also analyzed.ResultsThe soluble mediator signatures of the BM niche of PV patients revealed a highly inflammatory and pro-angiogenic milieu, with increased levels of chemokines (CCL2, CCL5, CXCL8, CXCL12, CXCL10), and growth factors (GM-CSF M-CSF, HGF, IFN-γ, IL-1β, IL-6Ra, IL-12, IL-17, IL-18, TNF-α, VEGF, and VEGF-R2). ET and PMF patients presented intermediate inflammatory and pro-angiogenic profiles. Deregulation of soluble mediators was associated with some clinic-laboratory parameters of MPN patients, including vascular events, treatment status, risk stratification of disease, hemoglobin concentration, hematocrit, and red blood cell count.ConclusionsEach MPN subtype exhibits a distinct soluble mediator signature. Deregulated production of BM soluble mediators may contribute to MPN pathogenesis and BM niche modification, provides pro-tumor stimuli, and is a potential target for future therapies.

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Section: Discussionmentioning

confidence: 99%

Bone Marrow Soluble Mediator Signatures of Patients With Philadelphia Chromosome-Negative Myeloproliferative Neoplasms

et al. 2021

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“…We investigated the production of 47 cytokines in conditioned media of natural bone marrow CD71+ erythroid cells and induced CD71+ erythroid cells. Partial least squares discriminant analysis (PLS-DA) and an importance plot of variables for two groups [20] were employed to identify differences in the production of cytokines between the two groups of CD71+ erythroid cells under study. Partial Least Squares Discriminant Analysis (PLSDA) makes a graph showing the projection multi-cytokine data on only two axes/components.…”

Section: Analysis Of Cytokine Production By Natural and Induced Cd71+...mentioning

confidence: 99%

Immunoregulatory properties of erythroid nucleated cells induced from CD34+ progenitors from bone marrow

Shevchenko,

Perik-Zavodskii,

Nazarov

et al. 2023

PLoS ONE

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CD 71+ erythroid nucleated cells have pronounced immunoregulatory properties in normal and pathological conditions. Many populations of cells with immunoregulatory properties are considered candidates for cellular immunotherapy for various pathologies. This study characterized the immunoregulatory properties of CD71+ erythroid cells derived from CD34-positive bone marrow cells under the influence of growth factors that stimulate differentiation into erythroid cells. CD34-negative bone marrow cells were used to isolate CD71+ erythroid nuclear cells. The resulting cells were used to assess the phenotype, determine the mRNA spectrum of the genes responsible for the main pathways and processes of the immune response, and obtain culture supernatants for the analysis of immunoregulatory factors. It was found that CD71+ erythroid cells derived from CD34+ cells carry the main markers of erythroid cells, but differ markedly from natural bone marrow CD71+ erythroid cells. The main differences are in the presence of the CD45+ subpopulation, distribution of terminal differentiation stages, transcriptional profile, secretion of certain cytokines, and immunosuppressive activity. The properties of induced CD71+ erythroid cells are closer to the cells of extramedullary erythropoiesis foci than to natural bone marrow CD71+ erythroid cells. Thus, when cultivating CD71+ erythroid cells for clinical experimental studies, it is necessary to take into account their pronounced immunoregulatory activity.

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“…Продукция цитокинов в культуральных средах эритробластов была исследована с помощью панели Bio-Plex Pro™ Human Cytokine Screening, 48-Plex (Bio-Rad Laboratories, США). Для выявления различий между продукцией цито кинов двумя группами эритробластов были использова ны PLSD-анализ (частичный дискриминантный анализ ме тодом наименьших квадратов) и график важности пере менных при анализе двух групп, PCA (принцип главных компонент) -при анализе трёх и более групп [13]. Было показано, что точки данных, соответствующие исследуе мым типам эритробластов, перекрываются, что говорит…”

Section: анализ продукции цитокиновunclassified

Production of immunoregulatory molecules by induced erythroblasts at various stages of cell differentiation

Nazarov

Perik-Zavodskii

Кузнецова

et al. 2022

Acta biomedica scientifica

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Introduction. Bone marrow erythroblasts produce a wide range of cytokines with opposite biological effects. This may be due to a change in the spectrum of production of immunoregulatory mediators during differentiation and small qualitative and quantitative differences in the spectrum of cytokines produced at each stage of differentiation, which may be important for the regulation of hemo- and immunopoiesis. The aim. To study the spectrum of production of mediators by erythroblasts at different stages of differentiation. Methods. Erythroblasts were obtained from CD34+ bone marrow cells of healthy donors in the presence of recombinant cytokines. Phenotype assessment was performed using flow cytometry for erythroid (CD45, CD71, CD235a, CD44) and lymphoid markers (CD3, CD4, CD8, CD16, CD19). Blocking of erythroblast differentiation at different stages was carried out using specific blocking monoclonal antibodies to melanocortin receptors (MCR) of types 1, 2 and 5. Cytokine analysis in conditioned erythroblast media was performed using the Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Bio-Rad Laboratories, USA). Cytokine production was analyzed using the CytokineExplore online tool. Results. The resulting erythroblasts are divided into positive and negative populations according to the CD45 marker, carry markers of erythroid cells CD71, CD235a and do not express linear markers of lymphoid cells. In type 1 MCR blockage, polychromatophilic erythroblasts predominate, in type 2 MCR blockage, basophilic erythroblasts predominate, and in type 5 MCR blockage, orthochromatophilic erythroblasts accumulate. According to the production of cytokines, it was shown that when using any of the blocking antibodies, we obtain cells that differ qualitatively and quantitatively in a number of mediators from the initial population of induced erythroblasts. Conclusion. Thus, we have shown qualitative and quantitative differences in the production of mediators by erythroblasts depending on the stage of differentiation, which can lead to different regulatory effects.

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<p>CytokineExplore: An Online Tool for Statistical Analysis of Cytokine Concentration Datasets</p>

Cited by 6 publications

References 34 publications

Bone Marrow Soluble Mediator Signatures of Patients With Philadelphia Chromosome-Negative Myeloproliferative Neoplasms

Bone Marrow Soluble Mediator Signatures of Patients With Philadelphia Chromosome-Negative Myeloproliferative Neoplasms

Immunoregulatory properties of erythroid nucleated cells induced from CD34+ progenitors from bone marrow

Production of immunoregulatory molecules by induced erythroblasts at various stages of cell differentiation

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