<p>Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum</p>

Abstract

Purpose: The present study synthesized silver nanoparticles (AgNPs) using the aqueous extract of a traditional medicinal product consisting of an oleoresin (a combination of macromolecules of carbohydrates and proteins) exuded from the rhizome of the plant Ferula foetida (asafoetida gum) and evaluated its biological properties. Materials and Methods: The silver nanoparticles synthesized using asafoetida gum (As-AgNPs) were characterized using UV/Vis spectroscopy, fourier infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM) and EADX. The cytotoxicity and antimicrobial activity As-AgNPs were evaluated against MCF-7 cell lines and selected microbial pathogens, respectively. Results: The synthesized silver nanoparticles were crystalline in nature with a spherical shape. The average particle size was 5.6-8.6 nm. The cytotoxicity of the synthesized As-AgNPs was evaluated against MCF-7 cell lines, and the As-AgNPs were found to be effective in inhibiting the multiplication of cancer cells. The As-AgNPs exhibited significant antimicrobial activity towards E. coli, K. pneumoniae and C. albicans. The MIC of the synthesized As-AgNPs was 7.80 μg/mL for E. coli ATCC 25922, Salmonella sp. WS50-and S. typhi; 15.60 μg/mL for S. typhimurium and S. aureus WS10, and 31.20 μg/mL for K. pneumoniae and S. aureus ATCC 43300-MRSA. In addition, MIC values of 15.60 μg/mL for C. albicans ATCC8436 and 31.20 μg/mL for C. krusei ATCC6258 were obtained. Conclusion: As asafoetida is a good traditional medicine, its involvement in the synthesis of AgNPs led the silver nanoparticles to exhibit good cytotoxic and antimicrobial effects.

Ganoderma lucidum inspired silver nanoparticles and its biomedical applications with special reference to drug resistant Escherichia coli isolates from CAUTI

“…The cell viability assay is useful to get information on cell death, survival, and metabolic activities due to toxic intrusions ( Asha Rani et al, 2009 , Chaves et al, 2019 ). There are many reports that the silver nanoparticles are exhibiting cytotoxicity in a dose-dependent manner ( Devanesan et al, 2020 , Alfuraydi et al, 2019 , AlSalhi et al, 2016 ) in human tumor cell lines. In the AgNPs treated cells, decreased metabolic activity was observed, and it varied with the nature of cell types and the size of nanoparticles ( Ahmad et al, 2008 , Devanesan et al, 2018 ).…”

“…The AgNPs were synthesized according to the method described by Devanesan et al (2020) . In brief, 9 ml of 1 mM of AgNO 3 (0.042 g in 100 ml of distilled water) was taken in a sterile conical flask.…”

“…The biomedical applications of metal-based nanoparticles promote non-specific bacterial toxicity mechanisms ( Sánchez-López et al, 2020 ). Earlier workers also reported the antibacterial activity of AgNPs ( Kannan et al, 2014 , Ranjith et al, 2017 , Alfuraydi et al, 2019 , Devanesan et al, 2020 , AlSalhi et al, 2016 ). In the present research, silver nanoparticles were prepared using the ethanol extracts of G. lucidum , and its biomedical applications were traced.…”

<p>Therapeutic Potential Assessment of Green Synthesized Zinc Oxide Nanoparticles Derived from Fennel Seeds Extract</p>

“…ampicillin (10 µg), and nystatin (50 µg), respectively, by well diffusion method. 27 , 28 Following the CLSI methodology, FSE and derived ZnO NPs and antibiotics were aseptically placed on MHA plates. They were then inoculated with 1x10 8 CFU/mL of bacterial and yeast cultures.…”

“…The cytotoxicity assessment was performed using MTT, NRU assay, and morphological changes following the methods described in previous reports. [25][26][27] For this assay, MCF-7 cells were plated in 96-well culture plates (1x10 4 cells/well). The cells were exposed to concentrations of 5, 10, 25, 50, and 100 μg/mL of ZnO NPs for 24 hours.…”