&lt;p&gt;Diversity and frequency of resistance and virulence genes in &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;KPC&lt;/sub&gt; and &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;NDM&lt;/sub&gt; co-producing &lt;em&gt;Klebsiella pneumoniae&lt;/em&gt; strains from China&lt;/p&gt;

Liu, Xin; Zhang, Jie; Li, Yini; Shen, Qiuni; Jiang, Wangjie; Zhao, Kelei; He, Yancheng; Dai, Peng; Nie, Zhihao; Xu, Xiyan; Zhou, Yingshun

doi:10.2147/idr.s214960

Cited by 23 publications

(15 citation statements)

References 36 publications

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“…They also examined the rmpA gene, which contained 3.7% of the 54 strains of K. pneumoniae isolated from different clinical specimens, which is a lower percentage than the present study. In another study, Liu et al, [48] reported that rmpA and magA were the most abundant genes among the 117 isolates of K. pneumoniae, respectively, and Fu et al, [56] observed the same frequency of genes in their study.…”

Section: Discussionmentioning

confidence: 78%

“…Analysis of the detection of KPC resistance genes showed that bla KPC (71%) was ampler than bla NDM (60%) without any signi cant differences in resistant species. Liu et al, [48] stated that among the tested isolates of K. pneumoniae,bla NDM and bla KPC genes had the highest frequency, respectively. This discrepancy may be attributed to genetic variations among strains associated with human populations and antibiotic regimens.…”

Section: Discussionmentioning

confidence: 99%

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Virulence Factors, Antibiotic Resistance patterns, and Molecular Types of Clinical Isolates of Klebsiella Pneumoniae

Ahmadi

Behzadi

Ranjbar³

2021

Preprint

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Background Klebsiella pneumoniae is armed with a wide range of antibiotic resistance mechanisms which mostly challenges effective treatment. Due to this fact, the aims of the current study were to identify the clinical strains of K . pneumoniae as well as to determine their phenotypes and molecular characterization related to antimicrobial resistance and virulence genes. Methods In this investigation, specimens from a hospital and different laboratories located in Shahr-e-Qods, Tehran, Iran were collected during a period of nine-month (December 2018 to August 2019). The isolated strains of K. pneumoniae were then identified through standard microbial and biochemical assays. Additionally, disk diffusion, combined disk, modified Hodge test and PCR were performed for antibiotic resistance of the strains and virulence genes profiling, respectively. The molecular typing was accomplished by ERIC-PCR. Results Eighty-four isolates of K. pneumoniae were identified and subjected to the study. Fifty- two percent of the isolated strains of K. pneumoniae were detected as multidrug resistant (MDR) pathotypes with the highest resistance to ceftriaxone (65%) and the lowest resistance to colistin (23%). Twenty-seven (52%) out of 52 (100%) MDR pathotypes of isolated K. pneumoniae were identified as ESBL producers. According to Modified Hodge Test (MHT) results, out of 24 resistant strains of isolated K. pneumoniae to imipenem and meropenem, 15 pathotypes (62.5%) were detected as KPC producers. The gene of blaCTX (encoding carbapenemase) with 96% ranked first, while the blaKPC gene with the prevalence of 71% ranked second among ESBL producers. The aminoglycoside resistance gene of Aac6-Ib showed the highest frequency with the prevalence percentage of 90%. The virulence genes of mrkD (94%) and magA (11%) were the highest and lowest among isolates, respectively. According to ERIC-PCR results the isolated strains of K. pneumoniae were divided into four clusters in which the cluster 4 was predominant group. Conclusions The high prevalence of antibiotic resistance and virulence genes in conjunction with a significant relationship between the strains reveals a high pathogenic capacity of the isolated pathotypes of K. pneumoniae . These findings emphasize the choose of more effective antibiotic regimens for treatment of infections caused by K. pneumoniae. Keywords: Klebsiella pneumoniae , antibiotic resistance, ESBL, virulence genes, molecular typing.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Virulence Factors, Antibiotic Resistance patterns, and Molecular Types of Clinical Isolates of Klebsiella Pneumoniae

Ahmadi

Behzadi

Ranjbar³

2021

Preprint

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“…The rst reported drug-resistant bacteria carrying bla IMP-4 and bla NDM-1 simultaneously were K. pneumoniae isolated from 2012 (30). In recent years, strains harboring bla NDM gene and another type of carbapenemase genes have emerged worldwide (31) (32,33). The location of these carbapenemase genes is variable, which may be located on chromosome genes and plasmids.…”

Section: Discussionmentioning

confidence: 99%

Carbapenem-Resistant Klebsiella Pneumoniae in Southwest China: Molecular Characterizations and Risk Factors Caused by NDM and KPC Producers

Ding

Jia

et al. 2021

Preprint

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Background: Klebsiella pneumoniae is one of the most common Enterobacteriaceae. In recent years, carbapenem-resistant Klebsiella pneumoniae (CRKP) has become one of the most important carbapenem-resistant Enterobacteriaceae. CRKP are usually resistant to antibiotics. Up to this day, the emergence of carbapenemase-producing K. pneumoniae has been a challenge for treatment of clinical infection.Methods: (i) 66 non-repetitive clinical CRKP isolates were identified by matrix-assisted laser analytical ionization time-of-flight mass spectrometer (MALDI-TOF-MS) and drug sensitivity analysis was performed by Vitek2 Compact. EDTA-synergy test and mCIM / eCIM test were used to detect drug-resistant phenotypes. (ii) Carbapenemase genes, extended-spectrum β-lactamase genes (ESBLs), cephalosporinase gene (AmpC), virulence genes, integron and resistance gene cassettes were amplified by PCR. (iii) Plasmid typing was performed by plasmid conjugation assay and PCR-based replicon typing (PBRT) method. (iv) The genetic environments of KPC-2 and NDM-1 were analyzed by using overlapping PCR. (v) MLST was used to analyze the molecular epidemiological characteristics of CRKP. (vi) Risk factors of CRKP infection by logistic regression model.Results: Our study revealed that 42 of the 66 CRKP isolates obtained from patients were identified as blaKPC-2, 24 blaNDM-1-positive strains were identified (20 blaNDM-1 and 4 blaNDM-5), of which 18 were from the neonatal departments. And CRKP strains were ESBL (extended-spectrum β-lactamases) and AmpC enzymes producer, Notably, we found two CR-hvKp (carbapenem-resistant hypervirulent klebsiella pneumoniae) strains, which contains blaKPC-2 gene and other resistant genes. Two of the 42 KPC-2-producing CRKP strains were positive for transconjugants, and the plasmid typing was the IncFII type. And two NDM-producing CRKP strains tested positive for transconjugants, which belonged to the lncX3 plasmid. Analysis of the genetic environment of these two genes has revealed that the highly conserved regions (tnpA-tnpR-ISkpn8-blaKPC-2) and conserved regions (blaNDM−1-bleMBL-trpF-tat) are associated with the dissemination of KPC-2 and NDM-1. Intl1 carrying drug resistance gene cassettes were widely distributed in CRKP. According to the MLST results, a total of 13 ST types were measured in 66 CRKP strains, ST11 and ST4495 were the main ST types, and the latter was the newly discovered ST type. Hematological disease, tracheal cannula and prior use of β-lactams and β-lactamase inhibitor combination were identified as independent risk factors for CRKP infections.Conclusion: These findings manifested the need for intensive surveillance and precautions to monitor the further spread of KPC and NDM in southwest China.

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“…The presence of the acquired carbapenemase genes (bla KPC , bla NDM , bla GES , bla IMP and bla VIM ) and mcr genes in SCAc2001 was determined by PCR amplification as described previously. [13][14][15]…”

Section: Isolation and Identification Of Aeromonas Caviae Scac2001mentioning

confidence: 99%

Co-Occurrence of the blaKPC-2 and Mcr-3.3 Gene in Aeromonas caviae SCAc2001 Isolated from Patients with Diarrheal Disease

Tang¹,

Huang²,

She³

et al. 2020

IDR

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To characterize the genetic feature of a multi-drug-resistant Aeromonas caviae strain isolated from the diarrhea sample of a 45-year-old male patient with acute diarrhea. Materials and Methods: Whole-genome of the A. caviae strain SCAc2001 was sequenced via the Illumina system, followed by a series of bioinformatic analyses to describe the genetic feature. Results: The genome sequence of A. caviae SCAc2001 was assembled into 340 scaffolds (305 of them were > 1000 bp in length and 4,487,370 bp in total) with an average G+C content of 61.09%. Phylogenetic analysis showed that the A. caviae SCAc2001 strain was highly similar to the A. caviae strain R25-2 and T25-39. Resistome analysis identified that A. caviae SCAc2001 carried 13 antimicrobial resistance genes, including β-lactams (bla KPC , bla CTX-M-14 , bla TEM-1 , bla OXA-10 , bla OXA-427 , bla VEB-3 and bla MOX-6), aminoglycosides (aadA1), fluoroquinolones (aac(6ʹ)-Ib-cr), phenicol resistance (catB3), sulfonamide (sul1), trimethoprim (dfrA5) and colistin resistance (mcr-3.3).And also, A. caviae ScAc2001 carried 54 putative virulence genes including the type IV pilus, fimbria, flagellarthe, and hemolysin A encoding genes, and 12 pathogen-host interactions (PHI) genes. There were also four genomic islands and eight prophages in the genome of A. caviae ScAc2001. In addition, A. caviae SCAc2001 also carried three secondary metabolism products coding clusters including nonribosomal peptide synthetases (nrps), hserlactone and bacteriocin. Conclusion: A. caviae ScAc2001 carries many resistance genes, a variety of virulence factors, PHI genes and four genomic islands and eight prophages, which poses a severe threat to infectious diseases control strategies, diagnosis methods and clinical treatment.

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<p>Diversity and frequency of resistance and virulence genes in <em>bla</em><sub>KPC</sub> and <em>bla</em><sub>NDM</sub> co-producing <em>Klebsiella pneumoniae</em> strains from China</p>

Cited by 23 publications

References 36 publications

Virulence Factors, Antibiotic Resistance patterns, and Molecular Types of Clinical Isolates of Klebsiella Pneumoniae

Virulence Factors, Antibiotic Resistance patterns, and Molecular Types of Clinical Isolates of Klebsiella Pneumoniae

Carbapenem-Resistant Klebsiella Pneumoniae in Southwest China: Molecular Characterizations and Risk Factors Caused by NDM and KPC Producers

<p>Co-Occurrence of the <em>bla</em><sub>KPC-2</sub> and <em>Mcr-3.3</em> Gene in <em>Aeromonas caviae</em> SCAc2001 Isolated from Patients with Diarrheal Disease</p>

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