<p>Down-Regulated CMTM2 Promotes Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma</p>

Abstract

Background: Our recent study identified that human chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing family member 2 (CMTM2) was deregulated in hepatocellular carcinoma (HCC) tissues and posed as a potential tumor suppressor. However, the mechanism of CMTM2 in HCC occurrence and development has not been well elaborated. Materials and Methods: The expression of CMTM2 was knocked-down by RNA interruption in Huh-7 and SMMC7721 cells. Cell proliferation ability was detected by CCK8 test and colony formation assay. The cell invasion and migration were measured by wound healing and Transwell assay. Results: We found that the cell proliferation was significantly increased by interruption of CMTM2 expression, both in Huh-7 and SMMC7721 cells. Moreover, down-regulated CMTM2 could promote the invasion and migration ability of HCC cells through inducing the epithelial-mesenchymal transition (EMT) process. We further discovered that both the expression of CMTM2 and the EMT-associated marker E-cadherin were decreased in the same thirty cases of HCC tissues compared with the corresponding adjacent non-tumor tissues. Pearson correlation test showed that there was a significantly positive correlation between CMTM2 and E-cadherin in HCC tissues (P<0.05). Conclusion: Based on the results of cell model and HCC tissues, our study suggests that down-regulated CMTM2 promotes HCC metastasis through inducing the EMT process.

Integrated Analysis of the Expression, Prognostic Value, and Relationship with Immune Infiltration of CMTMs in Human Hepatocellular Carcinoma

“…They also found a signi cant positive correlation between CMTM2 and E-cadherin expression in HCC tissues. These results suggested that down-regulation of CMTM2 expression in HCC tissues can promote HCC metastasis by inducing EMT process [27]. Combined with our results, CMTM2 may be a potential tumor suppressor gene, and high levels of CMTM2 expression may be related to a good prognosis.…”

Comprehensive analysis of the prognostic value of the chemokine-like factor-like MARVEL transmembrane domain-containing family in gastric cancer

“…Further study provided verification that knockdown of CMTM2 in vitro was remarkably associated with downregulation of E-cadherin and β-catenin expression, and upregulation of N-cadherin, Vimentin, ZEB1, and ZEB2. Also, CMTM2 was demonstrated to be involved in the inhibition of the invasion and migratory abilities of HCC cells (Huh-7 and SMMC7721), which was realized through the suppression of epithelial-mesenchymal transition (EMT) (33). Our results showed that elevated CMTM2 expression was closely related to favorable prognostic outcomes in patients with GC in terms of OS, PFS, and PPS.…”

Inhibitory effect of CC chemokine ligand 23 (CCL23)/ transcription factor activating enhancer binding protein 4 (TFAP4) on cell proliferation, invasion and angiogenesis in hepatocellular carcinoma

“…For the cell wound healing assay [ 29 ], cells were seeded in 6-well plates (1 × 10 5 cells per well) and incubated in a complete medium. When the cells grew to 80% confluency, a wound was made in the cell layer using a sterile pipette tip.…”