<p>Downregulation of Hsa_circ_0000735 Inhibits the Proliferation, Migration, Invasion, and Glycolysis in Non-small-cell Lung Cancer by Targeting miR-940/BMPER Axis</p>

Abstract

Background: Lung cancer is the most commonly diagnosed cancer and the major cause of cancer-related deaths worldwide. The increasing studies have demonstrated that circular RNA (circRNA) was involved in the progression of various cancers, including non-smallcell lung cancer (NSCLC). This study was designed to assess the expression, roles and functional mechanisms of circ_0000735 in NSCLC. Materials and Methods: The expression levels of circ_0000735, miR-940 and bone morphogenetic protein binding endothelial cell precursor-derived regulator (BMPER) were estimated by the real-time quantitative polymerase chain reaction (RT-qPCR). The biological behaviors of NSCLC cells such as proliferation, migration and invasion were analyzed by cell counting kit-8 (CCK-8), colony-forming assays and transwell assay, respectively. Furthermore, extracellular acid ratio and lactate production were tested to assess glycolysis levels of NSCLC cells. The interaction relationship among circ_0000735, BMPER and miR-940 was analyzed by bioinformatics database and dual-luciferase reporter assay. The protein expression level of BMPER was assessed by Western blot assay. Tumorigenesis assay was established to clarify the functional roles of circ_0000735 in vivo. Results: Circ_0000735 was upregulated and significantly correlated with overall survival in patients with NSCLC. In addition, the loss-of-functional experiments revealed that knockdown of circ_0000735 repressed proliferation, migration, invasion and glycolysis of NSCLC cells and tumor growth in vivo, which was overturned by overexpression of BMPER. Similarly, overexpression of circ_0000735 enhanced proliferation, migration, invasion, and glycolysis of NSCLC cells. In addition, we also confirmed that overexpression of miR-940 impeded proliferation, migration, invasion, and glycolysis of NSCLC cells. Furthermore, overexpression of BMPER abolished si-circ_0000735 induced effects on NSCLC cells. Conclusion: Circ_0000735 regulated proliferation, migration, invasion, and glycolysis in NSCLC cells by targeting miR-940/BMPER axis.

Decreased miR-940 expression can predict a negative prognosis in early-stage nonsmoking female lung adenocarcinoma

“…Jiang et al demonstrated that miR-940 could inhibit cell invasion by targeting Snail in NSCLC (18). In addition, Huang et al reported that circ_0000735 modulated miR-940 to regulate BMPER expression (28). Indeed, this axis plays an important role in NSCLC progression.…”

Circ_0078767 Inhibits the Progression of Non-Small-Cell Lung Cancer by Regulating the GPX3 Expression by Adsorbing miR-665

“…The role of circRNAs in regulating glycolysis in cancer disease has been extensively studied in recent years [ 33 ]. For example, downregulation of circSEC31A or circ_0000735 can inhibit glycolytic metabolism in NSCLC cells [ 34 , 35 ]. In this study, knocking down circ_0078767 significantly promoted the proliferation, migration, and invasion of NSCLC cells.…”

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

“…In recent years, with the deepening of circRNA research, researchers have found that the expression of circRNA is particularly important for the progression of many diseases, so it has great potential in the diagnosis and treatment of diseases. 21 , 22 In NSCLC, many circRNAs have been identified as potential biomarkers for NSCLC, such as circ_0000735, 23 circ_102179, 24 circ_103993, 25 and circ_0020123. 26 In previous studies, circ-PITX1 was found to be highly expressed in NSCLC and was related to NSCLC progression.…”