<p>Effect of 4-Fluoro-N-(4-Sulfamoylbenzyl) Benzene Sulfonamide on Acquisition and Expression of Nicotine-Induced Behavioral Sensitization and Striatal Adenosine Levels</p>

Abstract

Introduction Behavioral sensitization is a phenomenon that develops from intermittent exposure to nicotine and other psychostimulants, which often leads to heightened locomotor activity and then relapse. Sulfonamides that act as carbonic anhydrase inhibitors have a documented role in enhancing dopaminergic tone and normalizing neuroplasticity by stabilizing glutamate release. Objective The aim of the current study was to explore synthetic sulfonamides derivative 4-fluoro-N-(4-sulfamoylbenzyl) benzene-sulfonamide (4-FBS) (with documented carbonic anhydrase inhibitory activity) on acquisition and expression of nicotine-induced behavioral sensitization. Methods In the acquisition phase, selected 5 groups of mice were exposed to saline or nicotine 0.5mg/kg intraperitoneal (i.p) for 7 consecutive days. Selected 3 groups were administered with 4-FBS 20, 40, and 60 mg/kg p.o. along with nicotine. After 3 days of the drug-free period, ie, day 11, a challenge dose of nicotine was injected to all groups except saline and locomotor activity was recorded for 30 minutes. In the expression phase, mice were exposed to saline and nicotine only 0.5 mg/kg i.p for 7 consecutive days. After 3 days of the drug-free period, ie, day 11, 4-FBS at 20, 40, and 60 mg/kg were administered to the selected groups, one hour after drug a nicotine challenge dose was administered, and locomotion was recorded. At the end of behavioral experiments, all animals were decapitated and the striatum was excised and screened for changes in adenosine levels, using HPLC-UV. Results Taken together, our findings showed that 4-FBS in all 3 doses, in both sets of experiments significantly attenuated nicotine-induced behavioral sensitization in mice. Additionally, 4-FBS at 60mg/kg significantly lowered the adenosine level in the striatum. Conclusion The behavioral and adenosine modulation is promising, and more receptors level studies are warranted to explore the exact mechanism of action of 4-FBS.

Effects of 1-methyl-1, 2, 3, 4-tetrahydroisoquinoline on a diabetic neuropathic pain model

“…Subsequently, the supernatant was filtered using a 0.45 mm filter (CNW Technologies). It was then placed for analysis in an HPLC auto-sampler ( Rehman et al, 2020a ; Rehman et al, 2020b ).…”

6-Methoxyflavone and Donepezil Behavioral Plus Neurochemical Correlates in Reversing Chronic Ethanol and Withdrawal Induced Cognitive Impairment

“…Locomotor activity was assessed in activity boxes (46 × 46 cm) 27 , 28 internally divided into four quadrants measuring (23 × 23 cm) with floor line-markings. 29 All animals were placed individually into the locomotor boxes, and activity was recorded by a video camera mounted 230 cm above the box. The number of lines crossed in 30 minutes was noted, and data were logged.…”

“…The sample was then cold centrifuged at 12,000 rpm/min (4°C) (DLAB Scientific), and the supernatant was filtered using a 0.45 mm filter (CNW technologies) before introduction into the HPLC autosampler. 29 …”

“…The elution rate was set at a flow rate of 0.5 mL/min while the column was maintained at a temperature of 35 ℃. 29 , 38 …”

“…The calibration curve was then made by plotting the peak area of dopamine, noradrenaline and vitamin C (y) against the concentration of dopamine, noradrenaline and vitamin C (x), respectively, using linear regression analysis. 29 …”