<p>Effect of RSK4 on Biological Characteristics of Gastric Cancer</p>

Abstract

Purpose: Gastric cancer is one of the most common cancers with high mortality. Emerging evidences show that ribosomal s6 kinase4 (RSK4) may be an anti-oncogene in several types of cancers, while its function in GC is still unclear. In the present study, we investigated the role of RSK4 in GC progression using MGC-803 and HGC-27 cell lines in vitro and in vivo. Methods: The expression of RSK4 in gastric cancer cells was evaluated using RT-qPCR and Western blot analysis. We transfected cells with RSK4 siRNA to reduce the expression of RSK4 and then evaluated the effect of RSK4 on cellular function. MTT and cell cycle assays were used to study its effect on cell growth. Flow cytometry was used to evaluate cell apoptosis. Wound healing and Transwell assays were performed to investigate metastasis. Stable cell lines with or without RSK4 knockdown were constructed with lentivirus and tumor-bearing mice were used to investigate the effect of RSK4 on cancer progression. Results: The results revealed that reduction of RSK4 expression inhibited cell apoptosis and promoted cell proliferation, migration, and invasion. Additionally, RSK4 knockdown promoted tumorigenesis in vivo. Conclusion: Our study demonstrated that RSK4 serves as a tumor suppressor in GC.

MiR-548d-3p Promotes Gastric Cancer by Targeting RSK4

“…Similarly, in gastric cancer, reducing the expression of RSK4 inhibits cell apoptosis, leading to enhanced cell proliferation, migration, and invasion, and promotes tumor occurrence in vivo. 1 These results indicate that RSK4 has a tumor suppressor effect in a variety of cancers. However, in lung adenocarcinoma, RSK4 is highly expressed, and is closely related to TNM stage, tumor diameter, lymph node metastasis, and distant metastasis, and is considered useful for the diagnosis of lung adenocarcinoma, as well as predicting the progression of this disease.…”

“… 1 , 31 , 32 Moreover, our previous study has shown that the expression of RSK4 has an inhibitory effect on the progression of gastric cancer. 1 However, reports on miRNAs that target RSK4 in gastric cancer are limited. The present study aimed to explore the biological functions of RSK4-targeting miRNAs in gastric cancer to provide a basis for its role in tumor therapy.…”

“… 3 The disease is usually diagnosed when tissue infiltration and distant metastasis have occurred, resulting in an average 5-year survival rate of less than 20% for patients in most parts of the world. 1 , 3 , 5 Therefore, gastric cancer is still a clinical challenge for which effective early diagnosis targets and new therapeutic targets must be detected. 1 Exploring the genes that regulate gastric cancer may assist in its early diagnosis and treatment.…”

“… 1 , 28 , 29 Moreover, some researchers recommend RSK4 as a marker for evaluating the prognosis of patients. 1 , 30 However, in some breast cancer tissues, the expression of RSK4 is higher than that in neighboring tissues, and whether RSK4 exerts a carcinogenic or a tumor suppressor effect depends on many factors. 1 , 31 , 32 Moreover, our previous study has shown that the expression of RSK4 has an inhibitory effect on the progression of gastric cancer.…”

“… 1 , 30 However, in some breast cancer tissues, the expression of RSK4 is higher than that in neighboring tissues, and whether RSK4 exerts a carcinogenic or a tumor suppressor effect depends on many factors. 1 , 31 , 32 Moreover, our previous study has shown that the expression of RSK4 has an inhibitory effect on the progression of gastric cancer. 1 However, reports on miRNAs that target RSK4 in gastric cancer are limited.…”