<p>Effects of triptolide and methotrexate nanosuspensions on left ventricular remodeling in autoimmune myocarditis rats</p>

Abstract

PurposeThis study was carried out to investigate the effects of a triptolide (TP) nanosuspension and methotrexate (MTX) nanosuspension on left ventricular remodeling and cardiac function for autoimmune myocarditis (EAM) in rats. The regulating effects on inflammatory cytokines in the peripheral serum and related mechanisms are also discussed.MethodsFirst, TP and MTX were prepared as a nanosuspension, and the EAM model was successfully established in rats with cardiac myosin. Then, the effect of TP and MTX suspensions was tested in an EAM model.ResultsResults revealed that both TP and MTX suspensions could reduce the degree of myocardial fibrosis and delay the remodeling process of the left ventricle which could further improve cardiac function. Finally, it was found that inflammatory cytokines in the peripheral serum were regulated by the nonspecific immune system and the inhibition of nuclear factor-κB signaling might have partly occurred due to this mechanism.ConclusionIn summary, this study provided a complete foundation for EAM therapy of profound clinical relevance.

Discovery and Validation of Traditional Chinese and Western Medicine Combination Antirheumatoid Arthritis Drugs Based on Machine Learning (Random Forest Model)

“…Notably, triptolide combined with Western drugs, such as methotrexate, has previously been reported to produce favorable results in past experiments [35]. However, the number of predicted combinations containing triptolide in our model was not high; it predicted triptolide combined with diclofenac (75 times), naproxen (75 times), and celecoxib (24 times) but did not predict triptolide combined with methotrexate (0 times).…”

QiShenYiQi pill for myocardial collagen metabolism and apoptosis in rats of autoimmune cardiomyopathy

“…Li et al. ( 2019 ) conducted experiments in a rat model of autoimmune myocarditis, and found that excessive collagen deposition and type I/III collagen ratio increased significantly, the left ventricular diastolic function was impaired, accompanied by increased cardiac volume and weakened elasticity and contractility function. Although moderate collagen production is a repair process under certain conditions, once the balance between collagen synthesis and degradation is broken, it results in the deposition of ECM and further deposition of collagen fibres in the interstitial and perivascular areas (Lv et al.…”

Triptolide improves myocardial fibrosis in rats through inhibition of nuclear factor kappa B and NLR family pyrin domain containing 3 inflammasome pathway

“…TPL has limited effects due to its poor solubility, toxicity and water solubility, while a newly developed water-soluble minnelide, has been recently reported to show promising activity in malignancies [ 36 - 40 ]. However, the clinical effect on myocardial fibrosis has not been reported at present, and the relevant studies are all carried out with TPL [ 11 , 17 , 41 - 43 ]. Considering that the focus of this study was to explore the mechanism of whether TPL could improve the degree of MF in MF rats by inhibiting the NF-κB pathway and the activation of NLRP3 inflammasome.…”