&lt;p&gt;Efficacy Of Line Probe Assay In Detection Of Drug-Resistant Pulmonary Tuberculosis In Comparison With GeneXpert And Phenotypic Methods In Iran And Genetic Analysis Of Isolates By MIRU-VNTR&lt;/p&gt;

Kazemian, Hossein; Kardan-Yamchi, Jalil; Bahador, Abbas; Khonsari, Shadi; Nasehi, Mahshid; Hamzehloo, Gholamreza; Vaziri, Farzam; Salehi, Mohammad Reza; Feizabadi, Mohammad M.

doi:10.2147/idr.s222905

Cited by 15 publications

(7 citation statements)

References 27 publications

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“…Line probe assay (LPA) was reported to be more effective in the detection of TB and MDR-TB, being rapid, reliable, and efficient for determining TB and the resistance pattern instrumental for early diagnosis and establishing suitable therapy. High prevalence of inhA was also signified through LPA [ 76 ].…”

Section: Resistance Issues With Antitubercular Drugsmentioning

confidence: 99%

A Saudi Arabian Public Health Perspective of Tuberculosis

Saati

Khurram

Faidah

et al. 2021

IJERPH

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Tuberculosis is a global health challenge due to its spreading potential. The Kingdom of Saudi Arabia (KSA) faces a challenge in the spread of tuberculosis from migrant workers, but the foremost threat is the huge number of pilgrims who travel to visit sacred sites of the Islamic world located in the holy cities of Makkah and Al Madina. Pilgrims visit throughout the year but especially in the months of Ramadan and Zul-Hijah. The rise of resistance in Mycobacterium tuberculosis is an established global phenomenon that makes such large congregations likely hotspots in the dissemination and spread of disease at a global level. Although very stringent and effective measures exist, the threat remains due to the ever-changing dynamics of this highly pathogenic disease. This overview primarily highlights the current public health challenges posed by this disease to the Saudi health system, which needs to be highlighted not only to the concerned authorities of KSA, but also to the concerned global quarters since the pilgrims and migrants come from all parts of the world with a majority coming from high tuberculosis-burdened countries.

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Section: Resistance Issues With Antitubercular Drugsmentioning

confidence: 99%

A Saudi Arabian Public Health Perspective of Tuberculosis

Saati

Khurram

Faidah

et al. 2021

IJERPH

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“…[3][4][5][6][7][8][9] Worldwide, an estimated 10.0 million people felt ill with an estimated 1.4 million deaths in 2019. 10 Mycobacterium tuberculosis (Mtb) is a grievous pathogenic organism, 11 infecting nearly one-third of the world's population, where drug-resistant Mtb complex strains are expected to account for one third of all deaths due to TB. 12,13 The HIV/AIDS pandemic had significantly diminished public health systems and drove the rise of multidrug-resistant (MDR) and extensive-drug resistant (XDR) TB cases.…”

Section: Introductionmentioning

confidence: 99%

Magnitude of Phenotypic and MTBDRplus Line Probe Assay First-Line Anti-Tuberculosis Drug Resistance Among Tuberculosis Patients; Northwest Ethiopia

Yigzaw¹,

Torrelles²,

Wang³

et al. 2021

IDR

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Background: Mycobacterium tuberculosis (Mtb) drug resistance is a key challenge in ending TB. Objective: The study aimed to determine anti-TB drug resistance and compare the discordance between phenotypic and genotypic drug-susceptibility testing (DST). Methods: Prospective enrollment and sputum collection from patients suspected of active pulmonary TB from May 2018 to December 2019 at the University of Gondar Hospital. Phenotypic DST study for streptomycin, isoniazid, rifampin, and ethambutol was done by MGIT 360 SIRE Kit. Genotypic resistance for isoniazid and rifampin was performed by MTBDRplus v2 line probe assay (LPA) and compared to phenotypic drug resistance. Results: A total of 376 patients, median age 32 years, and 53.7% male were enrolled. Mtb was isolated from 126 patients. 106/126 (84%) patients were newly diagnosed with TB and 20 patients with prior TB treatment. Seventy (66.0%) were susceptible to all anti-TB drugs tested. Twenty-five (19.8%) of the isolates were resistant to isoniazid, 12 (9.5%) to rifampicin and six (5%) were multidrug resistant. Among previously treated TB patients, 4 (20.0%) and 5 (25.0%) were mono-resistant and poly-resistant, respectively. The sensitivity and specificity of LPA resistance for isoniazid were 94.4% and 100%, and for rifampin was 75.0% and 100%, respectively. Conclusion: The frequency of mono-and poly-drug resistance among both newly diagnosed and previously treated TB patients was high to the rest of the nation. MTBDRplus showed excellent concordance for isoniazid and rifampin. We concluded that DST should be performed for all patients to improve management and decrease spread of drug-resistant Mtb strains in the community.

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“…Out of the 509 potential studies, 52 studies met all inclusion criteria. (1, 7–59) A PRISMA flow diagram in Figure 1 illustrates the breakdown of number of articles excluded because of the specified criterion.…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review of Mutations Associated with Isoniazid Resistance Points to Lower Diagnostic Sensitivity for Common Mutations and Increased Incidence of Uncommon Mutations in Clinical Strains of Mycobacterium tuberculosis

Valafar

2020

Preprint

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Molecular testing is rapidly becoming integral to the global tuberculosis (TB) control effort. Uncommon mechanisms of resistance can escape detection by these platforms and lead to the development of Multi-Drug Resistant (MDR) strains. This article is a systematic review of published articles that reported isoniazid (INH) resistance-conferring mutations between September-2013 and December-2019. The aims were to catalogue mutations associated with INH resistance, estimate their global prevalence and co-occurrence, and their utility in molecular diagnostics. The genes commonly associated with INH resistance, katG, inhA, fabG1, and the intergenic region oxyR-ahpC were considered in this review. In total, 52 articles were included describing 5,632 INHR clinical isolates from 31 countries. The three most frequently mutated loci continue to be katG315 (4,100), inhA-15 (786), and inhA-8 (105). However, the diagnostic value of inhA-8 is far lower than previously thought, only appearing in 25 (0.4%) INHR isolates that lacked a mutation at the first two loci. Importantly, of the four katG loci recommended by the previous systematic review for diagnostics, only katG315 was observed in our INHR isolates. This indicates continued evolution and regional differences in INH resistance. We have identified 58 loci (common to both systematic reviews) in three genomic regions as a reliable basis for molecular diagnostics. We also catalogue mutations at 49 new loci associated with INH resistance. Including all observed mutations provides a cumulative sensitivity of 85.1%. The most disconcerting is the remaining 14.9% of isolates that harbor an unknown mechanism of resistance, will escape molecular detection, and likely convert to MDR-TB, further complicating treatment. Integrating the information cataloged in this and other similar studies into current diagnostic tools is essential for combating the emergence of MDR-TB. Exclusion of this information will lead to an unnatural selection which will result in eradication of the common but propagation of the uncommon mechanisms of resistance, leading to ineffective global treatment policy and a need for region-specific regiments. Finally, the observance of many low-frequency resistance-conferring mutations point to an advantage of platforms that consider regions rather than specific loci for detection of resistance.

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<p>Efficacy Of Line Probe Assay In Detection Of Drug-Resistant Pulmonary Tuberculosis In Comparison With GeneXpert And Phenotypic Methods In Iran And Genetic Analysis Of Isolates By MIRU-VNTR</p>

Cited by 15 publications

References 27 publications

A Saudi Arabian Public Health Perspective of Tuberculosis

A Saudi Arabian Public Health Perspective of Tuberculosis

Magnitude of Phenotypic and MTBDRplus Line Probe Assay First-Line Anti-Tuberculosis Drug Resistance Among Tuberculosis Patients; Northwest Ethiopia

A Systematic Review of Mutations Associated with Isoniazid Resistance Points to Lower Diagnostic Sensitivity for Common Mutations and Increased Incidence of Uncommon Mutations in Clinical Strains of Mycobacterium tuberculosis

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