<p>Eicosapentaenoic acid’s metabolism of 15-LOX-1 promotes the expression of miR-101 thus inhibits Cox2 pathway in colon cancer</p>

Abstract

It is well known that diet Eicosapentaenoic acid (EPA) is beneficial to colon cancer (CC). However, the underlying molecular mechanisms of EPA-relating miRNAs on genesis and development of this area is still unclear. Materials and Methods: This study tries to find the function and specific role of EPA in CC through quantitative PCR (qPCR), Western blotting, immunofluorescence (IF), mass spectrometry, and immunohistochemistry (IHC) assays. By these methods, the enrichment of 15-LOX-1 metabolites of EPA, the expression of miR-101 and Cox2, and the relationship among them in CC are measured. Results: The quantity of miR-101 was obviously suppressed in CC tissues and SW480 cells. After application of miR-101 mimics in CC cell lines, the Cox2 expression was inhibited too. Next, we confirmed that EPA could increase the expression of miR-101 induced by 15-LOX-1. Finally, we tested whether EPA functions as a regulator of miR-101 via the production of resolvin E3. Conclusion: Our data demonstrate that the EPA-15-LOX-1-miR-101-Cox2 signaling pathway owns a crucial position in the pathogenesis and development of diet-related CC. These findings exert exciting meanings for presenting new therapeutic angles in CC.

Exploring miRNA Profiles in Colon Cancer: A Focus on miR101-3p, miR106a-5p, and miR326

“…The significantly diminished expression of miR-101-3p and miR-326 in tumoral tissues may indicate an inverse correlation between their levels and the presence of cancer, suggesting their potential role in tumorigenesis. Other studies show a suppression of miR-101 expression in cancer tissues [51,52] similar to our study but a shortened overall survival in patients with enhanced miR-106 [53,54] and high expression of miR-326 in patients with decreased progression-free survival [34]. Moreover, we demonstrated that between different stages of colon cancer, all three miRNAs showed decreased expression in advanced stages of colon cancer compared to earlier stages.…”

The Regulatory Effect of MicroRNA‐101‐3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway

“…Previous studies have reported that miR-101 is involved in a variety of human physiological and pathological processes [36][37][38][39][40][41]; in order to enhance the reliability of our study, the miRNA microarrays were employed to screen the differentially expressed miRNA; thereby, the miRNA-101-3p was finally chosen. Subsequently, the downstream potential target genes of miRNA-101-3p were predicted by the miRNA databases according to the sequence complementarity algorithms.…”

Fatty acids in cancer: Metabolic functions and potential treatment

“…However, 15‐LOX is thought to have an anticancer effect. The depletion of 15‐LOX‐1 can promote tumorigenesis in colon cancer 133,152 . Interestingly, the opposite was found in prostate cancer.…”

“…The depletion of 15-LOX-1 can promote tumorigenesis in colon cancer. 133,152 Interestingly, the opposite was found in prostate cancer. The results indicated that inhibition of 15-LOX-1 can induce apoptosis.…”