<p>ELR positive CXCL chemokines are highly expressed in an animal model of ulcerative colitis</p>

Abstract

Background: The presence of neutrophil-rich inflammation in colon tissues of patients with ulcerative colitis (UC) is one of the most important histological characteristics of this disease. However, the expression of CXCL chemokines governing the infiltration of neutrophils in UC has not been well elucidated. Materials and methods: In this experimental study, the UC model was induced in Wistar rats by administration of 2 mL 4% acetic acid into the large colon through the rectum. Animals were anesthetized after 48 hrs; their colon tissue samples were isolated for macroscopic and histopathological examinations. The expression of CXCL family was assessed by reverse transcription polymerase chain reaction (qRT-PCR) technique. Results: Heavy infiltration of neutrophils, coagulation necrosis, and ulcers were observed in H&E staining, which pathologically proved the UC model. qRT-PCR results showed that ELR + CXC chemokines such as CXCL6 and CXCL3 had the highest expression in the UC group, which was 49 and 28 times higher than that of the control group, respectively. In addition, other chemokines of this group including CXCL1, CXCL2, and CXCL7 had a significant increase compared to the control group (P≤0.05). However, ELR − CXC chemokines such as CXCL4, CXCL13, and CXCL16 showed a smaller upregulation, while CXCL14 chemokine showed a significant decrease compared to the control group (P≤0.05). However, the expression of CXCL9-12 and CXCL17 did not change. Conclusion: The results showed that the ELR + CXC chemokines, especially CXCL6 and CXCL3, many involved in the pathogenesis of UC; therefore, CXCL6 and CXCL3 chemokines can be used as therapeutic targets for UC, although more studies using human samples are required.

Identification of Novel Population-Specific Cell Subsets in Chinese Ulcerative Colitis Patients Using Single-Cell RNA Sequencing

“…Both of them are the risk genes and were verified upregulated in UC. 47 Type 17 immunity involved in the inflammation in the pathogenesis of ulcerative colitis has been implicated, 48,49 and type 17 immunity were derived by anticommensal response or antigen presentation in UC. 50,51 We found in the DEGs in epithelial and stromal lineages that Th17 signals were increased not only in the inflamed tissue, but also in the uninflamed control tissue, with the bacteriostatic molecule LCN2 expression.…”

SLC1A1, SLC16A9, and CNTN3 Are Potential Biomarkers for the Occurrence of Colorectal Cancer

“…In addition, CXCL3 and CXCL8 are also closely related to UC and CRA. For example, it has been found that CXCL3 [28] and CXCL8 [40] participate in the pathogenesis of UC and can be used as therapeutic targets for UC. For CRA, a study by Mclean et al showed that the inflammatory cytokine genes CXCL1, CXCL2, CXCL3, CCL20, IL8 (CXCL8), CCL23, CCL19, CCL21, and CCL5are BioMed Research International 4) and CEACAM7 (carcinoembryonic antigen-related cell adhesion molecule 7) have been found to be associated with an unfavourable prognosis in CRC [41].…”

“…Some gene biomarkers, such as mRNA and miRNAs, have been previously identified to correlate with CRC and developed as diagnostic tools to predict the occurrence, progression, and prognosis of CRC [21][22][23][24]. However, the identification of biomarker genes has only been focused on a single stage of CRC in many studies [25][26][27][28]. By considering all stages of disease progression, researchers can identify more accurate and targeted diagnostic gene biomarkers to be applied in clinical practice.…”

Assessment of the Microbiome Role in Skin Protection Against UV Irradiation Via Network Analysis

“…As it is reported, 17 ligands that bind to the CXC family of chemokines are identified; these ligands are known as CXCL1 to CXCL17. 22 The close relationship between these CXCLs is shown in Figures 3 and 4. The introduced CXCLs are linked mostly to the legionellosis, the collection of infections that are caused by Legionella penumophila.…”