&lt;p&gt;Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface&lt;/p&gt;

Ma, Shanshan; Wang, Zibin; Yu, Guoqing; Wang, Peng; Yang, Zukun; Han, Lu; Sun, Jianfei; Xia, Yang

doi:10.2147/ijn.s185122

Cited by 19 publications

(11 citation statements)

References 58 publications

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“…However, the cells on the composite scaffolds (PCL/nHA group, PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group) seemed to show better adherence with increased cell numbers ( Figure 7F ) and better cell-fiber entanglement, perhaps due to the better surface properties with increased hydrophilicity and the presence of the nanoparticles and nHA providing more cell adhesion sites. 39 Nano hydroxyapatite can induce adhesion, proliferation and differentiation of osteoblasts. Zhang et al prepared composite material loaded with nHA as scaffolds for bone regeneration, and found that the composite scaffolds could promote osteogenesis.…”

Section: Resultsmentioning

confidence: 99%

Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application

Song¹,

Zhang²,

Zhang³

et al. 2021

IJN

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Background As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect. Purpose A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering. Methods In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release. In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied. Results With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased. Moreover, the hydrophilic hydroxyl group and amino group presented in nHA and nanoparticles had improved the hydrophilicity of the composite fibers. The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor. In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation. Conclusion A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning. This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.

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Section: Resultsmentioning

confidence: 99%

Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application

Song¹,

Zhang²,

Zhang³

et al. 2021

IJN

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“…In another study which focused on bone differentiation, Karimi et al applied Baghdadite nanoparticle-coated with poly l-lactic acid (PLLA) ceramics electrospun scaffold which can improved osteogenic differentiation of adipose tissue-derived mesenchymal stem cells [1]. Also in a study which was done by Ma et al polymer electrospun scaffolds coated with hydrophilic hematite nanoparticles (αFeNPs) constructed to create a bioactive interface between the cells and scaffolds and increase the osteoinduction capacities of the scaffolds [28]. In the present study, the presence of clay and graphene nanoparticles in PAN-based scaffolds were approved with positive effects on uniformity of scaffolds and lead to bone differentiation of AD-MSCs which were seeded on nanofiber scaffolds.…”

Section: Discussionmentioning

confidence: 99%

The Comparison between the Osteogenic Differentiation Potential of Clay-Polyacrylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells

Mirakabad¹,

Hosseinzadeh²,

Abbaszadeh³

et al. 2019

Nano BioMed ENG

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Nowadays, bone repair by means of stem cells potential is considered as a new approach in regenerative medicine. Adipose-derived mesenchymal stem cells (AD-MSCs) have been investigated as a plentiful cell source with the ability of osteogenic differentiation which can play an important role in bone tissue engineering applications. Discovering proper elements in combination of scaffolds structure to stimulate osteogenesis in adipose-derived stem cells is one of the major concerns in this issue. Porous polymeric scaffolds such as polyacrylonitrile (PAN) and susceptible nanoparticles have attracted a lot of attention recently due to biodegradability and differentiation potential respectively. In the present study, clay-PAN nanocomposite (CPN) and graphene-PAN scaffold have been electrospuned separately and evaluated from the point of the osteogenic potential in AD-MSCs. The objective of this study was to determine the effect of clay and graphene nanoparticles with PAN nanofibers on the fate of viability and osteogenesis of AD-MSCs. First, isolated mesenchymal cells were characterized by flow cytometry. After cell culture on the surface of scaffolds MTT assay, scanning electron microscope (SEM) and DAPI staining were done. The scaffolds were characterized and osteogenic differentiation potential of AD-MSCs has been investigated. The results have indicated that alkaline phosphatase (ALP) activity, calcium content and collagen expression of cells which cultured on clay-PAN nanofibers were higher than cells which cultured on graphene-PAN scaffold. Taken together, these results suggest that porous nanofiber clay-PAN scaffold can enhance the osteogenic differentiation of AD-MSCs, and can be used as a new biodegradable scaffold for bone tissue engineering applications.

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“…The hydrophilicity of scaffolds is one of the most important parameters in the manufacture of scaffolds to make it possible for cells to adhere to and develop [29]. Via the contact angle test, the hydrophilicity of the scaffold was evaluated [30]. In the PCL, the water touch angle is around 118.4 • ± 2.0 • .…”

Section: Hydrophilicity Testmentioning

confidence: 99%

Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application

et al. 2021

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Recently, there has been a growing interest in research on nanofibrous scaffolds developed by electrospinning bioactive plant extracts. In this study, the extract material obtained from the medicinal plant Inula graveolens (L.) was loaded on polycaprolactone (PCL) electrospun polymeric nanofibers. The combined mixture was prepared by 5% of I. graveolens at 8% (PCL) concentration and electrospun under optimal conditions. The chemical analysis, morphology, and crystallization of polymeric nanofibers were carried out by (FT-IR) spectrometer, scanning electron microscopy (SEM), and XRD diffraction. Hydrophilicity was determined by a contact angle experiment. The strength was characterized, and the toxicity of scaffolds on the cell line of fibroblasts was finally investigated. The efficiency of nanofibers to enhance the proliferation of fibroblasts was evaluated in vitro using the optimal I. graveolens/PCL solutions. The results show that I. graveolens/PCL polymeric scaffolds exhibited dispersion in homogeneous nanofibers around 72 ± 963 nm in the ratio 70/30 (V:V), with no toxicity for cells, meaning that they can be used for biomedical applications.

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<p>Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface</p>

Cited by 19 publications

References 58 publications

Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application

Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application

The Comparison between the Osteogenic Differentiation Potential of Clay-Polyacrylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells

Development of Inula graveolens (L.) Plant Extract Electrospun/Polycaprolactone Nanofibers: A Novel Material for Biomedical Application

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