&lt;p&gt;Exosome-Delivered c-Met siRNA Could Reverse Chemoresistance to Cisplatin in Gastric Cancer&lt;/p&gt;

Zhang, Qiumo; Zhang, Haiyang; Ning, Tao; Li, Dongying; Deng, Ting; Liu, Rui; Bai, Ming; Zhu, Kegan; Li, Jialu; Fan, Qian; Ying, Guoguang; Ba, Yi

doi:10.2147/ijn.s231214

Cited by 82 publications

(39 citation statements)

References 44 publications

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“…For instance, breast tumor cells were engineered with tumor suppressor miR-134 and let-7 and the deriving EVs were shown to display potent anti-tumor effects [ 26 , 27 ]. Similarly, 293 T cells were successfully transfected with siRNAs against c-Met, or with the anti-miR214 oligonucleotide to obtain anti-tumor EVs [ 28 , 29 ]. The advantages of this method are the low cost and the highly efficient EV loading.…”

Section: Discussionmentioning

confidence: 99%

Coincubation as miR-Loading Strategy to Improve the Anti-Tumor Effect of Stem Cell-Derived EVs

et al. 2021

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Extracellular vesicles are considered a novel therapeutic tool, due to their ability to transfer their cargoes to target cells. Different strategies to directly load extracellular vesicles with RNA species have been proposed. Electroporation has been used for the loading of non-active vesicles; however, the engineering of vesicles already carrying a therapeutically active cargo is still under investigation. Here, we set up a coincubation method to increase the anti-tumor effect of extracellular vesicles isolated from human liver stem cells (HLSC-EVs). Using the coincubation protocol, vesicles were loaded with the anti-tumor miRNA-145, and their effect was evaluated on renal cancer stem cell invasion. Loaded HLSC-EVs maintained their integrity and miR transfer ability. Loaded miR-145, but not miR-145 alone, was protected by RNAse digestion, possibly due to its binding to RNA-binding proteins on HLSC-EV surface, such as Annexin A2. Moreover, miR-145 coincubated HLSC-EVs were more effective in inhibiting the invasive properties of cancer stem cells, in comparison to naïve vesicles. The protocol reported here exploits a well described property of extracellular vesicles to bind nucleic acids on their surface and protect them from degradation, in order to obtain an effective miRNA loading, thus increasing the activity of therapeutically active naïve extracellular vesicles.

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Section: Discussionmentioning

confidence: 99%

Coincubation as miR-Loading Strategy to Improve the Anti-Tumor Effect of Stem Cell-Derived EVs

et al. 2021

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“…it was reported that drug sensitivity to cisplatin in patients with refractory Gc could be restored by the mechanism of exosomal-anti-mir-214 delivery to Gc cells (121). Studies have revealed that in SGc7901/ddP cells, c-Met sirna delivered by exosomes reversed the resistance to cisplatin and increased the rate of apoptosis (122). exosomal mir-21 derived from M2 macrophage is also involved in the mechanism of chemotherapy resistance in Gc (123).…”

Section: Exosomal Ncrnas As a Novel Target Of Chemotherapy Drug Treatmentioning

confidence: 99%

Exosomal non‑coding RNAs: Novel biomarkers with emerging clinical applications in gastric cancer (Review)

et al. 2020

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Gastric cancer (Gc) is one of the most common types of malignant tumor and it demonstrates high mortality rates. The majority of cases of Gc are diagnosed at an advanced stage, which seriously endangers the health of the patient. Therefore, discovering a novel diagnostic method for Gc is a current priority. exosomes are 40 to 150-nm-diameter vesicles consisting of a lipid bilayer secreted by a variety of cells that exist in multiple different types of body fluids. Exosomes contain diverse types of active substances, including rnas, proteins and lipids, and play important roles in tumor cell communication, metastasis and neovascularization, as well as tumor growth. non-coding rnas (ncrnas) do not code proteins, and instead have roles in a variety of genetic mechanisms, such as regulating the structure, expression and stability of rnas, and modulating the translation and function of proteins. in recent years, exosomal ncrnas have become a novel focus in research. an increasing number of studies have demonstrated that exosomal ncrnas can be used in the prediction and treatment of GC. The present review briefly discusses the role of exosomal ncrnas as a potential biomarker, and summarizes important regulatory genes involved in the development and progression of Gc. Contents 1. introduction 2. Biology and characterization of exosomes 3. Multiple roles of exosomal ncrnas in Gc 4. exosomal ncrnas promote metastasis of Gc 5. exosomal ncrnas participate in the regulation of Gc angiogenesis 6. exosomal ncrnas as a novel target of chemotherapy drug treatment 7. conclusion

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“…The exosomes loaded with CRISPR/Cas9 induced apoptosis and cisplatin chemosensitivity in ovarian cancer cells [ 198 ]. An increase in apoptosis and chemosensitivity was observed in cisplatin-resistant human gastric adenocarcinoma cells through treatment with si-c-Met containing exosomes derived from human kidney epithelial cell line [ 199 ]. Normal intestinal FHC cell-derived exosomes transferred miR-128-3p into oxiplatin resistant CRC cells which induced their chemosensitivity and decreased motility [ 200 ].…”

Section: Cancer Management With Exosomesmentioning

confidence: 99%

Trends in Research on Exosomes in Cancer Progression and Anticancer Therapy

Sinha

Roy

Saha

et al. 2021

Cancers

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Exosomes, the endosome-derived bilayered extracellular nanovesicles with their contribution in many aspects of cancer biology, have become one of the prime foci of research. Exosomes derived from various cells carry cargoes similar to their originator cells and their mode of generation is different compared to other extracellular vesicles. This review has tried to cover all aspects of exosome biogenesis, including cargo, Rab-dependent and Rab-independent secretion of endosomes and exosomal internalization. The bioactive molecules of the tumor-derived exosomes, by virtue of their ubiquitous presence and small size, can migrate to distal parts and propagate oncogenic signaling and epigenetic regulation, modulate tumor microenvironment and facilitate immune escape, tumor progression and drug resistance responsible for cancer progression. Strategies improvised against tumor-derived exosomes include suppression of exosome uptake, modulation of exosomal cargo and removal of exosomes. Apart from the protumorigenic role, exosomal cargoes have been selectively manipulated for diagnosis, immune therapy, vaccine development, RNA therapy, stem cell therapy, drug delivery and reversal of chemoresistance against cancer. However, several challenges, including in-depth knowledge of exosome biogenesis and protein sorting, perfect and pure isolation of exosomes, large-scale production, better loading efficiency, and targeted delivery of exosomes, have to be confronted before the successful implementation of exosomes becomes possible for the diagnosis and therapy of cancer.

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<p>Exosome-Delivered c-Met siRNA Could Reverse Chemoresistance to Cisplatin in Gastric Cancer</p>

Cited by 82 publications

References 44 publications

Coincubation as miR-Loading Strategy to Improve the Anti-Tumor Effect of Stem Cell-Derived EVs

Coincubation as miR-Loading Strategy to Improve the Anti-Tumor Effect of Stem Cell-Derived EVs

Exosomal non‑coding RNAs: Novel biomarkers with emerging clinical applications in gastric cancer (Review)

Trends in Research on Exosomes in Cancer Progression and Anticancer Therapy

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