&lt;p&gt;First Report of OXA-181-Producing &lt;em&gt;Klebsiella pneumoniae&lt;/em&gt; in China&lt;/p&gt;

Liu, Congcong; Fang, Yinfei; Zeng, Yu; Lu, Jiayue; Sun, Qiaoling; Zhou, Hongwei; Shen, Zhangqi; Chen, Gongxiang

doi:10.2147/idr.s237793

Cited by 16 publications

(14 citation statements)

References 24 publications

(27 reference statements)

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“…in clinical K. pneumoniae and C. koseri from Croatia 152 . IncX 3 was associated with bla OXA‐181 in clinical K. pneumoniae, E. coli , or E. cloacae in studies from Angola, 76 Egypt, 73 and São Tomé and Príncipe; 75 bla NDM‐5 was carried on IncX 3 in E. coli in South Africa; 68 and similar findings in Mali showed E. coli harbored bla NDM‐5 , 131 in agreement with some Chinese studies that confirmed the existence of bla OXA‐181 or bla NDM‐5 on IncX 3 plasmids in K. pneumoniae or E. coli isolates 153–155 …”

Section: Carbapenemase‐associated Mgessupporting

confidence: 83%

Genetic support of carbapenemases: a One Health systematic review and meta‐analysis of current trends in Africa

Ragheb

Govinden

Sekyere

2021

Annals of the New York Academy of Sciences

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Antimicrobial resistance (AMR) is a public health threat globally. Carbapenems are β‐lactam antibiotics used as last‐resort agents for treating antibiotic‐resistant infections. Mobile genetic elements (MGEs) play an important role in the dissemination and expression of antimicrobial resistance genes (ARGs), including the mobilization of ARGs within and between species. The presence of MGEs around carbapenem‐hydrolyzing enzymes, called carbapenemases, in bacterial isolates in Africa is concerning. The association between MGEs and carbapenemases is described herein. Specific plasmid replicons, integrons, transposons, and insertion sequences were found flanking specific and different carbapenemases across the same and different clones and species isolated from humans, animals, and the environment. Notably, similar genetic contexts have been reported in non‐African countries, supporting the importance of MGEs in driving the intra‐ and interclonal and species transmission of carbapenemases in Africa and globally. Technical and budgetary limitations remain challenges for epidemiological analysis of carbapenemases in Africa, as studies undertaken with whole‐genome sequencing remained relatively few. Characterization of MGEs in antibiotic‐resistant infections can deepen our understanding of carbapenemase epidemiology and facilitate the control of AMR in Africa. Investment in genomic epidemiology will facilitate faster clinical interventions and containment of outbreaks.

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Section: Carbapenemase‐associated Mgessupporting

confidence: 83%

Genetic support of carbapenemases: a One Health systematic review and meta‐analysis of current trends in Africa

Ragheb

Govinden

Sekyere

2021

Annals of the New York Academy of Sciences

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Section: Discussionmentioning

confidence: 99%

“…The OXA-181-encoding gene was first reported in 2007 and was subsequently identified in several countries, including its recent detection in Ghana [ 15 , 16 , 33 ]. It is mainly found in E. coli and K. pneumoniae strains with an increasing prevalence [ 33 ]. The IncX3 plasmid pEC187_2-OXA-181 harbouring bla OXA-181 showed the same nucleotide identity as 17 other plasmids from 9 different countries (Czech Republic, Denmark, Myanmar, Lebanon, Japan, United Arab Emirates, United Kingdom, Switzerland and United States).…”

Section: Discussionmentioning

confidence: 99%

Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

Prah

Ayibieke

Mahazu

et al. 2021

Emerging Microbes & Infections

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The emergence and spread of carbapenemase-producing bacteria are serious threats to public health. We characterized two OXA-181-producing Escherichia coli isolates from pediatric patients with diarrhea from Ghana. bla OXA - 181 was localized on the self-conjugative IncX3-containing plasmid in the E. coli ST410 isolate, belonging to an emerging lineage, and an IncFIC(FII)-containing plasmid in E. coli ST940. The bla OXA-181 - qnrS1 region was found on the IS 26 composite transposon, which contained a 366-bp deletion in the region encoding the Rep A protein for the IncX3-containing plasmid. The IncFIC(FII) plasmid was novel and integrated with an approximately 39-kb IncX1 plasmid through conjugal transfer. Both plasmids clustered close to plasmids from Switzerland. To the best of our knowledge, this is the first report describing the presence of an IncX3 plasmid containing bla OXA-181 in strains closely related to the B4/H24RxC clade in Africa, suggesting its emergence and the need to strengthen antimicrobial resistance surveillance.

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“…So far, several studies have reported plasmids carrying bla OXA-181 with different replicons, such as IncX3, IncA/C, ColE, IncT, IncN, IncFIIK and ColKP3 (Villa et al, 2013;Naha et al, 2021). But the most common plasmid in China is the IncX3 type which has a similar genetic environment to others (Liu et al, 2020). The similar bla OXA-181 bearing IncX3 plasmid further highlights the role of IncX3type plasmid as an irreplaceable vector of ARGs (Santos Tufic-Garutti et al, 2022).…”

Section: Discussionmentioning

confidence: 98%

First report of Klebsiella pneumoniae co-producing OXA-181, CTX-M-55, and MCR-8 isolated from the patient with bacteremia

Qiao²,

Xu³

et al. 2022

Front. Microbiol.

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The worldwide spread of carbapenem-resistant Enterobacteriaceae (CRE) has led to a major challenge to human health. In this case, colistin is often used to treat the infection caused by CRE. However, the coexistence of genes conferring resistance to carbapenem and colistin is of great concern. In this work, we reported the coexistence of blaOXA-181, blaCTX-M-55, and mcr-8 in an ST273 Klebsiella pneumoniae isolate for the first time. The species identification was performed using MALDI-TOF MS, and the presence of various antimicrobial resistance genes (ARGs) and virulence genes were detected by PCR and whole-genome sequencing. Antimicrobial susceptibility testing showed that K. pneumoniae 5589 was resistant to aztreonam, imipenem, meropenem, ceftriaxone, cefotaxime, ceftazidime, levofloxacin, ciprofloxacin, gentamicin, piperacillin-tazobactam, cefepime, and polymyxin B, but sensitive to amikacin. S1-pulsed-field gel electrophoresis (PFGE) and Southern blotting revealed the mcr-8 gene was carried on a ~ 138 kb plasmid with a conserved structure (IS903B-ymoA-inhA-mcr-8-copR-baeS-dgkA-ampC). In addition, blaOXA-181 was found on another ~51 kb plasmid with a composite transposon flanked by insertion sequence IS26. The in vitro conjugation experiments and plasmid sequence probe indicated that the plasmid p5589-OXA-181 and the p5589-mcr-8 were conjugative, which may contribute to the propagation of ARGs. Relevant detection and investigation measures should be taken to control the prevalence of pathogens coharboring blaOXA-181, blaCTX-M-55 and mcr-8.

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<p>First Report of OXA-181-Producing <em>Klebsiella pneumoniae</em> in China</p>

Cited by 16 publications

References 24 publications

Genetic support of carbapenemases: a One Health systematic review and meta‐analysis of current trends in Africa

Genetic support of carbapenemases: a One Health systematic review and meta‐analysis of current trends in Africa

Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

First report of Klebsiella pneumoniae co-producing OXA-181, CTX-M-55, and MCR-8 isolated from the patient with bacteremia

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