<p>Gold quantum dots impair the tumorigenic potential of glioma stem-like cells via &beta;-catenin downregulation in vitro</p>

Abstract

Background: Over the past several decades, the incidence of solid cancers has rapidly increased worldwide. Successful removal of tumor-initiating cells within tumors is essential in the field of cancer therapeutics to improve patient disease-free survival rates. The biocompatible multivarient-sized gold nanoparticles (MVS-GNPs) from quantum dots (QDs, ,10 nm) to nanosized (up to 50 nm) particles have vast applications in various biomedical areas including cancer treatment. The role of MVS-GNPs for inhibition of tumorigenic potential and stemness of glioma was investigated in this study. Methods: Herein, MVS-GNPs synthesized and characterized by means of X-ray diffraction pattern (XRD) and transmission electron microscopy (TEM) techniques. Afterwards, interaction of these GNPs with glioma stem-cell like cells along with cancer cells were evaluated by MTT, cell motility, self-renewal assays and biostatistics was also applied. Results: Among these GNPs, G-QDs contributed to reduce metastatic events and spheroid cell growth, potentially blocking the self-renewal ability of these cells. This study also uncovers the previously unknown role of the inhibition of CTNNB1 signaling as a novel candidate to decrease the tumorigenesis of glioma spheroids and subsequent spheroid growth. The accurate and precise biostatistics results were obtained at quantify level. Conclusion: In summary, G-QDs may exhibit possible contribution on suppressing the growth of tumor-initiating cells. These data reveal a unique therapeutic approach for the elimination of residual resistant stem-like cells during cancer treatment.

Zinc oxide nanoparticles using plant Lawsonia inermis and their mosquitocidal, antimicrobial, anticancer applications showing moderate side effects

“…Gold quantum dots shown to impair the tumorigenic potential of glioma stem-like cells via β-catenin downregulation. Zinc oxide quantum dots were demonstrated as Multifunctional candidates for arresting C2C12 cancer cells and their role towards caspase 3 and 7 genes 53 . ZnO NPs also acted as anticancer agents with minimum dosage as confirmed by the IC 50 values.…”

Cold Atmospheric Plasma and Gold Quantum Dots Exert Dual Cytotoxicity Mediated by the Cell Receptor-Activated Apoptotic Pathway in Glioblastoma Cells

“…On the basis of these studies, we examined the effect of the AuQDs we synthesized in a combination with plasma treatment. We have already reported the specificity of AuQDs in glioblastoma cells [1]; therefore, we further investigated whether AuQDs improve the efficiency of plasma treatment against glioblastoma cell progression. To this end, we tested the effect of AuQDs combined with soft jet plasma.…”

“…For measurement of cellular uptake, the cells were exposed to AuQDs, washed twice with PBS, trypsinized, centrifuged at 1000 rpm for 3 min, and further resuspended in PBS. The side scattering parameter was used to measure the intracellular NP uptake of the cells, as described previously [1].…”

“…Owing to their advantages, AuQDs have been utilized for biomedical applications, including cancer therapy. In our earlier studies, we observed that AuQDs suppress cancer invasiveness and stemness, thus enhancing the anti-tumorigenic effect of drugs [1]. Nevertheless, in general, the usage of AuQDs for cancer treatment remains challenging owing to the limited uptake of nanoparticles through the cell membrane.…”

“…For instance, He et al reported that non-thermal plasma can temporarily increase cell membrane permeability to enhance endocytosis to uptake the gold nanoparticles, thus producing synergistic cytotoxicity to the target cancer cells [12]. The cold plasma-assisted nanoparticle-enhanced uptake can be due to enhanced endocytosis and trafficking to the lysosomal compartment as well as temporarily increased membrane permeability (pore formation or leaky membrane or passive diffusion) due to plasma treatment [1,4,12]. Our previous study also showcased a novel strategy of using cold plasma and PEG-coated gold nanoparticles to inhibit the PI3K/AKT signaling pathway, thus preventing the epithelial-mesenchymal transition and development of tumor [13].…”