<p>Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from <em>Momordica charantia</em></p>

Abstract

Background: In the emerging field of nanotechnology, copper oxide (CuO) nanomaterials are considered to be one of the most important transition metal oxides owing to its fascinating properties. Its synthesis from green chemistry principles is gaining importance as next-generation antibiotics due to its simplicity, eco-friendliness, and cost-effectiveness. In the present study, CuO nanorods (CuO NRs) were synthesized from the aqueous fruit extract of Momordica charantia and characterized using different analytical techniques. Further, the biomedical therapeutic potential was evaluated against multi-drug resistant microbial strains. Materials and Methods: To synthesize CuO NRs, 0.1M of CuSO 4 .5H 2 O solution was added to aqueous extract of Momordica charantia in a 1:3 (v/v) ratio (pH=11) and heated at 50°C followed by washing and drying. The synthesized CuO NRs were subjected to characterization using different analytical techniques such as UV visible spectroscopy, zeta sizer equipped with zeta potential, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) equipped with energydispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). Further, the application as a biomedical therapeutic potential was evaluated in vitro using well diffusion method against eleven multidrug-resistant clinical bacterial strains, a fungus-Trichophyton rubrum and in ovo against the R 2 B virus using haemagglutination (HA) test. Results: Characterization was preliminarily done by the spectral study that confirms the absorbance band at 245nm. FTIR analysis at 628 cm −1 peak identified copper oxide vibration. SEM analysis revealed agglomerated particle clusters. However, with TEM clear nanorods of average diameter of 61.48 ± 2 nm were observed. EDAX confirmed CuO formation while XRD showed a typical monoclinic structure with 6 nm crystallite size. Biological screening of CuO NRs showed significant results against both in vitro and in ovo methods. Significant inhibitory activity (p<0.0001) was noted against most of the resistant human pathogenic strains including both Gram-positive and Gram-negative bacteria. The highest efficacy was observed against Bacillus cereus with a 31.66 mm zone of inhibition. Besides, the therapeutic potential of CuO NRs against Corynebacterium xerosis, Streptococcus viridians and R 2 B strain of Newcastle disease is reported for the first time.Conclusion: Based on the present results, it could be expected that green synthesized CuO NRs would find potential applications in the field of nanomedicine.

Study of antimicrobial activity of Unani poly herbal toothpaste “Sunoon Zard”

“…Natural plant products are an important source to control bacterial pathogens. Therefore, in the present study, a poly-herbal tooth powder was redesigned to toothpaste form, it was standardized as per the guidelines [ 15 , 16 , 17 ] and evaluated for antibacterial activity which has showed significant results.…”

CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains

“…For the preparation of biogenic CuO nanomaterial our previous protocol was followed [ 16 ]. In order to synthesize CuO, a 0.1 M solution of CuSO 4 .5H 2 O was added to an aqueous extract of MC in a ratio of 1:3 ( v / v ) (pH 11) and was heated to 50 °C, followed by a washing and drying process.…”

“…To address all of the above issues, the present study proposes a simplified, highly versatile, and efficient method that can be employed for the design of stable nanocomposites. Previous studies have shown that CuO nanomaterial synthesized from Momordica charantia (MC) exhibits a thin layer of phytochemicals present in aqueous extracts that lead to agglomeration and the formation of unstable particles [ 16 ]. A novel method compiling green synthesized CuO nanomaterial from MC and phospholipids extracted from egg yolk in combination with glycerol has been presented.…”

Endophytic Nanotechnology: An Approach to Study Scope and Potential Applications

“…CuO-NPs were biosynthesized by the actinomycetes and enhanced antimicrobial activity was reported by Nabila and Kannabiran (2018) against some bacterial pathogens. Similarly, Qamar et al (2020) synthesized CuO-NPs from Momordica charantia plants with improved antibacterial activity against different bacterial pathogens such as Bacillus cereus , Corynebacterium xerosis , and Streptococcus viridians.…”