&lt;p&gt;Green Synthesis of Silver Nanoparticles Using Extract of &lt;em&gt;Jasminum officinal&lt;/em&gt; L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines&lt;/p&gt;

El‐Hawary, Seham S.; El-Hefnawy, Hala M; Mokhtar, Fatma Alzahraa; Sobeh, Mansour; Mostafa, Eman S.; Raey, Mohamed A. El

doi:10.2147/ijn.s269880

Cited by 39 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 Because of their unique chemical properties, NPs fabricated by a flowermediated procedure are more efficient than those synthesized using other biological materials. 11,12 The flowers of many plants contain diverse secondary compounds that function as reducing and stabilizing agents and as precursor molecules involved in the formation of NPs. 13 Numerous studies have reported the production or synthesis of AgNPs by microwave-assisted, phase transfer, electrochemical, and photochemical methods, but these synthesis steps require toxic chemicals.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Devanesan¹,

AlSalhi²

2021

IJN

113

View full text Add to dashboard Cite

Background: Abelmoschus esculentus (L.) Moench, an economically important malvaceous vegetable crop popularly known as okra, is used in various culinary preparations and is rich in vitamins, minerals, and nutrients. The biological properties of okra flowers in relation to nanoparticle synthesis have not yet been reported. Materials and Methods: In the current study, silver nanoparticles (AgNPs) were synthesized using extracts of the flowers of A. esculentus. The characteristics of the AgNPs were studied using a UV-vis spectrometer, Fourier transmission infrared spectrophotometer (FTIR), X-ray diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive X-ray spectrometer (EDX). Antibacterial activity screening was performed using the agar well diffusion method, and cytotoxicity and cell viability studies were conducted using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The synthesized AgNPs were spherical and ranged in size from 5.52 to 31.96 nm, with an average size of 16.19 nm, as determined by UV-vis spectroscopy, FTIR, XRD, TEM and EDX. A. esculentus flower extract-mediated silver nanoparticles (AME-AgNPs) exhibited excellent activities in vitro studies, particularly in vitro cytotoxic and antiproliferative studies against cancer cell lines, such as the TERT-4 and A-549 cell lines. The antibacterial effects on the Gram-positive pathogens Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes and the Gram-negative pathogens Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium and Shigella sonnei were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values varied with the bacterial strain. The IC 50 values of the synthesized NPs for the tested cell lines were close to that of a standard drug. Conclusion:Compared to other NPs the NPs synthesized in this study were smaller in size and exhibited a higher level of antibacterial activity, cytotoxicity and apoptosis at minimal concentrations, and this is the first study on okra flower-induced anticancer and antimicrobial activities.

show abstract

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Devanesan¹,

AlSalhi²

2021

IJN

113

View full text Add to dashboard Cite

show abstract

“…The surface charge of biogenic AgNPs has been observed to be −10.8 mV ( Figure 3D ). ζ-potential measures AgNPs stability by investigating the surface charge potential in aqueous suspensions ( Elhawary et al, 2020 ). A negative charge on the surface of biogenic AgNPs indicates high stability of AgNPs ( Römer et al, 2011 ).…”

Section: Resultsmentioning

confidence: 99%

Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro

et al. 2022

View full text Add to dashboard Cite

Bioactive compounds of medicinal plants present as natural ingredients provide health benefits beyond the basic nutritional value of these products. However, the availability of bioactive compounds in the current natural sources is limited. Hence, the induction of bioactive compound production from medicinal plants through nanoparticles (NPs) might play a vital role in industrially important medicinal compounds. Therefore, this study aimed to synthesize silver nanoparticles (AgNPs) biologically and to investigate their effect on phytochemical compound production from the callus of Juniperus procera. AgNPs were synthesized biologically using aqueous leaf extract of Phoenix dactylifera, which acted as a reducing and capping agent, and silver nitrate solution. The formation of AgNPs has been confirmed through different analytical techniques such as UV-Visible spectroscopy (UV), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and scanning electron microscope (SEM). The impact of different concentrations (0.0, 5, 20, and 50 mg/L) of AgNPs on enzymatic and non-enzymatic antioxidants of the callus of J. procera was investigated. The obtained results showed a significant effect of AgNPs on biomass accumulation and non-enzymatic antioxidants (phenol, tannin, and flavonoid content). Additionally, total protein content and superoxide dismutase (SOD) activity were increased in response to AgNPs. Furthermore, bioactive compounds like gallic acid, tannic acid, coumarin, hesperidin, rutin, quercetin, and ferruginol were chromatographically separated and quantified using high-performance liquid chromatography (HPLC) with reference standards. These compounds were increased significantly in response to AgNPs treatments. We concluded that AgNPs could be a promising elicitor for improving the production of phytochemical compounds in medicinal plants. This work can serve asa good model for improving the production of bioactive compounds from medicinal plants in vitro. This molecular investigation should be done to understand better the metabolic mechanism leading to bioactive compound production scaling.

show abstract

“…It is based on the ability of viable cells to incorporate and bind the supravital dye neutral red in lysosomes [ 112 ]. Neutral red uptake has been employed to evaluate the toxicity induced by AgNPs synthesized by a green route in bladder (5637) and breast (MCF-7) cancer cell lines [ 113 ]. One more common method to determine cytotoxicity measures the activity of cytoplasmic enzymes released by damaged cells.…”

Section: Cancer Evaluation Modelsmentioning

confidence: 99%

Green Metallic Nanoparticles for Cancer Therapy: Evaluation Models and Cancer Applications

et al. 2021

View full text Add to dashboard Cite

Metal-based nanoparticles are widely used to deliver bioactive molecules and drugs to improve cancer therapy. Several research works have highlighted the synthesis of gold and silver nanoparticles by green chemistry, using biological entities to minimize the use of solvents and control their physicochemical and biological properties. Recent advances in evaluating the anticancer effect of green biogenic Au and Ag nanoparticles are mainly focused on the use of conventional 2D cell culture and in vivo murine models that allow determination of the half-maximal inhibitory concentration, a critical parameter to move forward clinical trials. However, the interaction between nanoparticles and the tumor microenvironment is not yet fully understood. Therefore, it is necessary to develop more human-like evaluation models or to improve the existing ones for a better understanding of the molecular bases of cancer. This review provides recent advances in biosynthesized Au and Ag nanoparticles for seven of the most common and relevant cancers and their biological assessment. In addition, it provides a general idea of the in silico, in vitro, ex vivo, and in vivo models used for the anticancer evaluation of green biogenic metal-based nanoparticles.

show abstract

<p>Green Synthesis of Silver Nanoparticles Using Extract of <em>Jasminum officinal</em> L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines</p>

Cited by 39 publications

References 58 publications

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Biogenic silver nanoparticles improve bioactive compounds in medicinal plant Juniperus procera in vitro

Green Metallic Nanoparticles for Cancer Therapy: Evaluation Models and Cancer Applications

Contact Info

Product

Resources

About