<p>Green Synthesized BSA-Coated Selenium Nanoparticles Inhibit Bacterial Growth While Promoting Mammalian Cell Growth</p>

Abstract

Background: Selenium is an essential trace element that is critical for many biological processes. Selenium nanoparticles (SeNPs) have shown more promise than other forms of selenium due to their low cytotoxicity and high bioavailability. Methods: In this work, a one-step method was demonstrated for fabricating bovine serum albumin (BSA) stabilized SeNPs using ascorbic acid as the reductant. Human dermal fibroblasts were used to assess mammalian cytotoxicity, and Staphylococcus aureus and Escherichia coli were used to assess antibacterial performance. Results: These SeNPs demonstrated increased fibroblast growth and reduced Staphylococcus aureus growth with a fibroblast IC 50 value (>681 μg/mL) 1 order of magnitude higher than that for bacteria at day 1. Conclusion: This study demonstrated the promise of this synthesis process in achieving controllable selenium nanoparticle sizes without the use of strong basic solvents for improved antibacterial properties.

Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure

“…The choice of reducing and stabilizing agents as well as their concentrations are crucial from the point of particle size and morphology. In our previous work (Stevanović et al, 2015 ) we have already determined the optimal ratio between ascorbic acid and sodium selenite, which was also confirmed in a paper, recently published by Chung et al ( 2020 ). BSA and chitosan are selected as models of stabilizing agents since they represent different types of macromolecules.…”

“…Albumins are generally the most abundant globular proteins in serum and provide good biocompatibility while chitosan is a cationic, linear polysaccharide with documented antibacterial properties. Their application in the stabilization of SeNPs was already reported in many papers (Valueva et al, 2007;Chen et al, 2015;Lara et al, 2018;Filipović et al, 2019;Chung et al, 2020;Shi et al, 2020). The third formulation of SeNPs is designed to obtain nanoparticles without using any macromolecule as a stabilizing agent while not compromising parameters such as crystallinity, size, and morphology.…”

Green Synthesis of Selenium and Tellurium Nanoparticles: Current Trends, Biological Properties and Biomedical Applications

“…Triangular, spherical, and rod-like Se nanostructures were also efficiently fabricated in vitro using E. cloacae glutathione reductase (GorA) in both crude extracts and purified protein. Similar studies investigated biomolecules involved in mediating the reduction of selenium oxyanions to elemental selenium or SeNPs, such as glutathione (GSH) [140,156,161], glutathione reductase [162], proteins [75,163], thioredoxin reductase [162,164], SerABC reductase [165], fumarate reductase [140,141], NADH-dependent enzymes [166], NADH flavin oxidoreductase [84,166], membrane-bound SrdBCA amino acid sequence [167], DMSO reductase family of molybdoproteins [168], sulfite reductase [169], hydrogenase I [170], nitrite reductase [171], chromate selenite reductase flavoenzyme (CsrF) [75], and other enzymes and biosurfactants [172,173].…”

Selenium Nanoparticles as a Natural Antioxidant and Metabolic Regulator in Aquaculture: A Review

“…In the chemical technique, nano Se particles require different reductants using Se precursor salts. In this method, sodium selenite and ascorbic acid are used to produce Se nanoparticles which can be observed in a light orange color [ 29 ]. Ascorbic acid is also known for its functionality as antioxidative and immunostimulation roles.…”