<p>Hollow Prussian Blue Nanospheres for Photothermal/Chemo-Synergistic Therapy</p>

Abstract

Background: The integration of NIR photothermal therapy and chemotherapy is considered as a promising technique for future cancer therapy. Hollow Prussian nanospheres have attracted much attention due to excellent near-infrared photothermal conversion effect and drug-loading capability within an empty cavity. However, to date, the hollow Prussian nanospheres have been prepared by a complex procedure or in organic media, and their shell thickness and size cannot be controlled. Thus, a simple and controllable route is highly desirable to synthesize hollow Prussian nanospheres with controllable parameters. Materials and Methods: Here, in our designed synthesis route, the traditional FeCl 3 precursor was replaced with Fe 2 O 3 nanospheres, and then the Prussian blue (PB) nanoparticles were engineered into hollow-structured PB (HPB) nanospheres through an interface reaction, where the Fe 2 O 3 colloidal template provides Fe 3+ ions. The reaction mechanism and control factors of HPB nanospheres were systematically investigated. Both in vitro and in vivo biological effects of the as-synthesized HPB nanospheres were evaluated in detail. Results: Through systematical experiments, a solvent-mediated interface reaction mechanism was put forward, and the parameters of HPB nanospheres could be easily adjusted by growth time and template size under optimal water and ethanol ratio. The in vitro tests show the rapid and remarkable photothermal effects of the as-prepared HPB nanospheres under NIR laser irradiation (808 nm). Meanwhile, HPB nanospheres also demonstrated a high DOX loading capacity of 440 mg g −1 as a drug carrier, and the release of the drug can be regulated by the heat from PB shell under the exposure of an NIR laser. The in vivo experiments confirmed the outstanding performance of HPB nanospheres in photothermal/ chemo-synergistic therapy of cancer. Conclusion: A solvent-mediated template route was developed to synthesize hollow Prussian blue (HPB) nanospheres in a simple and controllable way. The in vitro and in vivo results demonstrate the as-synthesized HPB nanospheres as a promising candidate due to their low toxicity and high efficiency for cancer therapy.

Manganese-Based Prussian Blue Nanocatalysts Suppress Non-Small Cell Lung Cancer Growth and Metastasis via Photothermal and Chemodynamic Therapy

“…PB is well known as a safe photothermal agent in the treatment of tumor ( Fu et al, 2012 ; Lu et al, 2020 ). In our study, we fabricated Mn-based PB nanocatalysts to acquire enhanced synergetic effect of PTT and CDT under the guidance of MRI.…”

Synthesis of Prussian Blue Nanoparticles and Their Antibacterial, Antiinflammation and Antitumor Applications

“…Wang et al synthesized cubic, spherical, different sizes (~30–200 nm) and positively charged PBNPs by changing the rate of iron ions and adjusting the reaction time ( Figure 3 A) [ 15 ]. Lu et al prepared HPB nanospheres, which solved the problem that the shell thickness and size of PBNPs could not be controlled; they also studied in vitro and in vivo biological effects in detail [ 29 ]. Zhang et al synthesized HPBZs with internal cavities through a simple, Bi 3+ -assisted, template-free synthesis strategy ( Figure 3 B) [ 30 ].…”

“…Because of their unique structure, HPB nanospheres have a great drug load rate and NIR photothermal conversion efficiency. Lu et al substituted Fe 2 O 3 nanospheres for the conventional FeCl 3 precursor, and then PBNPs were built into HPB nanospheres through an interface reaction [ 29 ]. Changing the growth period and template size under appropriate water and ethanol ratios allowed us to regulate the shell thickness and size of HPB nanospheres.…”

Combined Prussian Blue Nanozyme Carriers Improve Photodynamic Therapy and Effective Interruption of Tumor Metastasis

“… 35 , 36 Studies have shown that hollow Prussian blue nanoparticles can effectively load chemotherapy drugs and be used for tumor-targeted thermochemotherapy. 37–42 In addition, Prussian blue nanoparticles have the potential to be highly effective drug carriers due to their stable structure, good stability and long blood circulation time. 43 , 44 …”