&lt;p&gt;HPV-16 E2/E6 and POU5F1B as Biomarkers to Determine Cervical High-Grade Squamous Lesions and More&lt;/p&gt;

Chen, Lihua; Dong, Bin; Gao, Hangjing; Xue, Huifeng; Pan, Diling; Sun, Pengming

doi:10.2147/jir.s278911

Cited by 14 publications

(13 citation statements)

References 37 publications

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“…Third, blocking the trans -acting mediators released by POU5F1B-expressing cells could also be of benefit, as these molecules are predicted to increase the oncogenic properties of neighboring cells and of the tumor microenvironment. Finally, the presence of POU5F1B -encoding TcGTs in several other malignancies, including breast, prostate, stomach, and uterus, the previous identification of the POU5F1B locus as an integration hotspot for human papillomavirus and the coincidence of its transcripts with more advanced histological grades in cervical cancer 59 , 60 , as well as the documentation of its amplification in some gastric tumors 51 and growth-promoting effect on hepatocellular carcinoma cell lines 50 all indicate that our findings have a relevance that likely extends well beyond colorectal cancer alone.…”

Section: Discussionmentioning

confidence: 98%

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

et al. 2022

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The treatment of colorectal cancer (CRC) is an unmet medical need in absence of early diagnosis. Here, upon characterizing cancer-specific transposable element-driven transpochimeric gene transcripts (TcGTs) produced by this tumor in the SYSCOL cohort, we find that expression of the hominid-restricted retrogene POU5F1B through aberrant activation of a primate-specific endogenous retroviral promoter is a strong negative prognostic biomarker. Correlating this observation, we demonstrate that POU5F1B fosters the proliferation and metastatic potential of CRC cells. We further determine that POU5F1B, in spite of its phylogenetic relationship with the POU5F1/OCT4 transcription factor, is a membrane-enriched protein that associates with protein kinases and known targets or interactors as well as with cytoskeleton-related molecules, and induces intracellular signaling events and the release of trans-acting factors involved in cell growth and cell adhesion. As POU5F1B is an apparently non-essential gene only lowly expressed in normal tissues, and as POU5F1B-containing TcGTs are detected in other tumors besides CRC, our data provide interesting leads for the development of cancer therapies.

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Section: Discussionmentioning

confidence: 98%

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

et al. 2022

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“…The receiver operating characteristic (ROC) curve analysis was further performed to assess the diagnostic performance of the identified potential biomarkers. [30][31][32][33] The area under the curve (AUC) was the evaluation index. In general, an AUC of 0.5 to 07, 0.7 to 0.8, 0.8 to 0.9 and 0.9 to 1.0 were considered no discrimination, acceptable, excellent, and outstanding, respectively.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota-Derived Inflammation-Related Serum Metabolites as Potential Biomarkers for Major Depressive Disorder

Bai

Xie

Bai

et al. 2021

JIR

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Background Although many works have been conducted to explore the biomarkers for diagnosing major depressive disorder (MDD), the widely accepted biomarkers are still not identified. Thus, the combined application of serum metabolomics and fecal microbial communities was used to identify gut microbiota-derived inflammation-related serum metabolites as potential biomarkers for MDD. Methods MDD patients and healthy controls (HCs) were included in this study. Both serum samples and fecal samples were collected. The liquid chromatography mass spectrometry (LC-MS) was used to detect the metabolites in serum samples, and the 16S rRNA gene sequencing was used to analyze the gut microbiota compositions in fecal samples. Results Totally, 60 MDD patients and 60 HCs were recruited. The 24 differential serum metabolites were identified, and 10 of these were inflammation-related metabolites. Three significantly affected inflammation-related pathways were identified using differential metabolites. The 17 differential genera were identified, and 14 of these genera belonged to phyla Firmicutes. Four significantly affected inflammation-related pathways were identified using differential genera. Five inflammation-related metabolites (LysoPC(16:0), deoxycholic acid, docosahexaenoic acid, taurocholic acid and LysoPC(20:0)) were identified as potential biomarkers. These potential biomarkers had significant correlations with genera belonged to phyla Firmicutes. The panel consisting of these biomarkers could effectively distinguish MDD patients from HCs with an area under the curve (AUC) of 0.95 in training set and 0.92 in testing set. Conclusion These findings suggested that the disturbance of phyla Firmicutes might be involved in the onset of depression by regulating host’s inflammatory response, and these potential biomarkers could be useful for future investigating the objective methods for diagnosing MDD.

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“…Another study revealed that the expression of C-myc and POU5F1B is higher in CIN grade 2 or worse (CIN2+) than in normal patients or CIN1. 21,22 hTERT expression also increases in CIN2+. 23 Although these findings suggest that BV may play an important role in the progression of HR-HPV infection to cervical cancer, the relationship between cervicovaginal microbiota and persistent HR-…”

Section: Introductionmentioning

confidence: 93%

“…Altoos et al 20 demonstrated that the expression level of NF‐κBp65 is associated with poor clinical outcomes in patients with locally advanced cervical cancer. Another study revealed that the expression of C‐myc and POU5F1B is higher in CIN grade 2 or worse (CIN2+) than in normal patients or CIN1 21,22 . hTERT expression also increases in CIN2+ 23 …”

Section: Introductionmentioning

confidence: 99%

Prevotella as the hub of the cervicovaginal microbiota affects the occurrence of persistent human papillomavirus infection and cervical lesions in women of childbearing age via host NF‐κB/C‐myc

Dong

Huang

Cai

et al. 2022

Journal of Medical Virology

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There is evidence that coinfection of cervicovaginal high-risk human papillomavirus (HR-HPV) and bacteria is common in women of childbearing age. However, the relationship between bacterial vaginosis (BV) and persistent HR-HPV infection in women of childbearing age and the underlying mechanisms remain unclear. In this study, we determined whether BV affects persistent HR-HPV infection in women aged 20-45 years and explored the possible mechanisms of their interactions. From January 1 to April 30, 2020, we recruited women aged 20-45 years with and without BV at a ratio of 1:2 from Fujian Maternity and Child Health Hospital. All women were followed up at 0, 12, and 24 months. A BV assay, HR-HPV genotyping and cervical cytology were performed at each follow-up. At 0 months, additional vaginal secretions and cervical exfoliated cells were collected for 16S ribosomal RNA sequencing, bacterial metabolite determination, and POU5F1B, C-myc, TLR4, NF-κB, and hTERT quantification. A total of 920 women were included. The

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<p>HPV-16 E2/E6 and POU5F1B as Biomarkers to Determine Cervical High-Grade Squamous Lesions and More</p>

Cited by 14 publications

References 37 publications

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

Gut Microbiota-Derived Inflammation-Related Serum Metabolites as Potential Biomarkers for Major Depressive Disorder

Prevotella as the hub of the cervicovaginal microbiota affects the occurrence of persistent human papillomavirus infection and cervical lesions in women of childbearing age via host NF‐κB/C‐myc

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