<p>ICAM1 Regulates the Development of Gastric Cancer and May Be a Potential Biomarker for the Early Diagnosis and Prognosis of Gastric Cancer</p>

Abstract

Background: Gastric cancer (GC) is among the most common forms of cancer affecting the digestive system. This study sought to identify hub genes regulating early GC (EGC) in order to explore their potential for early diagnosis and prognosis of patients. Methods: We utilized a publically available dataset from the Gene Expression Omnibus database (GSE55696). Differences between EGC and LGIN with respect to gene expression were compared using the limma software. Identified differentially expressed genes (DEGs) were subjected to gene ontology (GO) and pathway enrichment analyses with the DAVID application, and the STRING website and Cytoscape software were used to construct a protein-protein interaction (PPI) network incorporating these DEGs. This network was in turn used to identify hub genes among selected DEGs, which were analyzed with the Kaplan-Meier Plotter database. In addition, Western blotting, qRT-PCR, immunohistochemistry, and UALCAN were all employed to validate the relationship between the expression of these genes and GC patient prognosis. Results: A total of 482 DEGs were identified, with GO analyses indicating an increase in the expression of genes linked with the development of cancer. Pathway analyses also indicated that these genes play a role in certain cancer-related pathways. The PPI network highlighted four potential hub genes, of which only ICAM1 was linked to a poor GC patient prognosis. This link between ICAM1 and GC patient outcomes was confirmed via UALCAN, Western blotting, immunohistochemistry, and qRT-PCR. Conclusion: ICAM1 may therefore modulate tumor progression in GC, thus potentially representing a valuable prognostic and diagnostic biomarker of EGC.

Bioinformatics analysis identifies biomarkers associated with poor prognosis in diffuse‑type gastric cancer

“…Reanalyzing and reintegrating such datasets often provides some meaningful insights for research. A number of microarray datasets of GC have been developed in recent years (15)(16)(17) and a large number of significant differentially expressed genes (DEGs) have already been identified.…”

Fusobacterium nucleatum colonization is associated with decreased survival of helicobacter pylori-positive gastric cancer patients

“…Not only genes involved in the regulation of immunological functions but also those related to cell mobility and adhesion were dysregulated by F. nucleatum colonization. For instance, epithelial-stromal interaction 1[ 25 , 26 ] and intercellular adhesion molecule 1[ 27 ], both of which are involved in cell-matrix interaction, were upregulated at 24 h and returned to basal levels after 72 h of incubation (Figure 2B ). Other cytoskeleton and cell adhesion genes were activated in a distinct pattern, and actin alpha 2; actin, alpha cardiac muscle precursor 1; actin gamma 2, smooth muscle; calponin 1, endothelin 1, and cell migration inducing hyaluronan binding protein were continuously stimulated by F. nucleatum (Figure 2C ).…”

Fusobacterium nucleatum Promotes Gastric Cancer Aggressiveness through Upregulation of Cell Mobility and Interferon Genes

“…In addition to the genes of immunological functions, our analysis also discovered that genes involved in cell mobility and adhesion were deregulated by Fusobacterium nucleatum infection. Among these genes are EPSTI1 [12,24] and ICAM1 [9], which are both involved in cell-matrix interaction; they were upregulated at 24 hours by high MOI treatment (Fig. 5).…”