<p>Identification of BRMS1L as Metastasis Suppressing Gene in Esophageal Squamous Cell Carcinoma</p>

Abstract

Introduction: Breast cancer metastasis suppressor 1 like (BRMS1-like)was first reported to be a component of the Sin3-HDAC complex, but the role in the progression of cancers was largely unknown. Our previous study reported that BRMS1L promoted the metastasis of breast cancer through facilitating the recruitment of HDAC complex to the promoter FZD10, and hence suppressing the transcription of FZD10. Methods: In this study, we detected the expression level of BRMS1L in esophageal squamous cell carcinoma (ESCC). The effect of BRMS1L in TE-1D (knockdown) and ECA-109 (overexpression) cell lines was explored by transwell assays, wound healing assays, and cell adhesion assays. Quantitative real-time PCR, Western blot analysis, and luciferase assays were used to detect the interaction of the CBP/P300-BRMS1L-ITGA7 axis. Results: In the present study, we found that knockdown of BRMS1L promoted the migration, invasion, and epithelial-mesenchymal transition (EMT). Conversely, overexpression of BRMS1L inhibited the migration and invasion of ESCC. Mechanistically, BRMS1L exerted their metastasis-suppressing role via transcriptionally repress ITGA7 expression. Moreover, we revealed that CBP/p 300 regulated the expression of BRMS1L and might be responsible for the down-regulation of BRMS1L in ESCC. Conclusion: Collectively, we identified the role of CBP/p300-BRMS1L-ITGA7 axis in the metastasis of ESCC.

Cancer chemoprevention through Frizzled receptors and EMT

“…BRMS1L knockdown increased levels of expression of FZD10 and induced EMT [ 105 ]. This mechanism also functions in esophageal squamous cell carcinoma [ 108 ]. In colorectal cancer (CRC), cells with high FZD10 expression were more efficient than cells with low FZD10 at stimulating EMT [ 134 ].…”

BRMS1L confers anticancer activity in non-small cell lung cancer by transcriptionally inducing a redox imbalance in the GPX2-ROS pathway

“…BRMS1L and p53 colocalize in cancer cells and synergistically inhibit the migration and invasion of cancer cells. BRMS1L also suppresses oesophageal squamous cell carcinoma metastasis by suppressing ITGA7 expression and is regulated by the transcription of CBP/P300 [12] . In addition, our previous papers have confirmed that BRMS1L inhibits EMT of tumour cells through the classical WNT-β-catenin signalling pathway, thereby preventing ovarian cancer metastasis [21] .…”

“…used immunohistochemical staining to reveal that BRMS1L shows high expression in normal breast tissues and low expression in breast cancer, and its expression is gradually decreased and lost in accordance with distant metastasis of breast cancer [11] . In addition, low expression of BRMS1L has also been found in several other malignant tumours [12] , [13] , [14] , [15] , [16] . Functional experiments in breast cancer revealed that BRMS1L affects the invasion and migration capabilities of breast cancer cells by inhibiting EMT.…”

Machine learning-identified stemness features and constructed stemness-related subtype with prognosis, chemotherapy, and immunotherapy responses for non-small cell lung cancer patients

“…Relative gene expression was analyzed by the 2-ΔΔCt method, using Gapdh as an internal control. qPCR and in vitro cancer cell function assays were also performed as described previously [ 33 , 34 ]. qPCR primer sequences are shown in Additional file 1 : Table S3.…”