<p>Indocyanine Green and Curcumin Co-Loaded Nano-Fireball-Like Albumin Nanoparticles Based on Near-Infrared-Induced Hyperthermia for Tumor Ablation</p>

Abstract

Background: Indocyanine green (ICG) has received considerable interest as a biocompatible organic photothermal agent, and curcumin (Cur) is considered an attractive natural chemopreventive and chemotherapeutic compound. However, the in vivo applicability of ICG and Cur is significantly restricted by their poor ability to target tumors and their extremely low solubility. Materials and Methods: To address these problems, ICG/Cur-loaded albumin nanoparticles (ICG-BSA-Cur-NPs) based on the nab TM (nanoparticle albumin-bound) technology were applied to neuroblastomas in vivo. Results: The fabricated ICG-BSA-Cur-NPs were found to be spherical, ~150 nm in size and highly dispersible and stable in aqueous solution. Approximately 80% of the incorporated ICG and Cur were gradually released from the NPs over 48 h. All formulations of ICG-BSA-Cur-NPs (5~20 µg/mL) showed efficient hyperthermia profiles (up to 50-60°C within 5 min) in response to 808-nm NIR laser irradiation in vitro and in vivo. Notably, ICG-BSA-Cur-NPs illuminated with 808-nm laser irradiation (1.5 W/cm 2) showed excellent cytotoxicity toward N2a cells in vitro and undisputable antitumor efficacy in N2a-xenografted mice in vivo, compared to other tested sample groups (tumor volumes for PBS, BSA-Cur-NPs, free ICG, and ICG-BSA-Cur-NPs groups were 1408.6 ± 551.9, 1190.6 ± 343.6, 888.6 ± 566.2, and 103.0 ± 111.3 mm 3 , respectively). Conclusion: We demonstrate that these hyperthermal chemotherapeutic ICG-BSA-Cur-NPs have potential as a future brain tumor treatment.

Photoreactive-proton-generating hyaluronidase/albumin nanoparticles-loaded PEG-hydrogel enhances antitumor efficacy and disruption of the hyaluronic acid extracellular matrix in AsPC-1 tumors

“…The o-NBA/HAase-HSA-NPs were fabricated as previously described using the nanoparticle albumin-bound (nab™) technique with slight modifications [ 22 , 23 , 25 ]. The nab™ technology is a well-known preparation method to encapsulate hydrophobic drugs into albumin through an emulsion-evaporation cross-link method using high-pressure homogenization without the use of Cremophor®.…”

“…Previously, we developed a series of nanoparticle-albumin-bound (nab T M )-based formulations to suppress many solid tumors [ [20] , [21] , [22] , [23] ]. Furthermore, we introduced modified human serum albumin nanoparticles (HSA-NPs) embedded with macromolecules, such as tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) and hyaluronidase [ 24 , 25 ].…”

Combined Antitumor Therapy Using In Situ Injectable Hydrogels Formulated with Albumin Nanoparticles Containing Indocyanine Green, Chlorin e6, and Perfluorocarbon in Hypoxic Tumors

“…The ICG/PTX/BSA-Ce6-NPs were prepared as previously described using the nanoparticle albumin-bound (nab TM ) technology with some adjustments [2,[30][31][32][33][34]. In brief, 45 mg BSA, 5 mg BSA-Ce6, and 0.75 mg ICG were dissolved in 5 mL DW.…”

“…2.12. Cytotoxicity of ICG/PTX/BSA-Ce6-NPs in 4T1 Cell Spheroids Using LIVE/DEAD TM Cell Assay Three-dimensional culture of 4T1 cells to form spheroids was performed by a slight modification of previously described methods [2,26,30]. LIVE/DEAD™ viability/cytotoxicity kits were used to visualize the death and viability of the 4T1 cells.…”

Emerging indocyanine green-integrated nanocarriers for multimodal cancer therapy: a review

“…Curcumin is a natural compound, is derived from turmeric and it has a potent chemopreventive and chemotherapeutic agent. Finally, these NPs were analyzed with neuroblastoma N2a cells for in vitro xenografted nude mice used for in vivo85 . Sheng and their group were developed HSA and ICG NPs (HSA-ICG NPs) by intermolecular disulfide conjugations.…”