<p>KIAA1522 Promotes the Progression of Hepatocellular Carcinoma via the Activation of the Wnt/β-Catenin Signaling Pathway</p>

Abstract

KIAA1522 was previously identified to play a crucial role in cancer development and progression. However, its functions and underlying mechanisms in hepatocellular carcinoma (HCC) remain elusive. Materials and Methods: To elucidate the role of KIAA1522 in HCC, its expression was assessed using The Cancer Genome Atlas and GEPIA databases. Next, these results were validated by quantitative reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry of HCC tissues and cell lines. Flow cytometry, CCK-8, EDU, colony formation, Transwell invasion, and wound healing assays were performed to explore the function of KIAA1522 in HCC in vivo and in vitro. Finally, gene set enrichment analysis was used to identify the pathways involved. Results: Our results demonstrated that KIAA1522 was highly expressed in HCC tissues and cell lines. Furthermore, KIAA1522 overexpression was associated with unfavorable clinicopathological characteristics. Survival analyses revealed that KIAA1522 overexpression predicted lower recurrence-free and overall survival rates in patients with HCC. Functional studies suggested that KIAA1522 facilitated HCC proliferation, migration, and invasion both in vitro and in vivo. Moreover, KIAA1522 up-regulated the Wnt/β-catenin signaling pathway, as confirmed by TOP-flash/FOP-flash luciferase reporter assays and Western blotting. Conclusion: In conclusion, we highlighted the oncogenic role of KIAA1522 in HCC and determined its potential as a therapeutic target for HCC.

Poor Prognostic Biomarker KIAA1522 Is Associated with Immune Infiltrates in Hepatocellular Carcinoma

“…In order to account for the effects of age and gender on unfavorable outcomes, AFP in serum, univariate, and multivariate analyses were carried out, which suggests that KIAA1522 is a possible prognostic and diagnostic marker. Recent reports [ 14 , 15 ] agree with the findings.…”

“…The mRNA expression of KIAA1522 was elevated in a number of malignancies, according to data from the Gene Expression Atlas and The European Bioinformatics Institute databases, which showed that abnormal KIAA1522 expression may have a role in the pathogenesis and growth of cancer [ 13 ]. Recent research demonstrated that elevated KIAA1522 expression accelerated the evolution of HCC by activating the Wnt/ β -catenin pathway and predicted a poor prognosis in HCC patients [ 14 , 15 ]. The function of KIAA1522 in human cancer immunology, however, has hardly ever been thoroughly examined.…”

Development of novel DNAJB6-KIAA1522-p-mTOR three-protein prognostic prediction models for CRC

“…The above findings also suggested that KIAA1522 might be a potential therapeutic target in CRC. Functional studies revealed that high expression of KIAA1522 promoted the proliferation, survival, migration and invasion of CRC cells [48] . Whether KIAA1522 has other functional roles in CRC cells, such as regulating the cell cycle and EMT, remains to be further investigated.…”

New Endothelial Corneal Dystrophy in a Chinese Family

“…It is a newly cloned gene with unknown functions. 25 In addition, there have been a few functional studies documenting that overexpression of KIAA1522 is associated with human cancers, including esophageal squamous cell carcinoma, Ewing sarcoma, and nonsmall cell lung cancer. [26][27][28][29][30] These studies demonstrated that KIAA1522 plays a critical role in cell adhesion and apoptosis inhibition.…”