<p>KRT17 Functions as a Tumor Promoter and Regulates Proliferation, Migration and Invasion in Pancreatic Cancer via mTOR/S6k1 Pathway</p>

Abstract

Background: Pancreatic cancer (PC) is one of the most well-known malignancies with high mortality, but the underlying mechanism of PC remains unknown. Keratin17 (KRT17) expression has been reported in many malignancies, but its functions in PC are not clear. The aim of our study was to evaluate KRT17 expression and its potential role in PC. Methods: The online databases GEPIA and THPA were used to identify KRT17 expression in tissues. Quantitative real-time PCR (qRT-PCR) was used to determine KRT17 expression in cell lines. Ki67 and ROS levels were detected by immunofluorescence assay and a 2ʹ,7ʹdichlorodihydrofluorescein diacetate (DCFH-DA) probe. KRT17 downregulation was induced by the small interfering RNA (siRNA) technique. Proliferation function was evaluated by colony formation assay and RTCA. Migration and invasion were evaluated by transwell migration assay. A Western blot assay was used to detect protein levels. Results: KRT17 was overexpressed in PC tissues compared to that in normal tissues. The results showed that Ki67 and ROS levels were decreased in pancreatic cancer cells after transfection with siKRT17. After KRT17 downregulation in PC cell lines, cell viability functions, including proliferation, migration and invasion, and mTOR/S6K1 phosphorylation levels were attenuated. Conclusion: KRT17 knockdown significantly inhibited proliferation, migration and invasion in pancreatic cancer cells.

Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway

“…Similarly, the pro-proliferation function of KRT17 was also observed in pancreatic cancer cell line [21]. It was demonstrated that pancreatic cancer cells transfected with KRT17 siRNA showed lower Reactive-Oxygen-Species and mTOR/S6K1 phosphorylation levels, as well as reduced proliferation, migration and invasion [25]. In lung adenocarcinoma, overexpression of KRT17 is closely associated with advanced TNM stage and poor overall survival, and KRT17 depletion remarkably suppresses cancer cell proliferation and invasion in vitro [26].…”

Low Expression of Keratin17 is Related to Poor Prognosis in Bladder Cancer

“…KRT17 is also involved in the occurrence and development of a variety of tumors or is associated with poor prognosis of a variety of tumors, such as colorectal cancer, 19 gastric cancer, 20 cervical cancer, 21 renal cell carcinoma, 22 pancreatic cancer, 23 non-small cell cancer, 24 etc., but no related research has been conducted in bladder cancer.…”

“… 17 KRT17 was initially studied in basal cell carcinoma of the skin, which belongs to type I keratin family with a molecular weight of about 48kd. 18 Existing studies have shown that KRT17 is involved in the occurrence and development of a variety of tumors or guide the different prognosis of many tumors, such as colorectal cancer, 19 gastric cancer, 20 cervical cancer, 21 renal cell carcinoma, 22 pancreatic cancer, 23 non-small cell cancer, 24 etc. However, the role of KRT17 in the prognosis of bladder cancer and its possible pathogenesis are still unclear.…”

Exploring the Mechanism of Coagulation Dysfunction in a Sepsis Model though Gene Expression Profiles

“…Sample preparation for qRT-PCR was performed as described previously (16,27). RNA expression was detected by qRT-PCR which was performed with qPCR Master Mix(TOROGReen, Toroivd ) and 7500 Fast machine (Applied Biosystems).…”