&lt;p&gt;LINC00491 as a new molecular marker can promote the proliferation, migration and invasion of colon adenocarcinoma cells&lt;/p&gt;

Wan, Jiahui; Deng, Daiqian; Wang, Xiuli; Shu, Jiangbo; Cui, Rongjun

doi:10.2147/ott.s201233

Cited by 29 publications

(24 citation statements)

References 34 publications

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“…10 In addition, similar to protein-coding genes, lncRNA has important roles in diverse cellular processes by silencing miRNA, which was a canonical competing endogenous RNA (ceRNA) regulating pattern; moreover, frequent dysregulation of lncRNA already associated with many vital cellular processes and pathogenesis of tumors. 11,12 A study has shown that abnormal expression of lncRNA participated in initiation, progression, metastasis, and drug resistance of human malignancies. 13 For instance, colon cancer-associated transcript-1 (CCAT1) has been defined as an oncogenic lncRNA in glioma and acute myeloid leukemia.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma</p>

Fan

Kang

Zhao

et al. 2020

CMAR

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Background: Melanoma is aggressive and lethal melanocytic neoplasm, and its incidence has increased worldwide in recent decades. Accumulating evidence has showed that various long noncoding RNAs (lncRNAs) participated in occurrence of malignant tumors, including melanoma. The present study was designed to investigate function of lncRNA colon cancerassociated transcript-1 (CCAT1) in melanoma. Methods: The expression levels of CCAT1, miR-296-3p and Integrin alpha9 (ITGA9) in melanoma tissues or cells were measured using real-time quantitative polymerase chain reaction (RT-qPCR). The concentrations of glucose and lactate were measured for assessing glycolysis of melanoma cells. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazol-3-ium bromide (MTT), flow cytometry, and transwell assays were conducted to assess proliferation, apoptosis, and migration of melanoma cells. Western blot assay was performed to measure the protein expression of ITGA9, hexokinase 2 (HK2), and epithelial-mesenchymal transition (EMT)-related proteins in melanoma tissues or cells. The relationship among CCAT1, miR-296-3p, and ITGA9 was predicted and confirmed by bioinformatics analysis, dualluciferase reporter, and RNA immunoprecipitation (RIP) assay, respectively. A xenograft experiment was established to assess the effect of CCAT1 knockdown in vivo. Results: CCAT1 was effectively increased in melanoma tissues and cells compared with matched controls, and deficiency of CCAT1 impeded cell glycolysis, proliferation, migration while induced apoptosis, which were abrogated by knockdown of miR-296-3p in melanoma cells. In addition, our findings revealed that ITGA9 overexpression abolished miR-296-3p overexpression-induced effects on melanoma cells. Importantly, CCAT1 regulated ITGA9 expression by sponging miR-296-3p. The results of xenograft experiment suggested that CCAT1 silencing inhibited melanoma cell growth in vivo. Conclusion: LncRNA CCAT1 promoted ITGA9 expression by sponging miR-296-3p in melanoma.

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Section: Introductionmentioning

confidence: 99%

<p>Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma</p>

Fan

Kang

Zhao

et al. 2020

CMAR

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“…Similar results were obtained by Nie et al [33], whose expression analysis of miR-365 by qPCR was also decreased in the CRC cell lines, HT29, LoVo, SW480 and SW620, compared with normal colon mucosa tissues. Wan et al [53] also corroborated by PCR that the expression levels of miR-145 were significantly suppressed in the CRC cell lines, HT-29 and HCT116 cells. In order to make the assays with sponges we chose 3 CRC cell lines: the two lines with higher expression of both miRNAs (RKO and T84) and the line with lower expression (HCT116).…”

Section: Discussionmentioning

confidence: 78%

Novel miRNA Sponges to Specifically Modulate Gene Expression in Colon Cancer Cells

Aguilera¹,

Perazzoli²,

Cabeza³

et al. 2019

Preprint

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miRNA sponges allow the selective blockade of a complete family of associated miRNAs which induce posttranscriptional gene silencing in its target through binding to 3´UTR mRNA. MiRNA-365 and miRNA-145 are down-regulated in colorectal cancer (CRC), but not in health tissues. Based on this, we constructed two vectors by inserting miRNA sponge (one for miRNA-365 and other for miRNA-145), and used EGFP (enhanced green fluorescent protein) as a 3′ UTR reporter gene to analyse the ability of each sponge to catch its respective miRNA. qPCR results corroborated that the expression levels of both miRNAs were lower in CRC cell lines than in normal colon cell line. Flow cytometry analysis revealed a decrease of the EGFP expression levels in the cell lines transfected with both sponges, being higher on the normal cell line while CRC cell lines presented a minimal decline. Also, this decrease was inversely proportional to the levels of expression of both miRNAs obtained by qPCR. These results were corroborated by fluorescence microscopy, showing a similar decrease fluorescence. We propose a new vector system to carry in a specific way the expression of genes to CRC cells without affecting healthy cells, preventing damage to healthy tissues.

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“…ENST00000510145 is one of nine transcripts of gene ENSG00000250682, also known as LINC00491 or BC008363. This gene was found to be upregulated in a TCGA colon adenocarcinoma dataset and was associated with lower patient survival, implying ENSG00000250682 importance for colorectal cancer progression 53 . Conversely, in pancreatic ductal adenocarcinoma patients LINC00491 expression was significantly lower compared to the control group and was associated with better survival rates 54 .…”

Section: Resultsmentioning

confidence: 99%

Identification of a long non-coding RNA regulator of liver carcinoma cell survival

et al. 2021

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Genomic studies have significantly improved our understanding of hepatocellular carcinoma (HCC) biology and have led to the discovery of multiple protein-coding genes driving hepatocarcinogenesis. In addition, these studies have identified thousands of new non-coding transcripts deregulated in HCC. We hypothesize that some of these transcripts may be involved in disease progression. Long non-coding RNAs are a large class of non-coding transcripts which participate in the regulation of virtually all cellular functions. However, a majority of lncRNAs remain dramatically understudied. Here, we applied a pooled shRNA-based screen to identify lncRNAs essential for HCC cell survival. We validated our screening results using RNAi, CRISPRi, and antisense oligonucleotides. We found a lncRNA, termed ASTILCS, that is critical for HCC cell growth and is overexpressed in tumors from HCC patients. We demonstrated that HCC cell death upon ASTILCS knockdown is associated with apoptosis induction and downregulation of a neighboring gene, protein tyrosine kinase 2 (PTK2), a crucial protein for HCC cell survival. Taken together, our study describes a new, non-coding RNA regulator of HCC.

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<p>LINC00491 as a new molecular marker can promote the proliferation, migration and invasion of colon adenocarcinoma cells</p>

Cited by 29 publications

References 34 publications

<p>Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma</p>

<p>Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma</p>

Novel miRNA Sponges to Specifically Modulate Gene Expression in Colon Cancer Cells

Identification of a long non-coding RNA regulator of liver carcinoma cell survival

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